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anti-Human NFAT5 antibody for Immunoprecipitation

Recommended NFAT5 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) Antibodies
  • AI225870
  • B130038B15Rik
  • CAG-8
  • CAG80
  • DKFZp469P1514
  • mKIAA0827
  • nf-at5
  • NF-AT5
  • Nfat
  • NFAT5
  • NFATL1
  • nfatl1
  • NFATZ
  • nfatz
  • OREBP
  • orebp
  • tonebp
  • TONEBP
  • TonEBP
  • wu:fi46f11
  • zgc:63502
Epitope
C-Term
16
7
7
6
3
3
2
2
2
2
1
1
1
Reactivity
Human
68
45
30
Host
Rabbit
58
12
Clonality
Polyclonal
Conjugate
This NFAT5 antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
36
29
22
12
11
5
3
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Available images

Catalog No. ABIN267152
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Similar anti-NFAT5 Antibodies

Application / Reactivity Human
ELISA 28 Antibodies
Gel Shift (GS) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 13 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 35 Antibodies

General

Antigen Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) Antibodies
Epitope C-Term
(16), (7), (7), (6), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human
(68), (45), (30)
Host Rabbit
(58), (12)
Clonality
Polyclonal
Conjugate This NFAT5 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(36), (29), (22), (12), (11), (5), (3)
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Product Details anti-NFAT5 Antibody

Target Details NFAT5 Application Details Handling Images
Cross-Reactivity (Details) Predicted cross-reactivity based on sequence identity: Mouse (94%).
Purification IgG purified
Immunogen Synthetic peptide: CD(1439)LLVSLQNQGNNLTGSF(1455).Immunizing peptide corresponds to the C-terminal 17 amino acid residues of human NFAT 5.
Isotype IgG

Target Details NFAT5

Product Details anti-NFAT5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NFAT5 (NFAT5 Antibody Abstract)
Background The nuclear factor of activated T-cells (NFAT) transcription complex is required for theexpression of a group of proteins that collectively regulate the immune response. FourNFAT proteins, encoded on separate genes and expressed as several splicevariants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2(NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show alow level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors.Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacksmany of the Fos/Jun contact sites observed in its predecessors and its subcellularlocalization is not calcineurin-dependent. Alternate Names: anti-Glutamine rich protein H65 antibody, anti-KIAA0827 antibody, anti-NF AT5antibody, anti-NFAT 5 antibody, anti-NFAT L1 antibody, anti-NFAT like protein 1antibody, anti-NFATL 1 antibody,anti-NFATL1 antibody ,anti-NFATZ antibody ,anti-Nuclear factor of activated T cells 5 antibody, anti-Nuclear factor of activated T cells5 tonicity responsive antibody, anti-Nuclear factor of activated T cells antibody,anti-OREBP antibody, anti-Osmotic response element binding protein antibody, anti-Tcell transcription factor NFAT 5 antibody, anti-T cell transcription factor NFAT5 antibody,anti-TonE binding protein antibody, anti-TONEBP antibody, anti-Tonicity responsiveenhancer binding protein antibody.
Gene Symbol: NFAT5
Gene ID 10725
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Adaptive Immunity
Pathways RTK Signaling, WNT Signaling

Application Details

Product Details anti-NFAT5 Antibody Target Details NFAT5 Handling Images back to top
Application Notes By Western blot, this antibody detects an ~170 kDa protein representing NFAT 5 in HEK293 cells transfected with the human NFAT 5 gene. Immunocytochemical staining of NFAT 5 in HEK293 cells transfected with the human NFAT5 gene results in primarily cytoplasmic staining.
Recommended dilutions: Gel Super Shift Assays 1:1-1:100, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:20, Immunoprecipitation 3 µg, Western Blot 1:1000
Restrictions For Research Use only

Handling

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details Images back to top
Buffer PBS containing 1 mg/ml BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (C-Term) (ABIN267152) Figure 1 shows a Western blot of human NFAT5 from transfected BHK cell lysate with AB...