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anti-Human NFAT5 antibody for Western Blotting

Recommended NFAT5 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) Antibodies
  • zgc:63502
  • wu:fi46f11
  • TonEBP
  • NFAT5
  • nfatz
  • orebp
  • nf-at5
  • nfatl1
  • tonebp
  • DKFZp469P1514
  • AI225870
  • B130038B15Rik
  • CAG-8
  • CAG80
  • NFATL1
  • OREBP
  • mKIAA0827
  • NF-AT5
  • NFATZ
  • TONEBP
  • Nfat
  • nuclear factor of activated T-cells 5, tonicity-responsive
  • nuclear factor of activated T cells 5
  • NFAT5
  • nfat5
  • Nfat5
Epitope
Middle Region
18
7
6
6
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
72
52
36
3
2
2
2
1
1
1
1
Host
Rabbit
62
12
Clonality
Polyclonal
Conjugate
This NFAT5 antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Western Blotting (WB)
36
28
26
12
12
8
4
1
1
1
Options
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Catalog No. ABIN2780794
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0726016 ABIN2780105 WB Rabbit Middle Region Log in to see Polyclonal 1
3.0726016 ABIN267152 ChIP GS ICC IF IHC IHC (p) IP WB Rabbit IgG Log in to see Polyclonal 2
3.0726016 ABIN1532160 ELISA WB Rabbit IgG pSer155 Log in to see Polyclonal
3.0726016 ABIN3222948 IHC WB Rabbit N-Term Log in to see Polyclonal
3.0726016 ABIN524154 IF WB Mouse AA 1-1484, full length Log in to see Polyclonal
3.0726016 ABIN948983 IF WB Mouse AA 1-1484, full length Log in to see Polyclonal
3.0726016 ABIN4894433 WB Rabbit Log in to see Polyclonal
3.0726016 ABIN927663 WB Rabbit Middle Region Log in to see Polyclonal
1 ABIN468412 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN467533 IHC (p) WB Rabbit AA 41-298 Log in to see Polyclonal
1 ABIN3182367 ELISA WB Rabbit IgG pSer155 Log in to see Polyclonal
1 ABIN226195 ICC WB Rabbit IgG AA 1534-1549 Log in to see Polyclonal
1 ABIN2755584 IHC WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1917019 ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 AA 1422-1532 Log in to see
1 ABIN1917020 ELISA WB APC Mouse IgG1 AA 1422-1532 Log in to see
1 ABIN1917021 ELISA WB FITC Mouse IgG1 AA 1422-1532 Log in to see
1 ABIN1917022 ELISA WB PE Mouse IgG1 AA 1422-1532 Log in to see
1 ABIN1917023 ELISA WB Biotin Mouse IgG1 AA 1422-1532 Log in to see
1 ABIN1420798 IHC (p) WB HRP Rabbit IgG pSer1197 Log in to see Polyclonal
1 ABIN2565718 ELISA WB Mouse IgG2a kappa AA 1422-1531, partial Log in to see 1H16

Top referenced anti-NFAT5 antibody for Western Blotting

Similar anti-NFAT5 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
ELISA 27 Antibodies
Gel Shift (GS) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 35 Antibodies

General

Antigen Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) Antibodies
Epitope Middle Region
(18), (7), (6), (6), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(72), (52), (36), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(62), (12)
Clonality
Polyclonal
Conjugate This NFAT5 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Western Blotting (WB)
(36), (28), (26), (12), (12), (8), (4), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-NFAT5 Antibody

Target Details NFAT5 Application Details Handling References for anti-NFAT5 Antibody (ABIN2780794) Images
Blocking Peptide Blocking peptide for this product available: ABIN980637
Sequence PGQPQNEGQP PVTTLLSQQM PENSPLASSI NTNQNIEKID LLVSLQNQGN
Predicted Reactivity Guinea pig : 100 %, Human : 100 %, Bovine : 90 %, Rabbit : 90 %, Dog : 80 %, Horse : 80 %, Pig : 80 %
Characteristics This is a rabbit polyclonal antibody against NFAT5. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human NFAT5

Target Details NFAT5

Product Details anti-NFAT5 Antibody Application Details Handling References for anti-NFAT5 Antibody (ABIN2780794) Images back to top
Antigen
Alternative Name NFAT5 (NFAT5 Antibody Abstract)
Background NFAT5 is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements.The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 166 kDa
Gene ID 10725
NCBI Accession NM_006599, NP_006590
UniProt O94916
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Adaptive Immunity
Pathways RTK Signaling, WNT Signaling

Application Details

Product Details anti-NFAT5 Antibody Target Details NFAT5 Handling References for anti-NFAT5 Antibody (ABIN2780794) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1531 AA

Restrictions For Research Use only

Handling

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details References for anti-NFAT5 Antibody (ABIN2780794) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-NFAT5 Antibody (ABIN2780794)

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details Handling Images back to top
Background publications

Zhou, Ferraris, Dmitrieva, Liu, Burg: "MKP-1 inhibits high NaCl-induced activation of p38 but does not inhibit the activation of TonEBP/OREBP: opposite roles of p38alpha and p38delta." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 14, pp. 5620-5, 2008

Images

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details Handling References for anti-NFAT5 Antibody (ABIN2780794) back to top
Supplier Images
Western Blotting (WB) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (Middle Region) (ABIN2780794) WB Suggested Anti-NFAT5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: