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anti-Rat (Rattus) RHEB antibody for ELISA

Recommended RHEB Antibody (supplied by: Log in to see )

Antigen
Ras Homolog Enriched in Brain (RHEB) Antibodies
  • im:7161941
  • RHEB
  • rheb2
  • RHEB2
  • zgc:153231
Epitope
N-Term
13
9
8
8
8
8
6
3
3
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
133
73
53
1
1
1
1
Host
Rabbit
88
45
Clonality
Polyclonal
Conjugate
This RHEB antibody is un-conjugated
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
111
31
26
16
11
7
5
3
1
Supplier
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Catalog No. ABIN1003086
$ 455.07
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012117105 ABIN1003087 IHC ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 4
0.0012117105 ABIN1031539 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012117105 ABIN2435078 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN2433457 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN1091350 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN1091351 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN1031063 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal
0.0012117105 ABIN2430735 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN2431049 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN2425557 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN2424077 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN2499808 IF IHC ELISA WB Rabbit IgG Middle Region Log in to see Polyclonal
0.0012117105 ABIN2499807 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012117105 ABIN1887872 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012117105 ABIN2964045 IHC (p) ELISA WB Mouse IgG1 kappa Log in to see 2C11
0.0012117105 ABIN1887803 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal
0.0012117105 ABIN1585138 ELISA WB Rabbit Log in to see Polyclonal
0.0012117105 ABIN1585139 IHC ELISA WB Rabbit Log in to see Polyclonal
0.0012117105 ABIN2959903 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012117105 ABIN2959902 IF IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal

Top referenced anti-RHEB antibody for ELISA

Similar anti-RHEB Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 112 Antibodies 56 Antibodies 36 Antibodies
Immunoprecipitation (IP) 7 Antibodies 1 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies 9 Antibodies 9 Antibodies
Immunohistochemistry (IHC) 26 Antibodies 16 Antibodies 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies 11 Antibodies
Immunofluorescence (IF) 5 Antibodies 4 Antibodies

General

Antigen Ras Homolog Enriched in Brain (RHEB) Antibodies
Epitope N-Term
(13), (9), (8), (8), (8), (8), (6), (3), (3), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(133), (73), (53), (1), (1), (1), (1)
Host Rabbit
(88), (45)
Clonality
Polyclonal
Conjugate This RHEB antibody is un-conjugated
(3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(111), (31), (26), (16), (11), (7), (5), (3), (1)
Pubmed 4 references available
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Product Details anti-RHEB Antibody

Target Details RHEB Application Details Handling References for anti-RHEB Antibody (ABIN1003086) Images
Blocking Peptide Blocking peptide for this product available: ABIN1004182
Purification Affinity chromatography purified via peptide column.
Immunogen Rabbit Rheb (NT) polyclol antibody was raised against a 14 amino acid peptide from the amino terminus of human Rheb (Genbank accession No. AAH16155).
Isotype IgG

Target Details RHEB

Product Details anti-RHEB Antibody Application Details Handling References for anti-RHEB Antibody (ABIN1003086) Images back to top
Antigen
Alternative Name Rheb (Ras Homolog Enriched in Brain) (RHEB Antibody Abstract)
Background Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.
Pathways RTK Signaling

Application Details

Product Details anti-RHEB Antibody Target Details RHEB Handling References for anti-RHEB Antibody (ABIN1003086) Images back to top
Application Notes Rheb antibody can be used for the detection of Rheb by Western blot at 2 - 4 µg/ml. (Optimal dilution should be determined by user.) Post-translationally modified Rheb is sometimes observed at higher molecular weight. Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only

Handling

Product Details anti-RHEB Antibody Target Details RHEB Application Details References for anti-RHEB Antibody (ABIN1003086) Images back to top
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months

References for anti-RHEB Antibody (ABIN1003086)

Product Details anti-RHEB Antibody Target Details RHEB Application Details Handling Images back to top
Product cited in:

Inoki, Li, Xu, Guan: "Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling." in: Genes & development, Vol. 17, Issue 15, pp. 1829-34, 2003

Stocker, Radimerski, Schindelholz, Wittwer, Belawat, Daram, Breuer, Thomas, Hafen: "Rheb is an essential regulator of S6K in controlling cell growth in Drosophila." in: Nature cell biology, Vol. 5, Issue 6, pp. 559-65, 2003

Yee, Worley: "Rheb interacts with Raf-1 kinase and may function to integrate growth factor- and protein kinase A-dependent signals." in: Molecular and cellular biology, Vol. 17, Issue 2, pp. 921-33, 1997

Background publications

Yamagata, Sanders, Kaufmann, Yee, Barnes, Nathans, Worley: "rheb, a growth factor- and synaptic activity-regulated gene, encodes a novel Ras-related protein." in: The Journal of biological chemistry, Vol. 269, Issue 23, pp. 16333-9, 1994