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|Antigen||Son of Sevenless Homolog 1 (Drosophila) (SOS1) Antibodies|
|Epitope||AA 1-109 Alternatives|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This SOS1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
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Product Details anti-SOS1 AntibodyTarget Details SOS1 Application Details Handling References for anti-SOS1 antibody (ABIN967741) Images
|Cross-Reactivity||Rat (Rattus), Dog (Canine), Human|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Mouse mSos1 aa. 1-109|
Target Details SOS1Product Details anti-SOS1 Antibody Application Details Handling References for anti-SOS1 antibody (ABIN967741) Images back to top
|Alternative Name||Sos1 (SOS1 Antibody Abstract)|
|Background||The Sos (son of sevenless) gene was originally identified in Drosophila, and two mammalian homologues (mSos1 and mSos2) were isolated from a mouse cDNA library. These two cDNAs predict proteins that are approximately 70% identical in their amino acid residues. Both mSos1 and mSos2 are expressed in a wide number of mouse embryonic and adult tissues as well as in several cell lines. The human homologues, hSos1 and hSos2 have also been isolated and show a very high degree of amino acid identity to the mouse genes (98% for Sos1). Sos1 has a predicted molecular weight of 150kDa, but the apparent molecular weight is closer to 170 kDa, presumably due to a high proline content. The mammalian Sos1 protein has a highly specific guanine nucleotide exchange activity toward p21ras . In EGF-stimulated cells, Sos1 interacts with the SH3 domains of GRB2, and GRB2 binds via its SH2 domain to tyrosine 1068 of the activated EGF receptor. Thus, GRB2 recruits Sos1 to the plasma membrane and enables it to activate the Ras signaling pathway by enhancing GTP loading on p21ras .|
|Molecular Weight||170 kDa|
|Pathways||RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-SOS1 Antibody Target Details SOS1 Handling References for anti-SOS1 antibody (ABIN967741) Images back to top
Related Products: ABIN968539, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-SOS1 Antibody Target Details SOS1 Application Details References for anti-SOS1 antibody (ABIN967741) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-SOS1 antibody (ABIN967741)Product Details anti-SOS1 Antibody Target Details SOS1 Application Details Handling Images back to top
|Product cited in:||
Furuta, Miura, Copeland, Shang, Oshima, Kamata: "Light Chain 3 associates with a Sos1 guanine nucleotide exchange factor: its significance in the Sos1-mediated Rac1 signaling leading to membrane ruffling." in: Oncogene, Vol. 21, Issue 46, pp. 7060-6, 2002
Kardinal, Konkol, Lin, Eulitz, Schmidt, Estrov, Talpaz, Arlinghaus, Feller: "Chronic myelogenous leukemia blast cell proliferation is inhibited by peptides that disrupt Grb2-SoS complexes." in: Blood, Vol. 98, Issue 6, pp. 1773-81, 2001
Salojin, Zhang, Meagher, Delovitch: "ZAP-70 is essential for the T cell antigen receptor-induced plasma membrane targeting of SOS and Vav in T cells." in: The Journal of biological chemistry, Vol. 275, Issue 8, pp. 5966-75, 2000
Buday, Downward: "Epidermal growth factor regulates p21ras through the formation of a complex of receptor, Grb2 adapter protein, and Sos nucleotide exchange factor." in: Cell, Vol. 73, Issue 3, pp. 611-20, 1993
Egan, Giddings, Brooks, Buday, Sizeland, Weinberg: "Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation." in: Nature, Vol. 363, Issue 6424, pp. 45-51, 1993
ImagesProduct Details anti-SOS1 Antibody Target Details SOS1 Application Details Handling References for anti-SOS1 antibody (ABIN967741) back to top