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Mouse (Murine) TEK ELISA Kit

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Antigen
TEK Tyrosine Kinase, Endothelial (TEK) ELISA Kits
  • AA517024
  • CD202B
  • Cd202b
  • DKFZp469E064
  • Hyk
  • TEK
  • Tie-2
  • TIE-2
  • tie-2
  • TIE2
  • tie2
  • Tie2
  • VMCM
  • VMCM1
Epitope
AA 23-744
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
29
17
8
3
3
2
2
2
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
125-8000 pg/mL
Minimum Detection Limit
125 pg/mL
Application
ELISA
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Catalog No. ABIN1672877
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32666028 ABIN415709 Sandwich ELISA Serum, Plasma, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
0.32666028 ABIN1030102 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.32666028 ABIN2708608 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 125-8000 pg/mL 125 pg/mL Log in to see
0.0012121415 ABIN367763 Sandwich ELISA Serum, Plasma, Tissue Homogenate 5000-78.13 pg/mL 5000 pg/mL Log in to see
0.0012121415 ABIN628222 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Log in to see
0.0012121415 ABIN1380271 Cell ELISA Log in to see
0.0012121415 ABIN512101 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012121415 ABIN1381552 Cell ELISA Log in to see
0.0012121415 ABIN2042672 Sandwich ELISA 5-8000 pg/mL 5 pg/mL Log in to see
0.0012121415 ABIN2115137 Cell ELISA Log in to see
0.0012121415 ABIN2685579 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum, Tissue Homogenate 125-8000 pg/mL 125 pg/mL Log in to see
0.0012121415 ABIN2115136 Cell ELISA Log in to see
0.0012121415 ABIN456731 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012121415 ABIN2685578 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
0.0012121415 ABIN2539400 Sandwich ELISA Serum, Plasma, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
0.0012121415 ABIN2107534 Sandwich ELISA Log in to see
0.0012121415 ABIN2945234 0.156-10 ng/mL 0.156 ng/mL Log in to see

Top referenced TEK ELISA Kit for ELISA

Similar TEK ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 18 ELISA Kits

General

Antigen TEK Tyrosine Kinase, Endothelial (TEK) ELISA Kits
Epitope AA 23-744
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(29), (17), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 125-8000 pg/mL
Minimum Detection Limit 125 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details TEK ELISA Kit

Target details Application Details Handling References for TEK Kit (ABIN1672877) Images
Purpose For quantitative detection of mouse TEK in cell culture supernates, tissue homogenates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse TEK
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse TEK antibod - 1
  • Lyophilized recombinant mouse TEK standar - 10ng/tubex2
  • Biotinylated anti- mouse TEK antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A23-K744

Target details

Product Details TEK ELISA Kit Application Details Handling References for TEK Kit (ABIN1672877) Images back to top
Antigen
Alternative Name TEK (TEK ELISA Kit Abstract)
Background Angiopoietin-1 receptor, also called TEK or TIE2 is a protein that in humans is encoded by the TEK gene. This gene is mapped to 9p21.2. The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations, the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
Gene ID 21687
UniProt Q02858
Pathways RTK Signaling

Application Details

Product Details TEK ELISA Kit Target details Handling References for TEK Kit (ABIN1672877) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse TEK ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Monoclonal antibody from rat specific for TEK has been precoated onto 96-well plates. Standards(NSO, A23-K744) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TEK is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TEK amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse TEK standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, tissue homogenates or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TEK standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 454, Standard deviation: 30.9, CV(%): 6.8
  • Sample 2: n=16, Mean(pg/ml): 1225, Standard deviation: 77.2, CV(%): 6.3
  • Sample 3: n=16, Mean(pg/ml): 4837, Standard deviation: 285.4, CV(%): 5.9,
  • Sample 1: n=24, Mean(pg/ml): 632, Standard deviation: 46.14, CV(%): 7.3
  • Sample 2: n=24, Mean(pg/ml): 1586, Standard deviation: 103.1, CV(%): 6.5
  • Sample 3: n=24, Mean(pg/ml): 5124, Standard deviation: 312.6, CV(%): 6.1
Restrictions For Research Use only

Handling

Product Details TEK ELISA Kit Target details Application Details References for TEK Kit (ABIN1672877) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for TEK Kit (ABIN1672877)

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Background publications

Limaye, Wouters, Uebelhoer, Tuominen, Wirkkala, Mulliken, Eklund, Boon, Vikkula: "Somatic mutations in angiopoietin receptor gene TEK cause solitary and multiple sporadic venous malformations." in: Nature genetics, Vol. 41, Issue 1, pp. 118-24, 2008

Fukuhara, Sako, Minami, Noda, Kim, Kodama, Shibuya, Takakura, Koh, Mochizuki: "Differential function of Tie2 at cell-cell contacts and cell-substratum contacts regulated by angiopoietin-1." in: Nature cell biology, Vol. 10, Issue 5, pp. 513-26, 2008

Arai, Hirao, Ohmura, Sato, Matsuoka, Takubo, Ito, Koh, Suda: "Tie2/angiopoietin-1 signaling regulates hematopoietic stem cell quiescence in the bone marrow niche." in: Cell, Vol. 118, Issue 2, pp. 149-61, 2004

Images

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Supplier Images
ELISA image for TEK ELISA Kit (TEK Tyrosine Kinase, Endothelial) (ABIN1672877) Mouse TEK/TIE2 PicoKine ELISA Kit standard curve