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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||20 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Functional Studies (Func)||11 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||42 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||46 Antibodies|
|Immunoprecipitation (IP)||11 Antibodies|
|Antigen||Vascular Endothelial Growth Factor A (VEGFA) Antibodies|
|Conjugate||This VEGF antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-VEGF AntibodyTarget Details VEGF Application Details Handling Images
|Purification||Tissue culture supernatant|
|Immunogen||A synthetic peptide corresponding to residues on the C-terminus of human VEGF was used as an immunogen.|
Target Details VEGFProduct Details anti-VEGF Antibody Application Details Handling Images back to top
|Alternative Name||VEGF-A (VEGFA Antibody Abstract)|
Vascular endothelial growth factor (VEGF) was recently identified as a secreted,direct-acting mitogen specific for vascular endothelial cells and capable of stimulatingangiogenesis in vivo. Molecular cloning revealed multiple forms of VEGF, apparentlyarising from alternative splicing of its RNA transcript (1). VEGF and TGF-beta1 arepotent angiogenesis inducers with opposing effects on endothelial cells. TGF-beta1induces apoptosis, VEGF protects endothelial cells from apoptosis. We found thatTGF-beta1 promotes endothelial cell expression of FGF-2, which up-regulates VEGFsynthesis. Inhibition of VEGF signaling through VEGF receptor 2 (flk-1) abrogatesTGF-beta1-induced apoptosis and p38(MAPK) activation. This finding illustrates anunexpected role of VEGF and indicates that VEGF can be pharmacologically convertedinto an apoptotic factor, a novel approach to antiangiogenesis therapy (2). Alternate Names: anti-MGC70609 antibody, anti-Vascular Endothelial Growth Factor antibody,anti-Vascular Permeability Factor antibody, anti-VEGF 121 antibody, anti-VEGFAantibody, anti-VPF antibody.
Gene Symbol: VEGFA
|Gene ID||7422, 83785|
|Research Area||Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling|
Application DetailsProduct Details anti-VEGF Antibody Target Details VEGF Handling Images back to top
In Western blot this antibody detects a band at approximately 22 kDa.
Recommended dilutions: Flow Cytometry 1:25, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:50-100, Western Blot 1:1000-10000
Protocol specific for VEGF Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-VEGF Antibody Target Details VEGF Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-VEGF Antibody Target Details VEGF Application Details Handling back to top