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anti-Human VEGF antibody for Immunocytochemistry

Recommended VEGF Antibody (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) Antibodies
  • eVEGF120
  • eVEGF164
  • MVCD1
  • vefg
  • VEGF
  • Vegf
  • vegf
  • VEGF-A
  • vegf-a
  • Vegf120
  • Vegf164
  • VEGF164
  • Vegf188
  • vegfa
  • Vpf
  • VPF
  • vpf
  • wu:fj82c06
Epitope
C-Term
27
24
17
13
13
11
10
9
9
7
6
6
6
5
5
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
264
99
94
25
19
12
11
6
2
1
1
Host
Rabbit
270
71
22
4
2
1
1
Clonality
Monoclonal
Conjugate
This VEGF antibody is un-conjugated
45
26
15
5
5
5
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
264
201
66
59
24
22
15
14
13
13
10
6
3
2
2
1
Supplier
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Available images

Catalog No. ABIN258536
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN2506673 ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN3201330 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 28-191 Log in to see
0.0009962841 ABIN1078646 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 208-327 Log in to see Polyclonal

Similar anti-VEGF Antibodies

Application / Reactivity Human
ELISA 132 Antibodies
Enzyme Immunoassay (EIA) 20 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Functional Studies (Func) 11 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 42 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies
Immunoprecipitation (IP) 11 Antibodies

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) Antibodies
Epitope C-Term
(27), (24), (17), (13), (13), (11), (10), (9), (9), (7), (6), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(264), (99), (94), (25), (19), (12), (11), (6), (2), (1), (1)
Host Rabbit
(270), (71), (22), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This VEGF antibody is un-conjugated
(45), (26), (15), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(264), (201), (66), (59), (24), (22), (15), (14), (13), (13), (10), (6), (3), (2), (2), (1)
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Product Details anti-VEGF Antibody

Target Details VEGF Application Details Handling Images
Purification Tissue culture supernatant
Immunogen A synthetic peptide corresponding to residues on the C-terminus of human VEGF was used as an immunogen.
Clone EP1176Y
Isotype IgG

Target Details VEGF

Product Details anti-VEGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name VEGF-A (VEGFA Antibody Abstract)
Background Vascular endothelial growth factor (VEGF) was recently identified as a secreted,direct-acting mitogen specific for vascular endothelial cells and capable of stimulatingangiogenesis in vivo. Molecular cloning revealed multiple forms of VEGF, apparentlyarising from alternative splicing of its RNA transcript (1). VEGF and TGF-beta1 arepotent angiogenesis inducers with opposing effects on endothelial cells. TGF-beta1induces apoptosis, VEGF protects endothelial cells from apoptosis. We found thatTGF-beta1 promotes endothelial cell expression of FGF-2, which up-regulates VEGFsynthesis. Inhibition of VEGF signaling through VEGF receptor 2 (flk-1) abrogatesTGF-beta1-induced apoptosis and p38(MAPK) activation. This finding illustrates anunexpected role of VEGF and indicates that VEGF can be pharmacologically convertedinto an apoptotic factor, a novel approach to antiangiogenesis therapy (2). Alternate Names: anti-MGC70609 antibody, anti-Vascular Endothelial Growth Factor antibody,anti-Vascular Permeability Factor antibody, anti-VEGF 121 antibody, anti-VEGFAantibody, anti-VPF antibody.
Gene Symbol: VEGFA
Gene ID 7422, 83785
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling
Pathways RTK Signaling

Application Details

Product Details anti-VEGF Antibody Target Details VEGF Handling Images back to top
Application Notes In Western blot this antibody detects a band at approximately 22 kDa.
Recommended dilutions: Flow Cytometry 1:25, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:50-100, Western Blot 1:1000-10000
Protocol Protocol specific for VEGF Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-VEGF Antibody Target Details VEGF Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-VEGF antibody (Vascular Endothelial Growth Factor A) (C-Term) (ABIN258536) Western blot analysis on 5 ng of recombinant protein using anti-VEGF RabMAb (cat. # 1...
Immunohistochemistry (IHC) image for anti-VEGF antibody (Vascular Endothelial Growth Factor A) (C-Term) (ABIN258536) Immunohistochemical staining of hemangioma using anti-VEGF RabMAb (cat. #110-57642).