VEGFA antibody (AA 141-180)

Catalog No. ABIN707186

Product Details anti-VEGFA Antibody

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Binding Specificity: AA 141-180

Reactivity: Human, Rat, Mouse, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Chicken, Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human VEGF-A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-VEGFA antibody (ABIN707186)

Fetoni, Eramo, Paciello, Rolesi, Samengo, Paludetti, Troiani, Pani: "The redox protein p66(shc) mediates cochlear vascular dysfunction and transient noise-induced hearing loss." in: Scientific reports, Vol. 6, pp. 25450, (2016) (PubMed).

Shi, Ko, Ko, Kim, Ko: "Peptide Lv augments L-type voltage-gated calcium channels through vascular endothelial growth factor receptor 2 (VEGFR2) signaling." in: Biochimica et biophysica acta, Vol. 1853, Issue 5, pp. 1154-64, (2015) (PubMed).

Wang, Liao, Wang, Deng, Yu: "Transplantation of bone marrow stromal cells overexpressing human vascular endothelial growth factor 165 enhances tissue repair in a rat model of radiation-induced injury." in: Chinese medical journal, Vol. 127, Issue 6, pp. 1093-9, (2014) (PubMed).

Fetoni, Lattanzi, Eramo, Barba, Paciello, Moriconi, Rolesi, Michetti, Troiani, Paludetti: "Grafting and early expression of growth factors from adipose-derived stem cells transplanted into the cochlea, in a Guinea pig model of acoustic trauma." in: Frontiers in cellular neuroscience, Vol. 8, pp. 334, (2014) (PubMed).

Tian, Yang, Xiao, Huang, He, Ma, Xia, Shi: "Mechanisms of neuroprotection from hypoxia-ischemia (HI) brain injury by up-regulation of cytoglobin (CYGB) in a neonatal rat model." in: The Journal of biological chemistry, Vol. 288, Issue 22, pp. 15988-6003, (2013) (PubMed).

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGF-A (VEGFA Products)

Background:

Synonyms: VPF, VEGF, MVCD1, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VEGFA

Background: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Gene ID: 7422

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling