anti-Mouse (Murine) VEGF antibody for Immunohistochemistry (Frozen Sections)

Recommended VEGF Antibody (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) Antibodies
  • vegf
  • vegfa
  • wu:fj82c06
  • VEGF
  • vegf-a
  • vpf
  • vefg
  • VEGF-A
  • VPF
  • eVEGF120
  • eVEGF164
  • MVCD1
  • Vegf
  • Vegf120
  • Vegf164
  • Vegf188
  • Vpf
  • VEGF164
  • vascular endothelial growth factor Aa
  • vascular endothelial growth factor A
  • vegfaa
  • vegfa-a
  • VEGFA
  • vegfa
  • Vegfa
Epitope
AA 27-190
24
19
18
15
15
12
10
10
10
9
7
6
6
6
6
6
5
5
5
5
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
305
116
109
29
24
16
13
8
3
3
3
2
1
1
1
1
1
1
Host
Rabbit
271
99
24
4
2
1
1
Clonality
Polyclonal
Conjugate
This VEGF antibody is un-conjugated
43
24
13
7
6
5
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
296
196
81
57
26
26
26
18
18
16
11
10
7
4
4
2
2
2
1
1
Options
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Catalog No. ABIN1078647
$ 232.00
Plus shipping costs $45.00

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) Antibodies
Epitope AA 27-190
(24), (19), (18), (15), (15), (12), (10), (10), (10), (9), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(305), (116), (109), (29), (24), (16), (13), (8), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(271), (99), (24), (4), (2), (1), (1)
Clonality
Polyclonal
Conjugate This VEGF antibody is un-conjugated
(43), (24), (13), (7), (6), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(296), (196), (81), (57), (26), (26), (26), (18), (18), (16), (11), (10), (7), (4), (4), (2), (2), (2), (1), (1)
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Product Details anti-VEGF Antibody

Target Details VEGF Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- APTT EGEQKSHEVI KFMDVYQRSY CRPIETLVDI FQEYPDEIEY IFKPSCVPLM RCAGCCNDEA LECVPTSESN ITMQIMRIKP HQSQHIGEMS FLQHSRCECR PKKDRTKPEK KSVRGKGKGQ KRKRKKSRFK SWSVHCEPCS ERRKHLFVQD PQTCKCSCKN
Specificity It has been selected for its ability to recognize VEGFA in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen Recombinant VEGFA expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against VEGFA.
Isotype IgG

Target Details VEGF

Product Details anti-VEGF Antibody Application Details Handling Images back to top
Antigen
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-VEGF Antibody Target Details VEGF Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant VEGFA (Ala27~Asn190) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-VEGF Antibody Target Details VEGF Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) antibody (ABIN1078647) anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) antibody