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anti-Rat (Rattus) VEGF antibody for Enzyme Immunoassay

Recommended VEGF Antibody (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) Antibodies
  • eVEGF120
  • eVEGF164
  • MVCD1
  • vefg
  • VEGF
  • Vegf
  • vegf
  • VEGF-A
  • vegf-a
  • Vegf120
  • Vegf164
  • VEGF164
  • Vegf188
  • vegfa
  • Vpf
  • VPF
  • vpf
  • wu:fj82c06
Epitope
N-Term
26
24
17
13
13
11
10
9
9
7
6
6
6
5
5
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
263
98
93
25
19
12
11
6
2
1
1
Host
Rabbit
269
71
22
4
2
1
1
Clonality
Polyclonal
Conjugate
This VEGF antibody is un-conjugated
45
26
15
5
5
5
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
263
201
66
59
23
22
15
14
13
13
10
6
3
2
2
1
Supplier
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Catalog No. ABIN115675
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088587624 ABIN115674 EIA WB Rabbit N-Term Log in to see Polyclonal

Similar anti-VEGF Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 196 Antibodies 57 Antibodies 63 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 13 Antibodies 1 Antibodies 1 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Immunoprecipitation (IP) 11 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies 19 Antibodies 15 Antibodies
Immunohistochemistry (IHC) 42 Antibodies 17 Antibodies 35 Antibodies

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) Antibodies
Epitope N-Term
(26), (24), (17), (13), (13), (11), (10), (9), (9), (7), (6), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(263), (98), (93), (25), (19), (12), (11), (6), (2), (1), (1)
Host Rabbit
(269), (71), (22), (4), (2), (1), (1)
Clonality
Polyclonal
Conjugate This VEGF antibody is un-conjugated
(45), (26), (15), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(263), (201), (66), (59), (23), (22), (15), (14), (13), (13), (10), (6), (3), (2), (2), (1)
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Product Details anti-VEGF Antibody

Target Details VEGF Application Details Handling Images
Specificity The antibody will react with all VEGF-A isoforms.
Purification Affinity Chromatography with immobilized Protein A
Immunogen Highly purified N-terminal 20 amino acid peptide of Human VEGF-A

Target Details VEGF

Product Details anti-VEGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name VEGF-A (VEGFA Antibody Abstract)
Background VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Gene ID 7422
NCBI Accession NP_001020537
UniProt P15692
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling
Pathways RTK Signaling

Application Details

Product Details anti-VEGF Antibody Target Details VEGF Handling Images back to top
Application Notes ELISA: To detect Human VEGF by Direct ELISA a concentration of at least 0.5-2 μg/mL isrequired. This purified IgG, in combination with compatible secondary reagents, allows thedetection of 1,0- 2,5 ng/well of recombinant Human VEGF165 or other VEGF splice forms. Western Blot: 5-10 μg/mL. It will detect 25 ng/lane of recombinant human and murineVEGF-A under reducing conditions, dimers are detected at higher protein concentrations.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-VEGF Antibody Target Details VEGF Application Details Images back to top
Reconstitution Restore in sterile water to a concentration of > 0.5 mg/mL
Buffer PBS, pH 7.4 containing no preservative
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment The lyophilized IgG is stable at 2-8 °C for at least one month and for longer when kept at -20 °C. When reconstituted the antibody is stable for at least six weeks at 2-4 °C.