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anti-Hamster VEGFR2 antibody for Western Blotting

Recommended VEGFR2 Antibody (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
  • vegfr-2
  • KDR
  • 6130401C07
  • Flk-1
  • Flk1
  • Krd-1
  • Ly73
  • VEGFR-2
  • VEGFR2
  • sVEGFR-2
  • Vegfr-2
  • CD309
  • FLK1
  • VEGFR
  • FLK-1
  • flk-1
  • FLK/KDR
  • flk1
  • flk1b
  • kdrb
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • wu:fc31a09
  • fk52c05
  • flk
  • kdr
  • kdra
  • vegfr2
  • vegfr4
  • vegr2
  • wu:fk52c05
  • vascular endothelial growth factor receptor 2
  • kinase insert domain protein receptor
  • kinase insert domain receptor
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • kinase insert domain receptor like
  • vascular endothelial growth factor receptor-2
  • vegfr-2
  • Kdr
  • KDR
  • kdr
  • kdrl
Epitope
AA 1300-1367, C-Term, Native Form
76
57
52
37
25
23
21
18
18
17
16
16
15
15
13
13
13
12
12
11
10
10
9
7
6
6
6
5
5
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Hamster, Human
601
357
182
25
7
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
512
139
60
4
2
2
1
Clonality
Polyclonal
Conjugate
This VEGFR2 antibody is un-conjugated
43
32
24
20
16
12
11
9
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
444
235
208
198
111
101
40
34
26
24
19
17
11
9
8
7
6
5
3
2
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN250774
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1582155 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 1
1 ABIN407044 FACS ICC IF WB DyLight 488 Rabbit AA 1300-1367, C-Term, Native Form Log in to see Polyclonal 2

Top referenced anti-VEGFR2 antibody for Western Blotting

Similar anti-VEGFR2 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 3 Antibodies
Dot Blot (DB) 17 Antibodies
ELISA 197 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 19 Antibodies
Flow Cytometry (FACS) 146 Antibodies
Functional Studies (Func) 6 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 36 Antibodies
Immunofluorescence (IF) 110 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 185 Antibodies

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
Epitope AA 1300-1367, C-Term, Native Form
(76), (57), (52), (37), (25), (23), (21), (18), (18), (17), (16), (16), (15), (15), (13), (13), (13), (12), (12), (11), (10), (10), (9), (7), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Hamster, Human
(601), (357), (182), (25), (7), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(512), (139), (60), (4), (2), (2), (1)
Clonality
Polyclonal
Conjugate This VEGFR2 antibody is un-conjugated
(43), (32), (24), (20), (16), (12), (11), (9), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(444), (235), (208), (198), (111), (101), (40), (34), (26), (24), (19), (17), (11), (9), (8), (7), (6), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-VEGFR2 Antibody

Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 Antibody (ABIN250774) Images
Specificity This has only been shown to recognize the native form (180-190 kDa protein) of VEGFR-2, known as Flk-1/ KDR/ VEGFR 2 (Vascular Endothelial Growth Factor Receptor 2), though it was raised against the precursor form.
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to a C-terminal region of the mouse VEGF Receptor 2 protein (between residues 1300-1367). [Swiss-Prot# P35918]

Target Details VEGFR2

Product Details anti-VEGFR2 Antibody Application Details Handling References for anti-VEGFR2 Antibody (ABIN250774) Images back to top
Antigen
Alternative Name CD309 / VEGFR-2 / Flk-1 (KDR Antibody Abstract)
Background Gene Symbol: KDR
Molecular Weight Theoretical MW: 150 kDa
Gene ID 3791
UniProt P35918
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling References for anti-VEGFR2 Antibody (ABIN250774) Images back to top
Application Notes Western Blot 1 μg/mL, Immunocytochemistry/Immunofluorescence 1:10This VEGF Receptor 2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a doublet is seen at ~150 kDa with CSF-1R/VEGFR2 chimera transfected lysates representing VEGFR-2. A non-specific band is also seen at ~85 kDa. In ICC/IF punctate membrane staining was observed in Hela cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot protocol specific for VEGF Receptor 2 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS overnight at 4 °C.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-VEGFR2 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details References for anti-VEGFR2 Antibody (ABIN250774) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-VEGFR2 Antibody (ABIN250774)

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling Images back to top
Product cited in:

Zhou, Rogers, Lee, Sun, Yao, Mao, Kong: "Oral Mucosa Harbors a High Frequency of Endothelial Cells: A Novel Postnatal Cell Source for Angiogenic Regeneration." in: Stem cells and development, Vol. 26, Issue 2, pp. 91-101, 2016 Method employed by authors: Flow Cytometry (FACS) (Sample species: Rat (Rattus)).

Lim, Yap, Lim, Goh, Ng: "Identification of autocrine growth factors secreted by CHO cells for applications in single-cell cloning media." in: Journal of proteome research, Vol. 12, Issue 7, pp. 3496-510, 2013 (Sample species: Hamster). Further details: Western Blotting

Roan, Fang, Chang, Hsu, Huang, Chiou, Tsai, Lam: "Rosuvastatin improves vascular function of arteriovenous fistula in a diabetic rat model." in: Journal of vascular surgery, Vol. 56, Issue 5, pp. 1381-9.e1, 2012 (Sample species: Rat (Rattus)). Further details: Flow Cytometry

Hu-Lowe, Zou, Grazzini, Hallin, Wickman, Amundson, Chen, Rewolinski, Yamazaki, Wu, McTigue, Murray, Kania, OConnor, Shalinsky, Bender: "Nonclinical antiangiogenesis and antitumor activities of axitinib (AG-013736), an oral, potent, and selective inhibitor of vascular endothelial growth factor receptor tyrosine kinases 1, 2, 3." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 22, pp. 7272-83, 2008

Takahashi, Hattori, Iwamatsu, Takizawa, Shibuya: "A novel snake venom vascular endothelial growth factor (VEGF) predominantly induces vascular permeability through preferential signaling via VEGF receptor-1." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 46304-14, 2004

Rahimi, Dayanir, Lashkari: "Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16986-92, 2000

Background publications

Gluzman-Poltorak, Cohen, Shibuya, Neufeld: "Vascular endothelial growth factor receptor-1 and neuropilin-2 form complexes." in: The Journal of biological chemistry, Vol. 276, Issue 22, pp. 18688-94, 2001

Images

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 Antibody (ABIN250774) back to top
Supplier Images
Western Blotting (WB) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (AA 1300-1367) (ABIN250774) Detection of VEGFR2 doublet in CSF-IR/VEGFR2 chimera transfected lysate using ABIN250...
Immunofluorescence (IF) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (AA 1300-1367) (ABIN250774) Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody [ABIN250774] - VEG...