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anti-Human VEGFR2 antibody for Immunocytochemistry

Recommended VEGFR2 Antibody (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
  • vegfr-2
  • KDR
  • 6130401C07
  • Flk-1
  • Flk1
  • Krd-1
  • Ly73
  • VEGFR-2
  • VEGFR2
  • sVEGFR-2
  • Vegfr-2
  • CD309
  • FLK1
  • VEGFR
  • FLK-1
  • flk-1
  • FLK/KDR
  • flk1
  • flk1b
  • kdrb
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • wu:fc31a09
  • fk52c05
  • flk
  • kdr
  • kdra
  • vegfr2
  • vegfr4
  • vegr2
  • wu:fk52c05
  • vascular endothelial growth factor receptor 2
  • kinase insert domain protein receptor
  • kinase insert domain receptor
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • kinase insert domain receptor like
  • vascular endothelial growth factor receptor-2
  • vegfr-2
  • Kdr
  • KDR
  • kdr
  • kdrl
Epitope
AA 20-764
76
57
52
37
24
23
21
18
18
18
16
16
15
15
13
13
13
12
12
11
10
10
9
7
6
6
5
5
5
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
597
354
182
23
7
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
Host
Mouse
510
137
57
4
2
2
1
Clonality
Monoclonal
Conjugate
This VEGFR2 antibody is un-conjugated
42
32
24
20
16
12
11
9
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
439
234
208
190
110
100
39
34
26
24
17
16
11
9
8
7
6
5
3
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN969231
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.253727 ABIN4900629 FACS ICC WB Rabbit IgG pTyr1214 Log in to see Polyclonal 2
26.253727 ABIN3031551 ICC IHC (fro) IHC (p) WB Rabbit IgG Middle Region Log in to see Polyclonal
1 ABIN250774 ICC IF WB Rabbit AA 1300-1367, C-Term, Native Form Log in to see Polyclonal 7
1 ABIN152343 CyTOF ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see EIC 1
1 ABIN2869451 ELISA FACS ICC WB Mouse IgG1 AA 20-764 Log in to see 4B4 3
1 ABIN3044301 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 454-469, Middle Region Log in to see Polyclonal
1 ABIN407044 FACS ICC IF WB DyLight 488 Rabbit AA 1300-1367, C-Term, Native Form Log in to see Polyclonal 2
1 ABIN257630 ICC IF IHC (p) IHC WB Rabbit pTyr951 Log in to see Polyclonal
1 ABIN151961 ICC IF WB Rabbit Internal Region, AA 1200-1300 Log in to see Polyclonal
1 ABIN958010 ICC FACS WB Rabbit IgG Tyr1214 Log in to see Polyclonal
1 ABIN151962 ICC IF WB Rabbit C-Term, AA 1300-1367 Log in to see Polyclonal 1
1 ABIN1873397 FACS ICC IHC WB Mouse IgG Log in to see
1 ABIN2506667 ICC IF IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN446800 ICC IF IHC (p) IHC Rabbit Log in to see Polyclonal
1 ABIN449648 ICC IHC (fro) IF IHC ELISA WB Mouse IgG1 Log in to see 20I6
1 ABIN1078658 ICC IHC IP WB Rabbit IgG AA 46-320 Log in to see Polyclonal
1 ABIN222793 ICC IF ELISA WB Rabbit IgG pTyr1175 Log in to see
1 ABIN2624471 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 454-469 Log in to see Polyclonal
1 ABIN1825715 ICC IHC (p) WB Rabbit Tyr1175 Log in to see Polyclonal
1 ABIN1825716 ICC IHC (p) WB Rabbit pTyr1214 Log in to see Polyclonal

Top referenced anti-VEGFR2 antibody for Immunocytochemistry

Similar anti-VEGFR2 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 3 Antibodies
Dot Blot (DB) 17 Antibodies
ELISA 197 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 16 Antibodies
Flow Cytometry (FACS) 142 Antibodies
Functional Studies (Func) 6 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 36 Antibodies
Immunofluorescence (IF) 109 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 185 Antibodies

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
Epitope AA 20-764
(76), (57), (52), (37), (24), (23), (21), (18), (18), (18), (16), (16), (15), (15), (13), (13), (13), (12), (12), (11), (10), (10), (9), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(597), (354), (182), (23), (7), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(510), (137), (57), (4), (2), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This VEGFR2 antibody is un-conjugated
(42), (32), (24), (20), (16), (12), (11), (9), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(439), (234), (208), (190), (110), (100), (39), (34), (26), (24), (17), (16), (11), (9), (8), (7), (6), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-VEGFR2 Antibody

Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 Antibody (ABIN969231) Images
Purification Ascites
Immunogen Purified recombinant extracellular fragment of human KDR (AA 20-764) fused with hIgGFc tag expressed in HEK293 cells.
Clone 4B4
Isotype IgG1

Target Details VEGFR2

Product Details anti-VEGFR2 Antibody Application Details Handling References for anti-VEGFR2 Antibody (ABIN969231) Images back to top
Antigen
Alternative Name KDR (KDR Antibody Abstract)
Background KDR has also been designated as VEFR-2 (Vascular endothelial growth factor receptor 2), CD309 (cluster of differentiation 309) and Flk1 (fetal liver kinase 1). Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. KDR is one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
Molecular Weight 152 kDa
Gene ID 3791
HGNC 3791
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling References for anti-VEGFR2 Antibody (ABIN969231) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details References for anti-VEGFR2 Antibody (ABIN969231) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-VEGFR2 Antibody (ABIN969231)

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling Images back to top
Background publications

Lee, Bone, Strege, Shanafelt, Jelinek, Kay: "VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia." in: Blood, Vol. 104, Issue 3, pp. 788-94, 2004

Product cited in:

Takahashi, Yamaguchi, Chida, Shibuya: "A single autophosphorylation site on KDR/Flk-1 is essential for VEGF-A-dependent activation of PLC-gamma and DNA synthesis in vascular endothelial cells." in: The EMBO journal, Vol. 20, Issue 11, pp. 2768-78, 2001

Images

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 Antibody (ABIN969231) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (AA 20-764) (ABIN969231) Figure 1: Flow cytometric analysis of HepG2 cells using KDR mouse mAb (green) and neg...
Immunocytochemistry (ICC) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (AA 20-764) (ABIN969231) Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells...
Western Blotting (WB) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (AA 20-764) (ABIN969231) Figure 3: Western blot analysis using KDR mouse mAb against extracellular domain of h...