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anti-Human VEGFR2 antibody for Immunocytochemistry

Recommended VEGFR2 Antibody (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
  • 6130401C07
  • CD309
  • fk52c05
  • flk
  • FLK-1
  • flk-1
  • Flk-1
  • FLK/KDR
  • Flk1
  • flk1
  • FLK1
  • flk1b
  • kdr
  • KDR
  • kdra
  • kdrb
  • Krd-1
  • Ly73
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • sVEGFR-2
  • VEGFR
  • Vegfr-2
  • vegfr-2
  • VEGFR-2
  • VEGFR2
  • vegfr2
  • vegfr4
  • vegr2
  • wu:fc31a09
  • wu:fk52c05
Epitope
AA 20-764
79
52
50
36
24
20
18
18
17
17
14
14
13
13
12
12
11
10
9
7
6
6
6
6
5
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
536
274
150
5
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Host
Mouse
483
106
51
2
2
2
1
Clonality
Monoclonal
Conjugate
This VEGFR2 antibody is un-conjugated
38
25
22
16
14
12
9
7
5
4
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
401
230
204
125
104
79
31
27
23
22
17
16
8
6
6
5
5
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN969231
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.67686415 ABIN1846703 ICC FACS IF ELISA WB Mouse IgG1 AA 20-764 Log in to see
0.00099893 ABIN250774 ICC IF WB Rabbit AA 1300-1367, C-Term, Native Form Log in to see Polyclonal 6
0.00099893 ABIN152343 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see EIC 1
0.00099893 ABIN2869451 ELISA FACS ICC WB Mouse IgG1 AA 20-764 Log in to see 4B4 3
0.00099893 ABIN3044301 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 454-469, Middle Region Log in to see Polyclonal
0.00099893 ABIN958010 ICC FACS WB Rabbit IgG Tyr1214 Log in to see Polyclonal
0.00099893 ABIN222793 ICC IF ELISA WB Rabbit IgG pTyr1175 Log in to see
0.00099893 ABIN151962 ICC IF WB Rabbit C-Term, AA 1300-1367 Log in to see Polyclonal
0.00099893 ABIN257630 ICC IF IHC (p) IHC WB Rabbit IgG pTyr951 Log in to see Polyclonal
0.00099893 ABIN1873397 ICC FACS WB Mouse Log in to see
0.00099893 ABIN151961 ICC IF WB Rabbit Internal Region, AA 1200-1300 Log in to see Polyclonal
0.00099893 ABIN2506667 ICC IF IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
0.00099893 ABIN1907753 ICC FACS IHC ELISA WB APC Rabbit IgG C-Term, AA 1327-1356 Log in to see Polyclonal
0.00099893 ABIN1907755 ICC FACS IHC ELISA WB PE Rabbit IgG C-Term, AA 1327-1356 Log in to see Polyclonal
0.00099893 ABIN1907754 ICC FACS IHC ELISA WB FITC Rabbit IgG C-Term, AA 1327-1356 Log in to see Polyclonal
0.00099893 ABIN1907752 ICC FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 1327-1356 Log in to see Polyclonal
0.00099893 ABIN1078658 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 46-320 Log in to see Polyclonal
0.00099893 ABIN2624471 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 454-469 Log in to see Polyclonal
0.00099893 ABIN1825539 ICC IHC ELISA WB Mouse IgG1 Log in to see
0.00099893 ABIN1825716 ICC IHC WB Rabbit pTyr1214 Log in to see Polyclonal

Top referenced anti-VEGFR2 antibody for Immunocytochemistry

Similar anti-VEGFR2 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
Dot Blot (DB) 17 Antibodies
ELISA 198 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 16 Antibodies
Flow Cytometry (FACS) 96 Antibodies
Functional Studies (Func) 6 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 36 Antibodies
Immunofluorescence (IF) 109 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 197 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 80 Antibodies

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
Epitope AA 20-764
(79), (52), (50), (36), (24), (20), (18), (18), (17), (17), (14), (14), (13), (13), (12), (12), (11), (10), (9), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(536), (274), (150), (5), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(483), (106), (51), (2), (2), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This VEGFR2 antibody is un-conjugated
(38), (25), (22), (16), (14), (12), (9), (7), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(401), (230), (204), (125), (104), (79), (31), (27), (23), (22), (17), (16), (8), (6), (6), (5), (5), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-VEGFR2 Antibody

Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 Antibody (ABIN969231) Images
Purification Ascites
Immunogen Purified recombinant extracellular fragment of human KDR (AA 20-764) fused with hIgGFc tag expressed in HEK293 cells.
Clone 4B4
Isotype IgG1

Target Details VEGFR2

Product Details anti-VEGFR2 Antibody Application Details Handling References for anti-VEGFR2 Antibody (ABIN969231) Images back to top
Antigen
Alternative Name KDR (KDR Antibody Abstract)
Background KDR has also been designated as VEFR-2 (Vascular endothelial growth factor receptor 2), CD309 (cluster of differentiation 309) and Flk1 (fetal liver kinase 1). Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. KDR is one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
Molecular Weight 152 kDa
Gene ID 3791
HGNC 3791
Pathways RTK Signaling

Application Details

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling References for anti-VEGFR2 Antibody (ABIN969231) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details References for anti-VEGFR2 Antibody (ABIN969231) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-VEGFR2 Antibody (ABIN969231)

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling Images back to top
Background publications

Lee, Bone, Strege, Shanafelt, Jelinek, Kay: "VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia." in: Blood, Vol. 104, Issue 3, pp. 788-94, 2004

Product cited in:

Takahashi, Yamaguchi, Chida, Shibuya: "A single autophosphorylation site on KDR/Flk-1 is essential for VEGF-A-dependent activation of PLC-gamma and DNA synthesis in vascular endothelial cells." in: The EMBO journal, Vol. 20, Issue 11, pp. 2768-78, 2001

Images

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 Antibody (ABIN969231) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (AA 20-764) (ABIN969231) Figure 1: Flow cytometric analysis of HepG2 cells using KDR mouse mAb (green) and neg...
Immunocytochemistry (ICC) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (AA 20-764) (ABIN969231) Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells...
Western Blotting (WB) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (AA 20-764) (ABIN969231) Figure 3: Western blot analysis using KDR mouse mAb against extracellular domain of h...