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anti-Mouse (Murine) VEGFR2 antibody for Immunocytochemistry

Recommended VEGFR2 Antibody (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
  • 6130401C07
  • CD309
  • fk52c05
  • flk
  • FLK-1
  • flk-1
  • Flk-1
  • FLK/KDR
  • Flk1
  • flk1
  • FLK1
  • flk1b
  • kdr
  • KDR
  • kdra
  • kdrb
  • Krd-1
  • Ly73
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • sVEGFR-2
  • VEGFR
  • Vegfr-2
  • vegfr-2
  • VEGFR-2
  • VEGFR2
  • vegfr2
  • vegfr4
  • vegr2
  • wu:fc31a09
  • wu:fk52c05
Epitope
C-Term, AA 1300-1367
79
52
50
36
24
20
18
18
17
17
14
14
13
13
12
12
12
10
9
7
6
6
6
6
5
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
538
275
151
5
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
484
107
51
2
2
2
1
Clonality
Polyclonal
Conjugate
This VEGFR2 antibody is un-conjugated
38
25
22
16
14
12
9
7
5
4
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
403
231
205
126
105
79
31
27
23
22
17
16
8
6
6
5
5
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN251662
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009957016 ABIN257628 ICC IF IHC (p) IHC Rabbit IgG Tyr1175 Log in to see Polyclonal
0.0009957016 ABIN251661 ICC IF WB Rabbit Internal Region, AA 1200-1300 Log in to see Polyclonal
0.0009957016 ABIN2506667 ICC IF IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
0.0009957016 ABIN1174537 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 46-322 Log in to see Polyclonal
0.0009957016 ABIN446800 ICC IF IHC (p) IHC Rabbit Log in to see Polyclonal
0.0009957016 ABIN2988108 ICC IF IHC WB Rabbit IgG pTyr1175 Log in to see Polyclonal
0.0009957016 ABIN2988111 ICC IF IHC WB Rabbit IgG pTyr1214 Log in to see Polyclonal
0.0009957016 ABIN2971655 ICC IF IHC WB Rabbit C-Term, pTyr1175 Log in to see Polyclonal
0.0009957016 ABIN3066119 ICC IF IHC WB Rabbit Center, pTyr951 Log in to see Polyclonal

Similar anti-VEGFR2 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Reagent (BR) 2 Antibodies
ELISA 71 Antibodies
ELISA (Detection) 6 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 39 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 55 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 103 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 43 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Intracellular Flow Cytometry (ICFC) 5 Antibodies

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
Epitope C-Term, AA 1300-1367
(79), (52), (50), (36), (24), (20), (18), (18), (17), (17), (14), (14), (13), (13), (12), (12), (12), (10), (9), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(538), (275), (151), (5), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(484), (107), (51), (2), (2), (2), (1)
Clonality
Polyclonal
Conjugate This VEGFR2 antibody is un-conjugated
(38), (25), (22), (16), (14), (12), (9), (7), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(403), (231), (205), (126), (105), (79), (31), (27), (23), (22), (17), (16), (8), (6), (6), (5), (5), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-VEGFR2 Antibody

Target Details VEGFR2 Application Details Handling Images
Cross-Reactivity (Details) Predicted to react with rat,based on 100% sequence homology.
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to a C-terminal region of the mouse VEGF Receptor 2 protein (between residues 1300-1367). [Swiss-Prot# P35918]

Target Details VEGFR2

Product Details anti-VEGFR2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD309 / VEGFR-2 / Flk-1 (KDR Antibody Abstract)
Background Gene Symbol: KDR
Molecular Weight Theoretical MW: 150 kDa
UniProt P35918
Pathways RTK Signaling

Application Details

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling Images back to top
Application Notes Western Blot 1:250-1:1000, Immunocytochemistry/Immunofluorescence 1:50-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot Protocol for VEGF Receptor 2 Antibody
Western Blot Protocol:
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 50 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 0.5 % BSA in TBS for 1 hour.
. Dilute the rabbit anti-VEGFR2 primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.Immunocytochemistry/Immunofluorescence Protocol for VEGF Receptor 2 Antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only

Handling

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Images back to top
Format Liquid
Concentration 2.55 mg/mL
Buffer Tris-glycine, 150 mM NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

Images

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (C-Term) (ABIN251662) Detection of VEGFR-2 doublet in CSF-1 receptor/VEGFR2 chimera transfected lysate (20 ...
Western Blotting (WB) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (C-Term) (ABIN251662) Detection of VEGFR-2 doublet in VEGFR-2 induced HUVEC lysate (50 ug) using ABIN151962...