anti-Human SHANK1 antibody for Western Blotting

Recommended SHANK1 Antibody (supplied by: Log in to see )

Antigen
SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1) Antibodies
  • SPANK-1
  • SSTRIP
  • synamon
  • Shank1a
  • Spank1
  • Sstrip
  • SH3 and multiple ankyrin repeat domains 1
  • SH3/ankyrin domain gene 1
  • SHANK1
  • Shank1
Epitope
AA 469-691
38
15
10
6
3
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
76
74
62
5
2
2
2
1
1
1
1
Host
Mouse
47
43
Clonality
Monoclonal
Conjugate
This SHANK1 antibody is un-conjugated
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Antibody Array (AA), Immunohistochemistry (IHC), Western Blotting (WB)
71
56
26
24
20
17
13
9
7
2
1
Options
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Catalog No. ABIN863122
$ 383.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9060593 ABIN152512 ICC IF IHC (p) IHC WB Rabbit C-Term Log in to see Polyclonal 3
2.9060593 ABIN152511 IHC WB Rabbit N-Term, AA 110-160 Log in to see Polyclonal 1
2.9060593 ABIN1742345 WB Rabbit AA 135-149 Log in to see Polyclonal 1
2.9060593 ABIN2483699 AA IHC WB Atto 390 Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483705 AA IHC WB Atto 680 Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483701 AA IHC WB Atto 565 Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483711 AA IHC WB HRP Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483706 AA IHC WB Atto 700 Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483707 AA IHC WB Alkaline Phosphatase (AP) Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483708 AA IHC WB APC Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483703 AA IHC WB Atto 633 Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483700 AA IHC WB Atto 488 Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483702 AA IHC WB Atto 594 Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483704 AA IHC WB Atto 655 Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483709 AA IHC WB Biotin Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483710 AA IHC WB FITC Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483712 AA IHC WB PE-Atto 594 Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483713 AA IHC WB PerCP Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483714 AA IHC WB PE Mouse IgG1 AA 469-691 Log in to see S22-21
2.9060593 ABIN2483715 AA IHC WB Streptavidin Mouse IgG1 AA 469-691 Log in to see S22-21

General

Antigen SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1) Antibodies
Epitope AA 469-691
(38), (15), (10), (6), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(76), (74), (62), (5), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(47), (43)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SHANK1 antibody is un-conjugated
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Antibody Array (AA), Immunohistochemistry (IHC), Western Blotting (WB)
(71), (56), (26), (24), (20), (17), (13), (9), (7), (2), (1)
Pubmed 1 reference available
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Product Details anti-SHANK1 Antibody

Target Details SHANK1 Application Details Handling References for anti-SHANK1 antibody (ABIN863122) Images
Specificity Detects ~190-220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.
Purification Protein G Purified
Immunogen Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1
Clone S22-21
Isotype IgG1

Target Details SHANK1

Product Details anti-SHANK1 Antibody Application Details Handling References for anti-SHANK1 antibody (ABIN863122) Images back to top
Antigen
Alternative Name SHANK1 (SHANK1 Antibody Abstract)
Background Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina (2). Other recent studies suggest that the disruption of glutamate receptors at the Shank postsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer's disease (3).
Gene ID 78957
NCBI Accession NP_113939
UniProt Q9WV48
Research Area Neurology
Pathways Synaptic Membrane, Maintenance of Protein Location

Application Details

Product Details anti-SHANK1 Antibody Target Details SHANK1 Handling References for anti-SHANK1 antibody (ABIN863122) Images back to top
Application Notes
  • WB (1:1000)
  • IHC (1:1000)
  • ICC/IF (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SMC-329 was sufficient for detection of Shank1 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions For Research Use only

Handling

Product Details anti-SHANK1 Antibody Target Details SHANK1 Application Details References for anti-SHANK1 antibody (ABIN863122) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-SHANK1 antibody (ABIN863122)

Product Details anti-SHANK1 Antibody Target Details SHANK1 Application Details Handling Images back to top
Background publications

Hung, Futai, Sala, Valtschanoff, Ryu, Woodworth, Kidd, Sung, Miyakawa, Bear, Weinberg, Sheng: "Smaller dendritic spines, weaker synaptic transmission, but enhanced spatial learning in mice lacking Shank1." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 7, pp. 1697-708, 2008

Images

Product Details anti-SHANK1 Antibody Target Details SHANK1 Application Details Handling References for anti-SHANK1 antibody (ABIN863122) back to top
Supplier Images
Western Blotting (WB) image for anti-SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1) (AA 469-691) antibody (ABIN863122) Shank1 Brain Membranes Western Blotting.