anti-Mouse (Murine) CD137 antibody for Western Blotting

Recommended CD137 Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) Antibodies
  • TNFRSF9
  • zgc:136557
  • 4-1BB
  • CD137
  • CDw137
  • ILA
  • A930040I11Rik
  • AA408498
  • AI325004
  • Cd137
  • Ly63
  • tumor necrosis factor receptor superfamily, member 9
  • tumor necrosis factor receptor superfamily, member 9a
  • TNFRSF9
  • tnfrsf9a
  • LOC100348914
  • Tnfrsf9
Epitope
AA 24-187
22
15
6
5
5
4
3
3
3
2
2
1
1
1
Reactivity
Mouse (Murine)
217
127
21
15
14
10
3
2
2
2
2
2
Host
Goat
119
105
56
26
19
Clonality
Polyclonal
Conjugate
This CD137 antibody is un-conjugated
29
23
15
11
11
10
9
6
6
6
6
6
6
5
4
4
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
Application
Agonist (Agon), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
210
137
97
21
18
17
13
11
8
7
5
5
3
2
2
2
2
1
1
1
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Available images

Catalog No. ABIN5012832
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN740445 IF (p) IHC (p) WB Rabbit IgG AA 115-165 Log in to see Polyclonal 2
1 ABIN2656913 FACS IHC WB APC Hamster IgG Log in to see 17B5 5
1 ABIN4899797 CyTOF ELISA (Capture) FACS WB Rat IgG2a AA 24-187 Log in to see 158332
1 ABIN360874 FACS WB Rat IgG2 Extracellular Domain Log in to see 4G33
1 ABIN740454 IHC (p) WB HRP Rabbit IgG AA 115-165 Log in to see Polyclonal
1 ABIN740447 IHC (p) WB Biotin Rabbit IgG AA 115-165 Log in to see Polyclonal
1 ABIN2656914 FACS IHC WB APC Hamster IgG Log in to see 17B5 5
1 ABIN1169064 FACS WB Rat Log in to see Polyclonal
1 ABIN2755639 IC IF WB Rabbit Center Log in to see Polyclonal
1 ABIN1169067 FACS WB Rat IgG1 Log in to see M4173
1 ABIN4251906 BP Neut WB Rat IgG2 Log in to see RM0344-6J43
1 ABIN4251907 CyTOF FACS WB Rat IgG2 Log in to see RM0068-4G33
1 ABIN4899796 ELISA (Detection) FACS WB Biotin Goat IgG AA 24-187 Log in to see Polyclonal
1 ABIN2962158 FACS WB Rat IgG1 kappa Log in to see M4173
1 ABIN4251909 FACS WB DyLight 350 Rat IgG2 Log in to see RM0068-4G33
1 ABIN4251911 FACS WB DyLight 405 Rat IgG2 Log in to see RM0068-4G33
1 ABIN4251916 FACS WB PerCP Rat IgG2 Log in to see RM0068-4G33
1 ABIN4251920 FACS WB DyLight 755 Rat IgG2 Log in to see RM0068-4G33
1 ABIN4251923 FACS WB DyLight 488 Rat IgG2 Log in to see RM0068-4G33
1 ABIN4251926 FACS WB DyLight 680 Rat IgG2 Log in to see RM0068-4G33

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) Antibodies
Epitope AA 24-187
(22), (15), (6), (5), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Reactivity Mouse (Murine)
(217), (127), (21), (15), (14), (10), (3), (2), (2), (2), (2), (2)
Host Goat
(119), (105), (56), (26), (19)
Clonality
Polyclonal
Conjugate This CD137 antibody is un-conjugated
(29), (23), (15), (11), (11), (10), (9), (6), (6), (6), (6), (6), (6), (5), (4), (4), (4), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Agonist (Agon), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(210), (137), (97), (21), (18), (17), (13), (11), (8), (7), (5), (5), (3), (2), (2), (2), (2), (1), (1), (1)
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Product Details anti-CD137 Antibody

Target Details CD137 Application Details Handling Images
Specificity Detects mouse 4-1BB/TNFRSF9/CD137 in direct ELISAs and Western blots. In direct ELISAs, approximately 100 % cross-reactivity with recombinant rat 4-1BB/TNFRSF9 is observed. In Western blots, less than 5 % cross-reactivity with recombinant human 4-1BB and recombinant mouse EDAR is observed.
Purification Antigen Affinity-purified
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse 4-1BB/TNFRSF9/CD137, Val24-Leu187, Accession # P20334
Isotype IgG

Target Details CD137

Product Details anti-CD137 Antibody Application Details Handling Images back to top
Antigen
Alternative Name 4-1BB/TNFRSF9/CD137 (TNFRSF9 Antibody Abstract)
Background Alternative Names: 41BB, 4-1BB, 4-1BB ligand receptor, CD137, CD137 antigen, CD137MGC2172, CDw137, FLJ43501, ILA, ILAhomolog of mouse 4-1BB, induced by lymphocyte activation (ILA), interleukin-activated receptor, homolog of mouse Ly63, receptor protein 4-1BB, T cell antigen ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, tumor necrosis factor receptor superfamily member 9, tumor necrosis factor receptor superfamily, member 9
Gene ID 3604
UniProt P20334

Application Details

Product Details anti-CD137 Antibody Target Details CD137 Handling Images back to top
Application Notes Western Blot 0.5 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunohistochemistry 1-15 μg/mL
Agonist Activity
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CD137 Antibody Target Details CD137 Application Details Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

Images

Product Details anti-CD137 Antibody Target Details CD137 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-187) antibody (ABIN5012832) 4-1BB/TNFRSF9/CD137 in Mouse Spleen. 4-1BB/TNFRSF9/CD137 was detected in perfusion fi...
Western Blotting (WB) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-187) antibody (ABIN5012832) Detection of Mouse 4-1BB/TNFRSF9/ CD137 by Western Blot. Western blot shows lysates o...
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-187) antibody (ABIN5012832) Detection of 4-1BB/TNFRSF9/CD137 in Activated Mouse Splenocytes by Flow Cytometry. Ac...
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-187) antibody (ABIN5012832) Detection of 4-1BB/TNFRSF9/CD137 in Resting Mouse Splenocytes by Flow Cytometry. Rest...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-187) antibody (ABIN5012832) 4-1BB/TNFRSF9/CD137 in Mouse Spleen. 4-1BB/TNFRSF9/CD137 was detected in perfusion fi...
Western Blotting (WB) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-187) antibody (ABIN5012832) Detection of Mouse 4-1BB/TNFRSF9/ CD137 by Western Blot. Western blot shows lysates o...
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-187) antibody (ABIN5012832) Detection of 4-1BB/TNFRSF9/CD137 in Activated Mouse Splenocytes by Flow Cytometry. Ac...
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-187) antibody (ABIN5012832) Detection of 4-1BB/TNFRSF9/CD137 in Resting Mouse Splenocytes by Flow Cytometry. Rest...