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anti-Chicken CD28 antibody for Flow Cytometry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Chicken, Turkey
314
307
102
37
26
12
6
5
4
2
2
2
1
1
1
1
Host
Mouse
359
189
77
42
25
20
7
Clonality
Monoclonal
Conjugate
This CD28 antibody is conjugated to FITC
106
89
66
41
10
8
7
7
7
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS)
540
174
114
95
92
43
41
34
27
22
13
8
4
3
3
3
3
3
2
2
1
1
1
1
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Catalog No. ABIN371358
$ 489.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010476487 ABIN288755 FACS FITC Mouse IgG1 Log in to see AV7
0.0010476487 ABIN288756 FACS PE Mouse IgG1 Log in to see AV7
0.0010476487 ABIN288754 FACS IP Biotin Mouse IgG1 Log in to see AV7
0.0010476487 ABIN371357 FACS Biotin Mouse IgG1 Log in to see AV7 2
0.0010476487 ABIN371360 FACS PE Mouse IgG1 Log in to see AV7 2
0.0010476487 ABIN371359 Func FACS IP Mouse IgG1 Log in to see AV7 2
0.0010476487 ABIN593898 FACS PE Mouse IgG2b Log in to see
0.0010476487 ABIN135535 FACS PE Mouse IgG1 Log in to see AV7 2
0.0010476487 ABIN135533 FACS FITC Mouse IgG1 Log in to see AV7 2
0.0010476487 ABIN135534 FACS IP Biotin Mouse IgG1 Log in to see AV7 2
0.0010476487 ABIN284174 FACS IP Mouse IgG1 Log in to see AV7
0.0010476487 ABIN1826941 FACS IP Biotin Mouse IgG1 Log in to see AV7
0.0010476487 ABIN1826942 FACS FITC Mouse IgG1 Log in to see AV7
0.0010476487 ABIN1826943 FACS PE Mouse IgG1 Log in to see AV7
0.0010476487 ABIN135532 FACS IP Mouse IgG1 Log in to see AV7 3
0.0010476487 ABIN1820216 FACS IP Mouse IgG1 Log in to see AV7
0.0010476487 ABIN2593002 FACS PE Mouse IgG1 Log in to see AV7
0.0010476487 ABIN212796 FACS IHC (fro) IP Mouse IgG2a Log in to see
0.0010476487 ABIN2593001 FACS Biotin Mouse IgG1 Log in to see AV7
0.0010476487 ABIN2593003 FACS FITC Mouse IgG1 Log in to see AV7

Top referenced anti-CD28 antibody for Flow Cytometry

Similar anti-CD28 Antibodies

Application / Reactivity Chicken Turkey
Western Blotting (WB) 4 Antibodies
Immunoprecipitation (IP) 23 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Functional Studies (Func) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 38 Antibodies 5 Antibodies

General

Antigen CD28 Antibodies
Reactivity Chicken, Turkey
(314), (307), (102), (37), (26), (12), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(359), (189), (77), (42), (25), (20), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is conjugated to FITC
(106), (89), (66), (41), (10), (8), (7), (7), (7), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS)
(540), (174), (114), (95), (92), (43), (41), (34), (27), (22), (13), (8), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN371358) Images
Specificity This antibody recognizes Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50 % amino acid sequence identity with mammalian CD28.
Characteristics Synonyms: TP44, T-cell-specific surface glycoprotein CD28
Clone AV7
Isotype IgG1

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN371358) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral gamma/delta+ T cells are CD28-negative. (Ref.1) Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both Vbeta1- and Vbeta2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3. (Ref.2)Synonyms: T-cell-specific surface glycoprotein CD28, TP44
Gene ID 396249
UniProt P31043
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN371358) Images back to top
Application Notes Flow Cytometry: < / = 1 μg/10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN371358) Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CD28 Antibody (ABIN371358)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Young, Davison, Tregaskes, Rennie, Vainio: "Monomeric homologue of mammalian CD28 is expressed on chicken T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 8, pp. 3848-51, 1994

Arstila, Vainio, Lassila: "Evolutionarily conserved function of CD28 in alpha beta T cell activation." in: Scandinavian journal of immunology, Vol. 40, Issue 3, pp. 368-71, 1994

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN371358) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28)  (FITC) (ABIN371358) anti-CD28 (CD28) antibody (FITC)