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anti-Dog (Canine) CD28 antibody for Flow Cytometry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Dog (Canine)
445
374
106
72
44
34
26
26
23
23
12
6
1
1
1
1
1
Host
Mouse
515
203
77
68
28
25
12
Clonality
Monoclonal
Conjugate
This CD28 antibody is conjugated to APC
118
99
78
40
17
17
15
13
13
12
11
10
9
9
9
9
9
9
9
8
8
8
6
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS)
748
275
183
132
123
118
117
114
89
66
22
13
8
4
3
3
3
3
3
2
2
1
1
1
1
1
1
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Available images

Catalog No. ABIN2678353
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Similar anti-CD28 Antibodies

Application / Reactivity Dog (Canine)
Flow Cytometry (FACS) 1 Antibodies

General

Antigen CD28 Antibodies
Reactivity Dog (Canine)
(445), (374), (106), (72), (44), (34), (26), (26), (23), (23), (12), (6), (1), (1), (1), (1), (1)
Host Mouse
(515), (203), (77), (68), (28), (25), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is conjugated to APC
(118), (99), (78), (40), (17), (17), (15), (13), (13), (12), (11), (10), (9), (9), (9), (9), (9), (9), (9), (8), (8), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS)
(748), (275), (183), (132), (123), (118), (117), (114), (89), (66), (22), (13), (8), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity The 5B8 monoclonal antibody reacts with canine CD28, which is expressed by thymocytes, a majority of T cells, and a subset of NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

The 5B8 antibody has been reported to have agonist activity. The 5B8 and 1C6 antibodies recognize different epitopes of canine CD28.
Characteristics The 5B8 monoclonal antibody reacts with canine CD28, which is expressed by thymocytes, a majority of T cells, and a subset of NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. The 5B8 antibody has been reported to have agonist activity.
Clone 5B8
Isotype IgG1 kappa

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes Applications reported: This 5B8 antibody has been reported for use in flow cytometric analysis.

Applications tested: This 5B8 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28)  (APC) (ABIN2678353) anti-CD28 (CD28) antibody (APC)