We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Human|
|Activation (Act)||1 Antibodies|
|Biochemical Assay (BCA)||3 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||188 Antibodies|
|Functional Studies (Func)||44 Antibodies|
|Immunocytochemistry (ICC)||22 Antibodies|
|Immunofluorescence (IF)||30 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||29 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||4 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||35 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies|
|Immunoprecipitation (IP)||37 Antibodies|
|Intracellular Flow Cytometry (ICFC)||1 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Western Blotting (WB)||95 Antibodies|
|Conjugate||This CD28 antibody is conjugated to PerCP-Cy5.5 Alternatives|
Flow Cytometry (FACS), Mass Cytometry (CyTOF)
|9 references available|
|Supplier||Log in to see|
Product Details anti-CD28 AntibodyTarget Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN2660336) Images
|Cross-Reactivity||Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Pigtailed Macaque, Capuchin Monkey, Sooty mangabey (Cercocebus atys), Squirrel Monkey (Saimiri spec.)|
|Purification||The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.|
Target Details CD28Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN2660336) Images back to top
|Alternative Name||CD28 (CD28 Antibody Abstract)|
|Background||CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway|
Application DetailsProduct Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN2660336) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN2660336) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Protect from prolonged exposure to light. Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-CD28 Antibody (ABIN2660336)Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Rivollier, Mazzorana, Tebib et al.: "Immature dendritic cell transdifferentiation into osteoclasts: a novel pathway sustained by the rheumatoid arthritis microenvironment." in: Blood, Vol. 104, Issue 13, pp. 4029-37, 2004 (PubMed).
Jeong, Qiao, Nascimbeni et al.: "Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates." in: Journal of virology, Vol. 78, Issue 13, pp. 6995-7003, 2004 (PubMed).
Scharschmidt, Wegener, Heissmeyer et al.: "Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling." in: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3860-73, 2004 (PubMed).
Marti, Krause, Post et al.: "Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 197-206, 2001 (PubMed).
Tazi, Moreau, Bergeron et al.: "Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3511-5, 1999 (PubMed).
Galea-Lauri, Darling, Gan et al.: "Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 62-70, 1999 (PubMed).
Nunes, Klasen, Franco et al.: "Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity." in: The Biochemical journal, Vol. 293 ( Pt 3), pp. 835-42, 1993 (PubMed).
Linsley, Ledbetter: "The role of the CD28 receptor during T cell responses to antigen." in: Annual review of immunology, Vol. 11, pp. 191-212, 1993 (PubMed).
June, Bluestone, Nadler et al.: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, 1994 (PubMed).
ImagesProduct Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN2660336) back to top