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anti-Human CD28 antibody for ELISA

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Epitope
pTyr218
21
16
15
14
11
11
9
8
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
441
362
106
69
44
31
26
23
23
23
12
6
2
1
1
1
1
Host
Rabbit
512
203
76
66
25
23
7
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
115
96
75
41
17
17
15
13
12
12
11
10
9
9
9
9
9
9
9
8
8
8
6
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Western Blotting (WB)
734
265
178
131
122
117
116
111
86
66
22
13
4
3
3
3
3
3
2
2
1
1
1
1
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Catalog No. ABIN1531543
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012100277 ABIN2464509 ELISA WB Goat Internal Region Log in to see Polyclonal
0.0012100277 ABIN2659213 ELISA PE-Cy7 Mouse IgG1 kappa Log in to see CD28-2 9
0.0012100277 ABIN2662242 ELISA Pacific Blue Mouse IgG1 kappa Log in to see CD28-2 9
0.0012100277 ABIN793286 ELISA WB Goat Internal Region Log in to see Polyclonal
0.0012100277 ABIN610015 Func FACS ELISA PE Mouse IgG1, kappa Log in to see ANC28-1-5D10
0.0012100277 ABIN610014 Func FACS ELISA FITC Mouse IgG1, kappa Log in to see ANC28-1-5D10
0.0012100277 ABIN610013 Func FACS ELISA Biotin Mouse IgG1, kappa Log in to see ANC28-1-5D10
0.0012100277 ABIN1584203 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012100277 ABIN1998809 ELISA Rabbit IgG AA 1-152 Log in to see Polyclonal 1
0.0012100277 ABIN1975063 ICC ELISA WB PE Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0012100277 ABIN205114 ELISA WB Rabbit IgG AA 72-85 Log in to see Polyclonal
0.0012100277 ABIN1970482 ICC ELISA WB FITC Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0012100277 ABIN1961665 ICC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0012100277 ABIN1964045 ICC ELISA WB APC Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0012100277 ABIN2659214 ELISA PE-Cy7 Mouse IgG1 kappa Log in to see CD28-2 11
0.0012100277 ABIN2662243 ELISA Pacific Blue Mouse IgG1 kappa Log in to see CD28-2 9
0.0012100277 ABIN1966662 ICC ELISA WB Biotin Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0012100277 ABIN1998807 ELISA Rabbit IgG AA 1-152 Log in to see Polyclonal 1
0.0012100277 ABIN1452525 ELISA WB Rabbit IgG pTyr218, C-Term Log in to see Polyclonal
0.0012100277 ABIN203926 FACS ELISA Biotin Mouse IgG1, kappa Log in to see

Similar anti-CD28 Antibodies

Application / Reactivity Mouse (Murine)
Activation (Act) 2 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA 22 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 287 Antibodies
Functional Studies (Func) 27 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 91 Antibodies
In vitro Assay (in vitro) 7 Antibodies
Intracellular Flow Cytometry (ICFC) 9 Antibodies

General

Antigen CD28 Antibodies
Epitope pTyr218
(21), (16), (15), (14), (11), (11), (9), (8), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(441), (362), (106), (69), (44), (31), (26), (23), (23), (23), (12), (6), (2), (1), (1), (1), (1)
Host Rabbit
(512), (203), (76), (66), (25), (23), (7)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(115), (96), (75), (41), (17), (17), (15), (13), (12), (12), (11), (10), (9), (9), (9), (9), (9), (9), (9), (8), (8), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(734), (265), (178), (131), (122), (117), (116), (111), (86), (66), (22), (13), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity CD28 (Phospho-Tyr218) Antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218.
Isotype IgG

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background Molecular weight of target protein: 25 kDa
Synonyms: SCFR, Proto-oncogene tyrosine-protein kinase Kit, c-kit, CD117 antigen
Molecular Weight 25 kDa
Gene ID 940
OMIM 186760
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes WB: 1:500-1:1000
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.591629 (NCBI Gene Symbol: CD28)

Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 antibody (CD28) (pTyr218) (ABIN1531543) Western blot analysis of extracts from HepG2 cells treated with nocodazole 1ug/ml 16h...