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anti-Human CD28 antibody for ELISA

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
Internal Region
16
16
14
12
11
11
11
10
8
7
6
4
4
4
3
3
3
3
1
1
1
1
1
1
Reactivity
Human
467
388
102
70
51
34
27
26
25
22
15
6
3
3
3
2
1
1
1
Host
Goat
504
193
90
67
40
26
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
110
92
72
36
20
18
18
18
17
16
16
12
12
12
12
11
10
10
8
7
6
6
6
6
6
5
4
3
2
2
2
2
2
2
2
2
2
Application
ELISA, Western Blotting (WB)
750
208
184
146
140
136
129
127
88
72
26
13
13
9
8
6
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN2464509
$ 481.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1531543 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN2659213 ELISA PE-Cy7 Mouse IgG1 kappa Log in to see CD28-2 9
1 ABIN2662242 ELISA Pacific Blue Mouse IgG1 kappa Log in to see CD28-2 9
1 ABIN793286 ELISA WB Goat AA 73-87 Log in to see Polyclonal
1 ABIN3187775 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1998809 ELISA Rabbit IgG AA 1-152 Log in to see Polyclonal 1
1 ABIN1998807 ELISA Rabbit IgG AA 1-152 Log in to see Polyclonal 1
1 ABIN1584203 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2659214 ELISA PE-Cy7 Mouse IgG1 kappa Log in to see CD28-2 11
1 ABIN2662243 ELISA Pacific Blue Mouse IgG1 kappa Log in to see CD28-2 9
1 ABIN610013 Func FACS ELISA Biotin Mouse IgG1, kappa Log in to see ANC28-1-5D10
1 ABIN610014 Func FACS ELISA FITC Mouse IgG1, kappa Log in to see ANC28-1-5D10
1 ABIN610015 Func FACS ELISA PE Mouse IgG1, kappa Log in to see ANC28-1-5D10
1 ABIN3182245 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN205114 ELISA WB Rabbit IgG AA 72-85 Log in to see Polyclonal
1 ABIN610012 Func FACS ELISA Mouse IgG1, kappa Log in to see ANC28-1-5D10
1 ABIN3028547 IF ELISA WB Rabbit Ig Fraction AA 182-208 Log in to see Polyclonal
1 ABIN1998805 CC ELISA Mouse IgG1 AA 1-152 Log in to see 9H6E2
1 ABIN1452525 ELISA WB Rabbit IgG pTyr218, C-Term Log in to see Polyclonal
1 ABIN203926 FACS ELISA Biotin Mouse IgG1, kappa Log in to see

Similar anti-CD28 Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Agonist (Agon) 3 Antibodies
Biochemical Assay (BCA) 3 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies
ELISA 122 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 343 Antibodies
Functional Studies (Func) 48 Antibodies
Immunocytochemistry (ICC) 108 Antibodies
Immunofluorescence (IF) 128 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 62 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 127 Antibodies

General

Antigen CD28 Antibodies
Epitope Internal Region
(16), (16), (14), (12), (11), (11), (11), (10), (8), (7), (6), (4), (4), (4), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Reactivity Human
(467), (388), (102), (70), (51), (34), (27), (26), (25), (22), (15), (6), (3), (3), (3), (2), (1), (1), (1)
Host Goat
(504), (193), (90), (67), (40), (26), (12), (1)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(110), (92), (72), (36), (20), (18), (18), (18), (17), (16), (16), (12), (12), (12), (12), (11), (10), (10), (8), (7), (6), (6), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Western Blotting (WB)
(750), (208), (184), (146), (140), (136), (129), (127), (88), (72), (26), (13), (13), (9), (8), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN2457078
Specificity The immunizing peptide corresponds to a part of the extra-cellular domain.
Purification CD28 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen CD28 antibody was raised against a 15 amino acid synthetic peptide near the internal region of CD28.

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Gene ID 940
NCBI Accession NP_006130
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 48 kDa band observed in human lymph node and tonsil lysates (calculated MW of 25.1 kDa according to NP_006130.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Concentration 500 μg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 antibody (CD28) (Internal Region) (ABIN2464509) anti-CD28 (CD28) (Internal Region) antibody