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anti-Human CD28 antibody for ELISA

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
Internal Region
21
16
15
15
11
11
11
10
6
4
4
4
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human
466
407
106
72
44
34
26
26
24
24
12
6
1
1
1
1
Host
Goat
523
214
84
72
40
27
12
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
120
99
78
38
21
20
17
16
16
15
14
12
12
12
12
11
10
10
10
7
6
6
5
4
4
4
4
3
2
2
2
2
2
2
2
2
2
Application
ELISA, Western Blotting (WB)
784
213
181
142
140
135
133
126
97
67
26
13
13
8
6
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN2464509
$ 481.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1531543 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN2659213 ELISA PE-Cy7 Mouse IgG1 kappa Log in to see CD28-2 9
1 ABIN3187775 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN2662242 ELISA Pacific Blue Mouse IgG1 kappa Log in to see CD28-2 9
1 ABIN793286 ELISA WB Goat Internal Region Log in to see Polyclonal
1 ABIN1998809 ELISA Rabbit IgG AA 1-152 Log in to see Polyclonal 1
1 ABIN610015 Func FACS ELISA PE Mouse IgG1, kappa Log in to see ANC28-1-5D10
1 ABIN610013 Func FACS ELISA Biotin Mouse IgG1, kappa Log in to see ANC28-1-5D10
1 ABIN610014 Func FACS ELISA FITC Mouse IgG1, kappa Log in to see ANC28-1-5D10
1 ABIN1584203 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1966662 ICC ELISA WB Biotin Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
1 ABIN1975063 ICC ELISA WB PE Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
1 ABIN2659214 ELISA PE-Cy7 Mouse IgG1 kappa Log in to see CD28-2 11
1 ABIN3182245 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN1998807 ELISA Rabbit IgG AA 1-152 Log in to see Polyclonal 1
1 ABIN2662243 ELISA Pacific Blue Mouse IgG1 kappa Log in to see CD28-2 9
1 ABIN1961665 ICC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
1 ABIN1964045 ICC ELISA WB APC Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
1 ABIN1970482 ICC ELISA WB FITC Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
1 ABIN1452525 ELISA WB Rabbit IgG pTyr218, C-Term Log in to see Polyclonal

Similar anti-CD28 Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Agonist (Agon) 3 Antibodies
Biochemical Assay (BCA) 3 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies
ELISA 126 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 337 Antibodies
Functional Studies (Func) 52 Antibodies
Immunocytochemistry (ICC) 113 Antibodies
Immunofluorescence (IF) 123 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 62 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 123 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 44 Antibodies
Immunoprecipitation (IP) 54 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies

General

Antigen CD28 Antibodies
Epitope Internal Region
(21), (16), (15), (15), (11), (11), (11), (10), (6), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(466), (407), (106), (72), (44), (34), (26), (26), (24), (24), (12), (6), (1), (1), (1), (1)
Host Goat
(523), (214), (84), (72), (40), (27), (12)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(120), (99), (78), (38), (21), (20), (17), (16), (16), (15), (14), (12), (12), (12), (12), (11), (10), (10), (10), (7), (6), (6), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Western Blotting (WB)
(784), (213), (181), (142), (140), (135), (133), (126), (97), (67), (26), (13), (13), (8), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN2457078
Specificity The immunizing peptide corresponds to a part of the extra-cellular domain.
Purification CD28 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen CD28 antibody was raised against a 15 amino acid synthetic peptide near the internal region of CD28.

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Gene ID 940
NCBI Accession NP_006130
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 48 kDa band observed in human lymph node and tonsil lysates (calculated MW of 25.1 kDa according to NP_006130.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Concentration 500 μg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 antibody (CD28) (Internal Region) (ABIN2464509) anti-CD28 (CD28) (Internal Region) antibody