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anti-Human CD28 antibody for ELISA

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Epitope
Internal Region
21
15
15
14
11
11
10
8
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
444
374
106
72
44
34
26
26
23
23
12
6
2
1
1
1
1
Host
Goat
516
203
77
68
28
24
12
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
118
99
78
41
17
17
15
13
13
12
11
10
9
9
9
9
9
9
9
8
8
8
6
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Western Blotting (WB)
749
275
183
131
123
117
117
114
89
66
22
13
8
4
3
3
3
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN2464509
$ 481.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012045924 ABIN1531543 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
0.0012045924 ABIN2659213 ELISA PE-Cy7 Mouse IgG1 kappa Log in to see CD28-2 9
0.0012045924 ABIN2662242 ELISA Pacific Blue Mouse IgG1 kappa Log in to see CD28-2 9
0.0012045924 ABIN793286 ELISA WB Goat Internal Region Log in to see Polyclonal
0.0012045924 ABIN610013 Func FACS ELISA Biotin Mouse IgG1, kappa Log in to see ANC28-1-5D10
0.0012045924 ABIN610014 Func FACS ELISA FITC Mouse IgG1, kappa Log in to see ANC28-1-5D10
0.0012045924 ABIN610015 Func FACS ELISA PE Mouse IgG1, kappa Log in to see ANC28-1-5D10
0.0012045924 ABIN1584203 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012045924 ABIN1998809 ELISA Rabbit IgG AA 1-152 Log in to see Polyclonal 1
0.0012045924 ABIN1961665 ICC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0012045924 ABIN1966662 ICC ELISA WB Biotin Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0012045924 ABIN1970482 ICC ELISA WB FITC Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0012045924 ABIN2659214 ELISA PE-Cy7 Mouse IgG1 kappa Log in to see CD28-2 11
0.0012045924 ABIN2662243 ELISA Pacific Blue Mouse IgG1 kappa Log in to see CD28-2 9
0.0012045924 ABIN1964045 ICC ELISA WB APC Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0012045924 ABIN1975063 ICC ELISA WB PE Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0012045924 ABIN1998807 ELISA Rabbit IgG AA 1-152 Log in to see Polyclonal 1
0.0012045924 ABIN203926 FACS ELISA Biotin Mouse IgG1, kappa Log in to see
0.0012045924 ABIN1452525 ELISA WB Rabbit IgG pTyr218, C-Term Log in to see Polyclonal
0.0012045924 ABIN1972544 ICC ELISA WB HRP Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal

Similar anti-CD28 Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Biochemical Assay (BCA) 3 Antibodies
ELISA 102 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 322 Antibodies
Functional Studies (Func) 68 Antibodies
Immunocytochemistry (ICC) 106 Antibodies
Immunofluorescence (IF) 118 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 47 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 139 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antibodies
Immunoprecipitation (IP) 115 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Western Blotting (WB) 113 Antibodies

General

Antigen CD28 Antibodies
Epitope Internal Region
(21), (15), (15), (14), (11), (11), (10), (8), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(444), (374), (106), (72), (44), (34), (26), (26), (23), (23), (12), (6), (2), (1), (1), (1), (1)
Host Goat
(516), (203), (77), (68), (28), (24), (12)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(118), (99), (78), (41), (17), (17), (15), (13), (13), (12), (11), (10), (9), (9), (9), (9), (9), (9), (9), (8), (8), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(749), (275), (183), (131), (123), (117), (117), (114), (89), (66), (22), (13), (8), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN2457078
Specificity The immunizing peptide corresponds to a part of the extra-cellular domain.
Purification CD28 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen CD28 antibody was raised against a 15 amino acid synthetic peptide near the internal region of CD28.

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Gene ID 940
NCBI Accession NP_006130
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 48 kDa band observed in human lymph node and tonsil lysates (calculated MW of 25.1 kDa according to NP_006130.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Concentration 500 μg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 antibody (CD28) (Internal Region) (ABIN2464509) anti-CD28 (CD28) (Internal Region) antibody