This CD28 antibody is un-conjugated
ELISA, Western Blotting (WB)
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Blocking peptide for this product available: ABIN2457078
The immunizing peptide corresponds to a part of the extra-cellular domain.
CD28 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CD28 antibody was raised against a 15 amino acid synthetic peptide near the internal region of CD28.
CD28 Antibody Abstract)
Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 48 kDa band observed in human lymph node and tonsil lysates (calculated MW of 25.1 kDa according to NP_006130.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL.
For Research Use only
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Minimize freezing and thawing.
Aliquot and store at -20 °C.
anti-CD28 (CD28) (Internal Region) antibody