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anti-Human CD28 antibody for Immunocytochemistry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Reactivity
Human
467
388
102
70
51
34
27
26
25
22
15
6
3
3
3
2
1
1
1
Host
Goat
504
193
90
67
40
26
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
110
92
72
36
20
18
18
18
17
16
16
12
12
12
12
11
10
10
8
7
6
6
6
6
6
5
4
3
2
2
2
2
2
2
2
2
2
Application
Agonist (Agon), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
749
208
184
146
141
136
128
127
88
72
26
13
13
9
8
6
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN5012855
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN638435 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 7
1 ABIN775705 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 5
1 ABIN349710 FACS Func ICC IHC (f) IP WB PE Mouse IgG1 Log in to see CD28-2 6
1 ABIN349705 FACS Func ICC IHC (f) IP WB APC Mouse IgG1 Log in to see CD28-2 6
1 ABIN349707 FACS Func ICC IHC (f) IP WB FITC Mouse IgG1 Log in to see CD28-2 4
1 ABIN457388 FACS Func ICC IHC (f) IP WB PerCP Mouse IgG1 Log in to see CD28-2 6
1 ABIN2618450 FACS Func ICC IHC (fro) IHC (p) IP Syrian Hamster IgG Log in to see 37-51
1 ABIN4291200 CyTOF FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN1959966 Func ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN1964045 ELISA ICC IF WB APC Rabbit IgG AA 182-208 Log in to see Polyclonal
1 ABIN1961665 ELISA ICC IF WB Alkaline Phosphatase (AP) Rabbit IgG AA 182-208 Log in to see Polyclonal
1 ABIN1966662 ELISA ICC IF WB Biotin Rabbit IgG AA 182-208 Log in to see Polyclonal
1 ABIN1975063 ELISA ICC IF WB PE Rabbit IgG AA 182-208 Log in to see Polyclonal
1 ABIN1970482 ELISA ICC IF WB FITC Rabbit IgG AA 182-208 Log in to see Polyclonal
1 ABIN1972544 ELISA ICC IF WB HRP Rabbit IgG AA 182-208 Log in to see Polyclonal
1 ABIN3200893 FACS Func ICC IHC (fro) IP WB PE Mouse IgG1 Log in to see CD28-2
1 ABIN3199704 FACS Func ICC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN3200894 FACS Func ICC IHC (fro) IP WB PerCP Mouse IgG1 Log in to see CD28-2
1 ABIN3200892 FACS Func ICC IHC (fro) IP WB FITC Mouse IgG1 Log in to see CD28-2
1 ABIN4291270 ELISA FACS ICC IF IHC (fro) IHC (p) DyLight 488 Mouse IgG1 kappa Log in to see C28-75

Top referenced anti-CD28 antibody for Immunocytochemistry

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Application / Reactivity Human
Activation (Act) 1 Antibodies
Agonist (Agon) 3 Antibodies
Biochemical Assay (BCA) 3 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies
ELISA 122 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 343 Antibodies
Functional Studies (Func) 48 Antibodies
Immunocytochemistry (ICC) 108 Antibodies
Immunofluorescence (IF) 128 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 62 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 127 Antibodies

General

Antigen CD28 Antibodies
Reactivity Human
(467), (388), (102), (70), (51), (34), (27), (26), (25), (22), (15), (6), (3), (3), (3), (2), (1), (1), (1)
Host Goat
(504), (193), (90), (67), (40), (26), (12), (1)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(110), (92), (72), (36), (20), (18), (18), (18), (17), (16), (16), (12), (12), (12), (12), (11), (10), (10), (8), (7), (6), (6), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Agonist (Agon), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(749), (208), (184), (146), (141), (136), (128), (127), (88), (72), (26), (13), (13), (9), (8), (6), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity Detects human CD28 in direct ELISAs and Western blots. In direct ELISAs, approximately 20 % cross-reactivity with recombinant mouse CD28 is observed.
Purification Immunogen affinity purified
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD28
Isotype IgG

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background Alternative Names: CD28, CD28 antigen, CD28 antigen (Tp44), CD28 Molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44
Gene ID 940
Research Area Surface Receptors of Immune Cells, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Apoptosis/Necrosis, Immunology
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Agonist Activity 0.3-0.6 μg/mL
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-CD28 antibody (CD28) (ABIN5012855) CD28 in Human PBMCs. CD28 was detected in immersion fixed human peripheral blood mono...
Functional Studies (Func) image for anti-CD28 antibody (CD28) (ABIN5012855) Human CD28 Antibody Enhances IL-2 Secretion in Jurkat Cells. Human CD28 Antigen Affin...