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|Application / Reactivity||Human|
|Activation (Act)||1 Antibodies|
|Biochemical Assay (BCA)||3 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||319 Antibodies|
|Functional Studies (Func)||68 Antibodies|
|Immunocytochemistry (ICC)||106 Antibodies|
|Immunofluorescence (IF)||114 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||24 Antibodies|
|Immunohistochemistry (IHC)||45 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||6 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||133 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||48 Antibodies|
|Immunoprecipitation (IP)||114 Antibodies|
|Intracellular Flow Cytometry (ICFC)||1 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Conjugate||This CD28 antibody is un-conjugated Alternatives|
Immunohistochemistry (Acetone-fixed) (IHC (af)), Flow Cytometry (FACS)
|Supplier||Log in to see|
Product Details anti-CD28 AntibodyTarget Details CD28 Application Details Handling Images
Rat anti Human CD28 antibody, clone YTH913.12 recognizes human CD28, a ~44 kDa single pass type 1 trans-membrane protein expressed as a homodimer on a major subset of human T-cells (Thompson et al. 1989), responsible for activation of these cells via interaction with theTCR. CD28 is involved in the tuning of the T-cell for activation via TCR, lowering the threshold for activation from around 8000 triggered TCRs to approximately 1500 (Viola et al.1996).
CD28 along with CD152, also known as CTLA-4 acts as a co-receptor for the co-stimulatory molecules CD80 and CD86 (Azuma et al. 1993). CD28 offers a positive stimulatory role on ligation of CD80 and CD86 while CTLA-4 offers a negative feedback signal preventing CD28 mediated T-cell activation of CD86 (Krummel et al. 1995).
Rat anti human CD28, clone YTH913.12 has been reported to recognize an epitope of CD28 expressed by NK cells, which is not recognized by other anti human CD28 clones such as 9.3 and CD28.2 (Galea-Lauri et al 1999.) Other reports however have failed to demonstrate CD28 staining on peripheral blood derived KK cells using clone YTH913.12 (Wilson et al. 1999).
Rat Anti Human Cd28:Low Endotoxin
Protein Size: 220
|Endotoxin Level||Low Endotoxin|
|Immunogen||The immunogen for anti-CD28 antibody: human peripheral blood T-cells|
Target Details CD28Product Details anti-CD28 Antibody Application Details Handling Images back to top
|Alternative Name||CD28 (CD28 Antibody Abstract)|
|Background||Rat Anti Human Cd28: Low Endotoxin|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway|
Application DetailsProduct Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
|Application Notes||Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 106cells in 100 μL.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
|Handling Advice||Avoid repeat freeze-thaw cycles.|
Store at -20 °C only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
ImagesProduct Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top