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anti-Human CD28 antibody for Immunohistochemistry (Acetone-fixed)

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Human
444
374
106
72
44
34
26
26
23
23
12
6
2
1
1
1
1
Host
Rat
516
203
77
67
28
25
12
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
118
99
78
41
17
17
15
13
13
12
11
10
9
9
9
9
9
9
9
8
8
8
6
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunohistochemistry (Acetone-fixed) (IHC (af)), Flow Cytometry (FACS)
748
275
183
132
123
118
117
114
89
66
22
13
8
3
3
3
3
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN2164999
$ 814.29
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN2167470 IHC (af) FACS Rat IgG2b Log in to see
0.0008709465 ABIN2167469 IHC (af) FACS Rat IgG2b Log in to see
0.0008709465 ABIN2165702 IHC (af) FACS Rat IgG2b Log in to see

Similar anti-CD28 Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Biochemical Assay (BCA) 3 Antibodies
ELISA 102 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 323 Antibodies
Functional Studies (Func) 68 Antibodies
Immunocytochemistry (ICC) 106 Antibodies
Immunofluorescence (IF) 118 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 47 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 139 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antibodies
Immunoprecipitation (IP) 115 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Western Blotting (WB) 113 Antibodies

General

Antigen CD28 Antibodies
Reactivity Human
(444), (374), (106), (72), (44), (34), (26), (26), (23), (23), (12), (6), (2), (1), (1), (1), (1)
Host Rat
(516), (203), (77), (67), (28), (25), (12)
Clonality
Monoclonal
Conjugate This CD28 antibody is un-conjugated
(118), (99), (78), (41), (17), (17), (15), (13), (13), (12), (11), (10), (9), (9), (9), (9), (9), (9), (9), (8), (8), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunohistochemistry (Acetone-fixed) (IHC (af)), Flow Cytometry (FACS)
(748), (275), (183), (132), (123), (118), (117), (114), (89), (66), (22), (13), (8), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity Rat anti Human CD28 antibody, clone YTH913.12 recognizes human CD28, a ~44 kDa single pass type 1 trans-membrane protein expressed as a homodimer on a major subset of human T-cells (Thompson et al. 1989), responsible for activation of these cells via interaction with theTCR. CD28 is involved in the tuning of the T-cell for activation via TCR, lowering the threshold for activation from around 8000 triggered TCRs to approximately 1500 (Viola et al.1996).

CD28 along with CD152, also known as CTLA-4 acts as a co-receptor for the co-stimulatory molecules CD80 and CD86 (Azuma et al. 1993). CD28 offers a positive stimulatory role on ligation of CD80 and CD86 while CTLA-4 offers a negative feedback signal preventing CD28 mediated T-cell activation of CD86 (Krummel et al. 1995).

Rat anti human CD28, clone YTH913.12 has been reported to recognize an epitope of CD28 expressed by NK cells, which is not recognized by other anti human CD28 clones such as 9.3 and CD28.2 (Galea-Lauri et al 1999.) Other reports however have failed to demonstrate CD28 staining on peripheral blood derived KK cells using clone YTH913.12 (Wilson et al. 1999).
Predicted Reactivity Human
Characteristics Rat Anti Human Cd28:Low Endotoxin
Protein Size: 220
Endotoxin Level Low Endotoxin
Immunogen The immunogen for anti-CD28 antibody: human peripheral blood T-cells
Isotype IgG2b

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background Rat Anti Human Cd28: Low Endotoxin
Gene ID 940
NCBI Accession NP_006130
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 106cells in 100 μL.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Handling Advice Avoid repeat freeze-thaw cycles.
Storage -20 °C
Storage Comment Store at -20 °C only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28) (ABIN2164999) anti-CD28 (CD28) antibody
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28) (ABIN2164999) anti-CD28 (CD28) antibody (Image 2)
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28) (ABIN2164999) anti-CD28 (CD28) antibody (Image 3)
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28) (ABIN2164999) anti-CD28 (CD28) antibody (Image 4)
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28) (ABIN2164999) anti-CD28 (CD28) antibody (Image 5)