anti-Mouse (Murine) CD28 antibody for ELISA

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
pTyr218
17
16
15
12
11
11
11
9
8
7
6
4
4
4
3
3
3
3
3
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
495
409
103
70
67
42
41
34
27
23
15
7
3
3
3
2
1
1
1
Host
Rabbit
527
199
91
70
40
29
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
112
96
74
37
21
20
19
19
18
17
17
13
13
13
13
12
12
12
8
7
6
6
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
Application
ELISA, Western Blotting (WB)
780
211
185
160
142
140
133
129
89
77
26
19
13
9
8
6
4
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN1531543
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2000361 ELISA IHC (p) Rabbit IgG AA 1-149 Log in to see Polyclonal 1
1 ABIN5539489 ELISA WB Goat Internal Region Log in to see Polyclonal
1 ABIN793287 ELISA WB Goat AA 137-149, Extracellular Domain Log in to see Polyclonal
1 ABIN3182245 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN2464553 ELISA WB Goat Internal Region Log in to see Polyclonal
1 ABIN1584203 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2883595 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN1452525 ELISA WB Rabbit IgG pTyr218, C-Term Log in to see Polyclonal
1 ABIN2885744 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN1575399 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN226843 FACS ELISA Hamster IgG Log in to see
1 ABIN2561705 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
1 ABIN2592985 ELISA WB Rabbit IgG AA 33-174 Log in to see Polyclonal
1 ABIN5506224 ELISA WB Rabbit IgG AA 1-149 Log in to see Polyclonal
1 ABIN2224852 ELISA WB APC Goat Log in to see Polyclonal
1 ABIN2224853 ELISA WB Biotin Goat Log in to see Polyclonal
1 ABIN2224854 ELISA WB FITC Goat Log in to see Polyclonal
1 ABIN2224856 ELISA WB PE Goat Log in to see Polyclonal
1 ABIN2224851 ELISA WB Alkaline Phosphatase (AP) Goat Log in to see Polyclonal
1 ABIN2224807 ELISA WB Goat Log in to see Polyclonal

General

Antigen CD28 Antibodies
Epitope pTyr218
(17), (16), (15), (12), (11), (11), (11), (9), (8), (7), (6), (4), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(495), (409), (103), (70), (67), (42), (41), (34), (27), (23), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(527), (199), (91), (70), (40), (29), (12), (1)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(112), (96), (74), (37), (21), (20), (19), (19), (18), (17), (17), (13), (13), (13), (13), (12), (12), (12), (8), (7), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Western Blotting (WB)
(780), (211), (185), (160), (142), (140), (133), (129), (89), (77), (26), (19), (13), (9), (8), (6), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity CD28 (Phospho-Tyr218) Antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218.
Isotype IgG

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background Molecular weight of target protein: 25 kDa
Synonyms: SCFR, Proto-oncogene tyrosine-protein kinase Kit, c-kit, CD117 antigen
Molecular Weight 25 kDa
Gene ID 940
OMIM 186760
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes WB: 1:500-1:1000
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.591629 (NCBI Gene Symbol: CD28)

Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 (CD28) (pTyr218) antibody (ABIN1531543) Western blot analysis of extracts from HepG2 cells treated with nocodazole 1ug/ml 16h...