anti-Mouse (Murine) CD28 antibody for ELISA

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
AA 1-149
16
16
15
12
11
11
11
10
8
7
6
4
4
3
3
3
3
3
3
1
1
1
1
1
Reactivity
Mouse (Murine)
479
389
103
70
51
34
27
26
25
22
15
7
3
3
3
2
1
1
1
Host
Rabbit
511
194
90
70
40
29
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
111
94
72
37
20
19
18
18
17
16
16
12
12
12
12
11
10
10
8
7
6
6
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
761
211
185
148
142
140
129
127
89
71
26
13
13
9
8
6
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN2000361
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1531543 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN793287 ELISA WB Goat AA 137-149, Extracellular Domain Log in to see Polyclonal
1 ABIN2464553 ELISA WB Goat Internal Region Log in to see Polyclonal
1 ABIN1584203 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN3182245 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN1452525 ELISA WB Rabbit IgG pTyr218, C-Term Log in to see Polyclonal
1 ABIN2883595 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN2592985 ELISA WB Rabbit IgG AA 33-174 Log in to see Polyclonal
1 ABIN2561705 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
1 ABIN2885744 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN5506224 ELISA WB Rabbit IgG AA 1-149 Log in to see Polyclonal
1 ABIN226843 FACS ELISA Hamster IgG Log in to see
1 ABIN2224853 ELISA WB Biotin Goat Log in to see Polyclonal
1 ABIN2224856 ELISA WB PE Goat Log in to see Polyclonal
1 ABIN2224854 ELISA WB FITC Goat Log in to see Polyclonal
1 ABIN2224851 ELISA WB Alkaline Phosphatase (AP) Goat Log in to see Polyclonal
1 ABIN2224852 ELISA WB APC Goat Log in to see Polyclonal
1 ABIN1575399 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN2224807 ELISA WB Goat Log in to see Polyclonal
1 ABIN2213288 ELISA Rabbit IgG AA 1-149 Log in to see Polyclonal

Similar anti-CD28 Antibodies

Application / Reactivity Mouse (Murine)
Activation (Act) 2 Antibodies
Agonist (Agon) 1 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA 27 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 301 Antibodies
Functional Studies (Func) 21 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 25 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 58 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 30 Antibodies

General

Antigen CD28 Antibodies
Epitope AA 1-149
(16), (16), (15), (12), (11), (11), (11), (10), (8), (7), (6), (4), (4), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(479), (389), (103), (70), (51), (34), (27), (26), (25), (22), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(511), (194), (90), (70), (40), (29), (12), (1)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(111), (94), (72), (37), (20), (19), (18), (18), (17), (16), (16), (12), (12), (12), (12), (11), (10), (10), (8), (7), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(761), (211), (185), (148), (142), (140), (129), (127), (89), (71), (26), (13), (13), (9), (8), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling ProductDetails: References for anti-CD28 antibody (ABIN2000361) Images
Specificity Mouse CD28
Characteristics Produced in rabbits immunized with purified, recombinant Mouse CD28 (rM CD28, ABIN2007331 NP_031668.3, Met 1-Lys 149,. CD28 specific IgG was purified by Mouse CD28 affinity chromatography.
Purification purified by affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Mouse CD28 protein (ABIN2007331)
Isotype IgG

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling ProductDetails: References for anti-CD28 antibody (ABIN2000361) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28,(Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
NCBI Accession NP_031668.3
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling ProductDetails: References for anti-CD28 antibody (ABIN2000361) Images back to top
Application Notes ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CD28. The detection limit for Mouse CD28 is approximately 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details ProductDetails: References for anti-CD28 antibody (ABIN2000361) Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

ProductDetails: References for anti-CD28 antibody (ABIN2000361)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Keir, Sharpe: "The B7/CD28 costimulatory family in autoimmunity." in: Immunological reviews, Vol. 204, pp. 128-43, 2005

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling ProductDetails: References for anti-CD28 antibody (ABIN2000361) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD28 antibody (CD28) (AA 1-149) (ABIN2000361) Immunochemical staining of mouse CD28 in mouse spleen with rabbit polyclonal antibody...