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anti-Mouse (Murine) CD28 antibody for ELISA

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
pTyr218
21
16
16
15
11
11
11
9
6
4
4
4
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
466
406
106
72
44
34
26
26
24
24
12
6
1
1
1
1
Host
Rabbit
523
214
83
72
40
28
12
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
120
99
78
38
21
20
17
16
16
15
14
12
12
12
12
11
10
10
10
7
6
6
5
4
4
4
4
3
2
2
2
2
2
2
2
2
2
Application
ELISA, Western Blotting (WB)
784
213
181
142
140
135
133
126
97
67
26
13
13
8
6
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN1531543
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2464553 ELISA WB Goat Internal Region Log in to see Polyclonal
1 ABIN793287 ELISA WB Goat Internal Region Log in to see Polyclonal
1 ABIN2000361 ELISA Rabbit IgG AA 1-149 Log in to see Polyclonal 1
1 ABIN1584203 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN3182245 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN1452525 ELISA WB Rabbit IgG pTyr218, C-Term Log in to see Polyclonal
1 ABIN226843 FACS ELISA Hamster IgG Log in to see
1 ABIN2561705 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
1 ABIN2592985 ELISA Rabbit IgG AA 33-174 Log in to see Polyclonal
1 ABIN2224853 ELISA WB Biotin Goat Log in to see Polyclonal
1 ABIN2224854 ELISA WB FITC Goat Log in to see Polyclonal
1 ABIN2224851 ELISA WB Alkaline Phosphatase (AP) Goat Log in to see Polyclonal
1 ABIN2224856 ELISA WB PE Goat Log in to see Polyclonal
1 ABIN2224852 ELISA WB APC Goat Log in to see Polyclonal
1 ABIN1575399 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN2224807 ELISA WB Goat Log in to see Polyclonal
1 ABIN2224855 ELISA WB HRP Goat Log in to see Polyclonal
1 ABIN2136363 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN2649466 ELISA Rabbit Log in to see Polyclonal
1 ABIN2213288 ELISA Rabbit Log in to see Polyclonal

Top referenced anti-CD28 antibody for ELISA

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Application / Reactivity Mouse (Murine)
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Blocking Reagent (BR) 3 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA 21 Antibodies
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Immunofluorescence (IF) 26 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
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Immunohistochemistry (Frozen Sections) (IHC (fro)) 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies

General

Antigen CD28 Antibodies
Epitope pTyr218
(21), (16), (16), (15), (11), (11), (11), (9), (6), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(466), (406), (106), (72), (44), (34), (26), (26), (24), (24), (12), (6), (1), (1), (1), (1)
Host Rabbit
(523), (214), (83), (72), (40), (28), (12)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(120), (99), (78), (38), (21), (20), (17), (16), (16), (15), (14), (12), (12), (12), (12), (11), (10), (10), (10), (7), (6), (6), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Western Blotting (WB)
(784), (213), (181), (142), (140), (135), (133), (126), (97), (67), (26), (13), (13), (8), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity CD28 (Phospho-Tyr218) Antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218.
Isotype IgG

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background Molecular weight of target protein: 25 kDa
Synonyms: SCFR, Proto-oncogene tyrosine-protein kinase Kit, c-kit, CD117 antigen
Molecular Weight 25 kDa
Gene ID 940
OMIM 186760
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes WB: 1:500-1:1000
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.591629 (NCBI Gene Symbol: CD28)

Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 antibody (CD28) (pTyr218) (ABIN1531543) Western blot analysis of extracts from HepG2 cells treated with nocodazole 1ug/ml 16h...