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anti-Mouse (Murine) CD28 antibody for Flow Cytometry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Mouse (Murine)
442
362
106
69
44
31
26
23
23
23
12
6
2
1
1
1
1
Host
Hamster
512
202
77
66
25
23
7
Clonality
Monoclonal
Conjugate
This CD28 antibody is conjugated to FITC
115
95
75
41
17
17
15
13
12
12
11
10
9
9
9
9
9
9
9
8
8
8
6
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS)
733
265
178
132
122
118
116
111
86
66
22
13
4
3
3
3
3
3
2
2
1
1
1
1
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Catalog No. ABIN370907
€ 319.52
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30402437 ABIN2659481 FACS ICFC PE-Cy7 Mouse IgG2b kappa Log in to see E18 3
0.30402437 ABIN2661640 FACS FITC Mouse IgG2b kappa Log in to see E18 4
0.30402437 ABIN2657375 FACS ICFC Alexa Fluor 488 Mouse IgG2b kappa Log in to see E18 3
0.30402437 ABIN2660334 Act FACS IHC PerCP-Cy5.5 Hamster IgG Log in to see 37-51 9
0.30402437 ABIN2663302 FACS PE Hamster IgG Log in to see 37-51 9
0.30402437 ABIN2663308 FACS PE Mouse IgG2b kappa Log in to see E18 4
0.30402437 ABIN370903 FACS PE Hamster IgG Log in to see 37-51 8
0.30402437 ABIN2665914 FACS Hamster IgG Log in to see 37-51 9
0.30402437 ABIN2665972 FACS IHC Hamster IgG Log in to see 37-51 9
0.30402437 ABIN254772 FACS Biotin Golden Syrian Hamster IgG Log in to see 37-51-1
0.30402437 ABIN254773 FACS FITC Golden Syrian Hamster IgG Log in to see 37-51-1
0.30402437 ABIN2479673 FACS FITC Hamster IgG Log in to see 37-51-1 2
0.30402437 ABIN2665487 FACS Hamster IgG Log in to see 37-51 16
0.30402437 ABIN439924 FACS in vitro IP Biotin Armenian Hamster IgG Log in to see PV-1
0.30402437 ABIN439926 FACS in vitro IP Allophycocyanin Armenian Hamster IgG Log in to see PV-1
0.30402437 ABIN439920 FACS in vitro IP PE Golden Syrian Hamster IgG Log in to see 37-51
0.30402437 ABIN439923 FACS in vitro IP FITC Armenian Hamster IgG Log in to see PV-1
0.30402437 ABIN439925 FACS in vitro IP PE Armenian Hamster IgG Log in to see PV-1
0.30402437 ABIN2167181 FACS IP Hamster IgG Log in to see
0.30402437 ABIN2168775 FACS FITC Hamster IgG Log in to see Polyclonal

Top referenced anti-CD28 antibody for Flow Cytometry

Similar anti-CD28 Antibodies

Application / Reactivity Mouse (Murine)
Activation (Act) 2 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA 22 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 287 Antibodies
Functional Studies (Func) 27 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 91 Antibodies
In vitro Assay (in vitro) 7 Antibodies
Intracellular Flow Cytometry (ICFC) 9 Antibodies

General

Antigen CD28 Antibodies
Reactivity Mouse (Murine)
(442), (362), (106), (69), (44), (31), (26), (23), (23), (23), (12), (6), (2), (1), (1), (1), (1)
Host Hamster
(512), (202), (77), (66), (25), (23), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is conjugated to FITC
(115), (95), (75), (41), (17), (17), (15), (13), (12), (12), (11), (10), (9), (9), (9), (9), (9), (9), (9), (8), (8), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS)
(733), (265), (178), (132), (122), (118), (116), (111), (86), (66), (22), (13), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN370907) Images
Characteristics Synonyms: TP44, T-cell-specific surface glycoprotein CD28
Clone PV-1
Isotype IgG

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN370907) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. (Ref.1-4) CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. (Ref.5) CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses. (Ref.6-9)Synonyms: T-cell-specific surface glycoprotein CD28, TP44
UniProt P31041
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN370907) Images back to top
Application Notes Flow Cytometry: < / = 3 μg/10^6 cells. (Ref.8-10)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN370907) Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CD28 Antibody (ABIN370907)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Gelfanov, Lai, Gelfanova, Dong, Su, Liao: "Differential requirement of CD28 costimulation for activation of murine CD8+ intestinal intraepithelial lymphocyte subsets and lymph node cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 76-82, 1995

Abe, Vandenberghe, Craighead, Smoot, Lee, June: "Distinct signal transduction in mouse CD4+ and CD8+ splenic T cells after CD28 receptor ligation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 3, pp. 985-97, 1995

Levine, Ueda, Craighead, Huang, June: "CD28 ligands CD80 (B7-1) and CD86 (B7-2) induce long-term autocrine growth of CD4+ T cells and induce similar patterns of cytokine secretion in vitro." in: International immunology, Vol. 7, Issue 6, pp. 891-904, 1995

Nandi, Gross, Allison: "CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3361-9, 1994

Clements, Winslow, Donahue, Cooper, Allison, Budd: "Co-stimulation via CD28 induces activation of a refractory subset of MRL-lpr/lpr T lymphocytes." in: International immunology, Vol. 5, Issue 11, pp. 1451-60, 1994

Giese, Allison, Davidson: "Functionally anergic lpr and gld B220+ T cell receptor (TCR)-alpha/beta+ double-negative T cells express CD28 and respond to costimulation with phorbol myristate acetate and antibodies to CD28 and the TCR." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 2, pp. 597-609, 1993

Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, 1992

Harding, McArthur, Gross, Raulet, Allison: "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." in: Nature, Vol. 356, Issue 6370, pp. 607-9, 1992

June, Ledbetter, Linsley, Thompson: "Role of the CD28 receptor in T-cell activation." in: Immunology today, Vol. 11, Issue 6, pp. 211-6, 1990

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN370907) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28)  (FITC) (ABIN370907) anti-CD28 (CD28) antibody (FITC)