We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Mouse (Murine)|
|Activation (Act)||2 Antibodies|
|Blocking Antibody (Inhibition)||1 Antibodies|
|Blocking Reagent (BR)||4 Antibodies|
|Cytotoxicity Test (CyTox)||3 Antibodies|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||1 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||238 Antibodies|
|Functional Studies (Func)||27 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies|
|Immunofluorescence (IF)||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||9 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies|
|Immunoprecipitation (IP)||91 Antibodies|
|In vitro Assay (in vitro)||7 Antibodies|
|Intracellular Flow Cytometry (ICFC)||9 Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD28 antibody is conjugated to FITC Alternatives|
Flow Cytometry (FACS)
|9 references available|
|Supplier||Log in to see|
Product Details anti-CD28 AntibodyTarget Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN370907) Images
|Characteristics||Synonyms: TP44, T-cell-specific surface glycoprotein CD28|
Target Details CD28Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN370907) Images back to top
|Alternative Name||CD28 (CD28 Antibody Abstract)|
|Background||CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. (Ref.1-4) CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. (Ref.5) CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses. (Ref.6-9)Synonyms: T-cell-specific surface glycoprotein CD28, TP44|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway|
Application DetailsProduct Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN370907) Images back to top
Flow Cytometry: < / = 3 μg/10^6 cells. (Ref.8-10)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN370907) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CD28 Antibody (ABIN370907)Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Clements, Winslow, Donahue et al.: "Co-stimulation via CD28 induces activation of a refractory subset of MRL-lpr/lpr T lymphocytes." in: International immunology, Vol. 5, Issue 11, pp. 1451-60, 1994 (PubMed).
Abe, Vandenberghe, Craighead et al.: "Distinct signal transduction in mouse CD4+ and CD8+ splenic T cells after CD28 receptor ligation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 3, pp. 985-97, 1995 (PubMed).
Giese, Allison, Davidson: "Functionally anergic lpr and gld B220+ T cell receptor (TCR)-alpha/beta+ double-negative T cells express CD28 and respond to costimulation with phorbol myristate acetate and antibodies to CD28 and the TCR." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 2, pp. 597-609, 1993 (PubMed).
Gelfanov, Lai, Gelfanova et al.: "Differential requirement of CD28 costimulation for activation of murine CD8+ intestinal intraepithelial lymphocyte subsets and lymph node cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 76-82, 1995 (PubMed).
Levine, Ueda, Craighead et al.: "CD28 ligands CD80 (B7-1) and CD86 (B7-2) induce long-term autocrine growth of CD4+ T cells and induce similar patterns of cytokine secretion in vitro." in: International immunology, Vol. 7, Issue 6, pp. 891-904, 1995 (PubMed).
Nandi, Gross, Allison: "CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3361-9, 1994 (PubMed).
June, Ledbetter, Linsley et al.: "Role of the CD28 receptor in T-cell activation." in: Immunology today, Vol. 11, Issue 6, pp. 211-6, 1990 (PubMed).
Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, 1992 (PubMed).
Harding, McArthur, Gross et al.: "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." in: Nature, Vol. 356, Issue 6370, pp. 607-9, 1992 (PubMed).
ImagesProduct Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN370907) back to top