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anti-Primate CD28 antibody for Flow Cytometry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Reactivity
Human, Primate
466
384
101
70
49
34
27
25
24
21
15
6
2
2
2
2
1
1
Host
Mouse
501
190
89
70
40
26
12
1
Clonality
Monoclonal
Conjugate
This CD28 antibody is conjugated to FITC
109
90
72
36
20
18
18
17
17
16
16
12
12
12
12
11
10
10
8
7
6
6
6
6
6
5
4
3
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS)
746
208
184
145
140
135
129
127
89
72
26
13
13
9
8
6
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN400892
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.770191 ABIN400889 FACS APC Mouse IgG1 Log in to see CD28-2
12.770191 ABIN400893 FACS PE Mouse IgG1 Log in to see CD28-2
12.770191 ABIN400890 Func FACS IHC (fro) IF IP WB Mouse IgG1 Log in to see CD28-2
12.770191 ABIN781619 Func IHC (fro) FACS IF IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN490197 FACS APC Mouse IgG1 Log in to see CD28-2
1 ABIN490224 FACS FITC Mouse IgG1 Log in to see CD28-2
1 ABIN490256 FACS PE Mouse IgG1 Log in to see CD28-2
1 ABIN490298 FACS PerCP Mouse IgG1 Log in to see CD28-2
1 ABIN1959966 Func ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN4291333 FACS IF PE Mouse IgG2a kappa Log in to see 204-12
1 ABIN4291200 CyTOF FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN1967135 Func ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN4291340 FACS DyLight 680 Mouse IgG2a kappa Log in to see 204-12
1 ABIN4291341 FACS DyLight 488 Mouse IgG2a kappa Log in to see 204-12
1 ABIN4291343 FACS DyLight 755 Mouse IgG2a kappa Log in to see 204-12
1 ABIN4291344 FACS PerCP Mouse IgG2a kappa Log in to see 204-12
1 ABIN4291346 FACS DyLight 350 Mouse IgG2a kappa Log in to see 204-12
1 ABIN4291350 FACS FITC Mouse IgG2a kappa Log in to see 204-12
1 ABIN4291345 FACS DyLight 550 Mouse IgG2a kappa Log in to see 204-12
1 ABIN4291347 FACS DyLight 405 Mouse IgG2a kappa Log in to see 204-12

Similar anti-CD28 Antibodies

Application / Reactivity Human Primate
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies 1 Antibodies
Western Blotting (WB) 113 Antibodies 19 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Immunoprecipitation (IP) 53 Antibodies 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunohistochemistry (IHC) 62 Antibodies 15 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 127 Antibodies 19 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunofluorescence (IF) 127 Antibodies 21 Antibodies

General

Antigen CD28 Antibodies
Reactivity Human, Primate
(466), (384), (101), (70), (49), (34), (27), (25), (24), (21), (15), (6), (2), (2), (2), (2), (1), (1)
Host Mouse
(501), (190), (89), (70), (40), (26), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is conjugated to FITC
(109), (90), (72), (36), (20), (18), (18), (17), (17), (16), (16), (12), (12), (12), (12), (11), (10), (10), (8), (7), (6), (6), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS)
(746), (208), (184), (145), (140), (135), (129), (127), (89), (72), (26), (13), (13), (9), (8), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity The antibody CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
Cross-Reactivity (Details) Species reactivity (tested):Human, Primates
Characteristics Synonyms: TP44, T-cell-specific surface glycoprotein CD28
Immunogen DC28. 1. 3. 3 hybridoma cells
Clone CD28-2
Isotype IgG1

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
Gene ID 940
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes Flow cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole bloodor 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Buffer Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28)  (FITC) (ABIN400892) anti-CD28 (CD28) antibody (FITC)