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|Application / Reactivity||Human||Primate|
|Immunofluorescence (fixed cells) (IF/ICC)||7 Antibodies|
|Cytometry by Time of Flight (CyTOF)||10 Antibodies||1 Antibodies|
|Western Blotting (WB)||113 Antibodies||19 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Intracellular Flow Cytometry (ICFC)||1 Antibodies|
|Immunoprecipitation (IP)||53 Antibodies||6 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||48 Antibodies|
|Immunohistochemistry (IHC)||62 Antibodies||15 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||127 Antibodies||19 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||26 Antibodies|
|Immunofluorescence (IF)||127 Antibodies||21 Antibodies|
|Reactivity||Human, Primate Alternatives|
|Conjugate||This CD28 antibody is conjugated to FITC Alternatives|
Flow Cytometry (FACS)
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Product Details anti-CD28 AntibodyTarget Details CD28 Application Details Handling Images
|Specificity||The antibody CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Primates|
|Characteristics||Synonyms: TP44, T-cell-specific surface glycoprotein CD28|
|Immunogen||DC28. 1. 3. 3 hybridoma cells|
Target Details CD28Product Details anti-CD28 Antibody Application Details Handling Images back to top
|Alternative Name||CD28 (CD28 Antibody Abstract)|
|Background||CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Synonyms: T-cell-specific surface glycoprotein CD28, TP44|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Flow cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole bloodor 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top