You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Primate CD28 antibody for Flow Cytometry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Human, Primate
444
374
106
72
43
34
26
26
23
23
12
6
2
1
1
1
1
Host
Mouse
515
203
77
68
28
25
12
Clonality
Monoclonal
Conjugate
This CD28 antibody is conjugated to APC
118
99
78
40
17
17
15
13
13
12
11
10
9
9
9
9
9
9
9
8
8
8
6
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS)
748
275
183
132
123
118
117
114
89
66
22
13
8
4
3
3
3
3
3
2
2
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN400889
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3087334 ABIN400893 FACS PE Mouse IgG1 Log in to see CD28-2
0.3087334 ABIN400892 FACS FITC Mouse IgG1 Log in to see CD28-2
0.3087334 ABIN400890 Func FACS IHC (fro) IF IP WB Mouse IgG1 Log in to see CD28-2
0.3087334 ABIN781619 Func IHC (fro) FACS IF IP WB Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291200 FACS Func ICC IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291333 FACS IF PE Mouse IgG2a kappa Log in to see 204-12
0.0010452945 ABIN4291313 FACS Func ICC IF IHC IHC (fro) IP WB Alexa Fluor 405 Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291314 FACS Func ICC IF IHC IHC (fro) IP WB Alexa Fluor 488 Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291315 FACS Func ICC IF IHC IHC (fro) IP WB Alexa Fluor 647 Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291317 FACS Func ICC IF IHC IHC (fro) IP WB Allophycocyanin Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291316 FACS Func ICC IF IHC IHC (fro) IP WB Alexa Fluor 700 Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291318 FACS Func ICC IF IHC IHC (fro) IP WB Biotin Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291319 FACS Func ICC IF IHC IHC (fro) IP WB DyLight 650 Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291321 FACS Func ICC IF IHC IHC (fro) IP WB DyLight 488 Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291320 FACS Func ICC IF IHC IHC (fro) IP WB DyLight 680 Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291324 FACS Func ICC IF IHC IHC (fro) IP WB PerCP Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291323 FACS Func ICC IF IHC IHC (fro) IP WB DyLight 755 Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291325 FACS Func ICC IF IHC IHC (fro) IP WB PE Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291327 FACS Func ICC IF IHC IHC (fro) IP WB DyLight 350 Mouse IgG1 Log in to see CD28-2
0.0010452945 ABIN4291326 FACS Func ICC IF IHC IHC (fro) IP WB DyLight 550 Mouse IgG1 Log in to see CD28-2

Similar anti-CD28 Antibodies

Application / Reactivity Human Primate
Western Blotting (WB) 113 Antibodies 20 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Immunoprecipitation (IP) 115 Antibodies 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antibodies
Immunohistochemistry (IHC) 47 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 139 Antibodies 20 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunofluorescence (IF) 118 Antibodies 24 Antibodies
Immunocytochemistry (ICC) 106 Antibodies 18 Antibodies
Functional Studies (Func) 68 Antibodies

General

Antigen CD28 Antibodies
Reactivity Human, Primate
(444), (374), (106), (72), (43), (34), (26), (26), (23), (23), (12), (6), (2), (1), (1), (1), (1)
Host Mouse
(515), (203), (77), (68), (28), (25), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is conjugated to APC
(118), (99), (78), (40), (17), (17), (15), (13), (13), (12), (11), (10), (9), (9), (9), (9), (9), (9), (9), (8), (8), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS)
(748), (275), (183), (132), (123), (118), (117), (114), (89), (66), (22), (13), (8), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity The antibody reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
Cross-Reactivity (Details) Species reactivity (tested):Human, Non-Human Primates.
Characteristics Synonyms: TP44, T-cell-specific surface glycoprotein CD28
Immunogen DC28. 1. 3. 3 hybridoma cells
Clone CD28-2
Isotype IgG1

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
Gene ID 940
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes Flow Cytometry analysis of human blood cells using 10 μL reagent / 100 μL of whole bloodor 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Buffer PBS containing 15 mM Sodium Azide as preservative and 0.2 % (w/v) high-grade protease free BSA as a stabilizing agent.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28)  (APC) (ABIN400889) anti-CD28 (CD28) antibody (APC)