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anti-Primate CD28 antibody for Flow Cytometry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Human, Primate
314
306
102
38
26
12
6
5
4
2
2
2
1
1
1
1
Host
Mouse
359
189
77
42
25
20
7
Clonality
Monoclonal
Conjugate
This CD28 antibody is conjugated to APC
106
90
66
40
10
8
7
7
7
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS)
540
174
114
95
92
43
41
34
27
22
13
8
4
3
3
3
3
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN400889
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3068567 ABIN400892 FACS FITC Mouse IgG1 Log in to see CD28-2
0.3068567 ABIN400893 FACS PE Mouse IgG1 Log in to see CD28-2
0.3068567 ABIN400890 Func FACS IHC (fro) IF IP WB Mouse IgG1 Log in to see CD28-2
0.3068567 ABIN781619 Func IHC (fro) FACS IF IP WB Mouse IgG1 Log in to see CD28-2

Similar anti-CD28 Antibodies

Application / Reactivity Human Primate
Western Blotting (WB) 95 Antibodies 2 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Immunoprecipitation (IP) 37 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 35 Antibodies 2 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunofluorescence (IF) 30 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Functional Studies (Func) 44 Antibodies 2 Antibodies

General

Antigen CD28 Antibodies
Reactivity Human, Primate
(314), (306), (102), (38), (26), (12), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(359), (189), (77), (42), (25), (20), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is conjugated to APC
(106), (90), (66), (40), (10), (8), (7), (7), (7), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS)
(540), (174), (114), (95), (92), (43), (41), (34), (27), (22), (13), (8), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity The antibody reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
Cross-Reactivity (Details) Species reactivity (tested):Human, Non-Human Primates.
Characteristics Synonyms: TP44, T-cell-specific surface glycoprotein CD28
Immunogen DC28. 1. 3. 3 hybridoma cells
Clone CD28-2
Isotype IgG1

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
Gene ID 940
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes Flow Cytometry analysis of human blood cells using 10 μL reagent / 100 μL of whole bloodor 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Buffer PBS containing 15 mM Sodium Azide as preservative and 0.2 % (w/v) high-grade protease free BSA as a stabilizing agent.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28)  (APC) (ABIN400889) anti-CD28 (CD28) antibody (APC)