You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Rat (Rattus) CD28 antibody for Immunoprecipitation

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Rat (Rattus)
445
374
105
72
44
34
26
26
23
23
12
6
2
1
1
1
1
Host
Mouse
515
203
77
68
28
25
12
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
118
99
78
41
17
17
15
13
13
12
11
10
9
9
9
9
9
9
9
8
8
8
6
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
748
274
183
132
123
118
116
114
89
66
22
13
8
4
3
3
3
3
3
2
2
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN120439
$ 588.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN951274 FACS IP Mouse IgG1 Log in to see JJ319 1
0.00089556724 ABIN120440 FACS IP Mouse IgG1 Log in to see JJ319 1
0.00089556724 ABIN611634 Func FACS IP Mouse IgG1 Log in to see JJ319 9
0.00089556724 ABIN1177272 FACS IP Mouse IgG1, kappa Log in to see JJ319 4
0.00089556724 ABIN1177271 FACS IP Mouse IgG1, kappa Log in to see JJ316 3
0.00089556724 ABIN133303 FACS IP Mouse IgG1 Log in to see JJ319
0.00089556724 ABIN133304 FACS IP Mouse IgG1 Log in to see JJ319
0.00089556724 ABIN475534 FACS IP Mouse IgG1 Log in to see JJ319
0.00089556724 ABIN1821162 FACS IP Mouse IgG1, kappa Log in to see G1-G1-4
0.00089556724 ABIN2689146 FACS IP Mouse IgG1, kappa Log in to see JJ319 2
0.00089556724 ABIN2749055 Func FACS IP FITC Mouse IgG1 Log in to see JJ319 9
0.00089556724 ABIN2749056 Func FACS IP PE Mouse IgG1 Log in to see JJ319 8
0.00089556724 ABIN280301 Func FACS IP Mouse IgG1 Log in to see JJ319
0.00089556724 ABIN2164978 FACS IP Mouse IgG1 Log in to see
0.00089556724 ABIN2224795 FACS IP Mouse IgG1 kappa Log in to see JJ319
0.00089556724 ABIN2166627 FACS IP Mouse IgG1 Log in to see
0.00089556724 ABIN2479694 Func FACS IP Mouse IgG1 Log in to see JJ319 2
0.00089556724 ABIN593899 Func FACS IP Mouse IgG1 Log in to see
0.00089556724 ABIN3199702 FACS Func IP PE Mouse IgG1 Log in to see JJ319
0.00089556724 ABIN3199701 FACS Func IP FITC Mouse IgG1 Log in to see JJ319

Top referenced anti-CD28 antibody for Immunoprecipitation

Similar anti-CD28 Antibodies

Application / Reactivity Rat (Rattus)
ELISA 1 Antibodies
Flow Cytometry (FACS) 72 Antibodies
Functional Studies (Func) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Immunoprecipitation (IP) 32 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies
Western Blotting (WB) 9 Antibodies

General

Antigen CD28 Antibodies
Reactivity Rat (Rattus)
(445), (374), (105), (72), (44), (34), (26), (26), (23), (23), (12), (6), (2), (1), (1), (1), (1)
Host Mouse
(515), (203), (77), (68), (28), (25), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(118), (99), (78), (41), (17), (17), (15), (13), (13), (12), (11), (10), (9), (9), (9), (9), (9), (9), (9), (8), (8), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
(748), (274), (183), (132), (123), (118), (116), (114), (89), (66), (22), (13), (8), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN120439) Images
Specificity This antibody recognizes the cell surface antigen CD28. Clone JJ319 has been identified as a highly effective co-stimulator of T cell proliferation and IL-2 secretion by resting T cells.
Purification Affinity Chromatography on Protein G.
Endotoxin Level Low Endotoxin
Immunogen Human PHA blasts.
Clone JJ319
Isotype IgG1

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN120439) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is a type I transmembrane glycoprotein, expressed by the majority of T lymphocytes and a subset of NK cells. CD28 is an essential costimulatory receptor for T cell activation and a modulator of T-cell cytokine production. Ligands of CD28 include CD80 (B7-1), CD86 (B7-2) and P13-kinase.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
Gene ID 25660
NCBI Accession NP_037253
UniProt P31042
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN120439) Images back to top
Application Notes Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN120439) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C

References for anti-CD28 Antibody (ABIN120439)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Tacke, Hanke, Hanke, Hünig: "CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." in: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, 1997

Tacke, Clark, Dallman, Hünig et al.: "Cellular distribution and costimulatory function of rat CD28. Regulated expression during thymocyte maturation and induction of cyclosporin A sensitivity of costimulated T cell responses by phorbol ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 10, pp. 5121-7, 1995