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anti-Baboon CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Baboon, Cynomolgus, Human, Rhesus Monkey
765
404
130
68
28
27
25
20
20
15
14
12
12
11
11
10
10
9
8
8
3
3
2
2
2
1
1
1
1
Host
Mouse
960
391
161
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
243
190
122
86
30
30
27
18
18
17
16
15
13
11
10
10
10
9
8
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1177
282
232
188
136
111
105
81
69
36
33
25
23
17
14
10
7
6
6
6
5
4
4
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2689403
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010650172 ABIN2472483 IHC (fro) FACS Mouse IgG1 Log in to see RPA-T8

Top referenced anti-CD8 antibody for Flow Cytometry

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Application / Reactivity Human Cynomolgus Rhesus Monkey Baboon
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Multiplex Assay (MA) 1 Antibodies
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Mass Cytometry (CyTOF) 6 Antibodies
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Intracellular Flow Cytometry (ICFC) 15 Antibodies
Immunostaining (ISt) 4 Antibodies
Immunoprecipitation (IP) 90 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 52 Antibodies
Immunohistochemistry (IHC) 146 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 97 Antibodies 4 Antibodies 4 Antibodies 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Baboon, Cynomolgus, Human, Rhesus Monkey
(765), (404), (130), (68), (28), (27), (25), (20), (20), (15), (14), (12), (12), (11), (11), (10), (10), (9), (8), (8), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(960), (391), (161)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(243), (190), (122), (86), (30), (30), (27), (18), (18), (17), (16), (15), (13), (11), (10), (10), (10), (9), (8), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1177), (282), (232), (188), (136), (111), (105), (81), (69), (36), (33), (25), (23), (17), (14), (10), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2689403) Images
Brand BD Pharmingen™
Characteristics The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α). CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) or as a heterodimer (CD8αβ) when disulfide-bonded to a CD8 beta chain (CD8β). CD8-positive αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers. CD8 plays important roles in T cell activation and selection. The extracellular IgSF domain of CD8α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling. The RPA-T8 and HIT8a monoclonal antibodies are not cross-blocking. This clone has been reported to react with a subset of peripheral blood lymphocytes, but not monocytes nor granuloyctes, of baboon and both rhesus and cynomolgus macaque monkey. In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are observed in non-human primates compared to normal human donors. Profile of anti-CD8 reactivity on peripheral blood lymphocytes of rhesus macaque (macaca mulatta) analyzed by flow cytometry.

BD Pharmingen™ Purified Mouse Anti-Human CD8 - Purified - Clone RPA-T8 - Isotype Mouse IgG1, κ - Reactivity Hu, Bab, Cyno, Rhe - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Clone RPA
Isotype IgG1, kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN2689403) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background Synonyms: CD8α, CD8A, CD8 alpha, Leu2, MAL, T8, p32
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN2689403) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN2689403) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD8 Antibody (ABIN2689403)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Sopper, Stahl-Hennig, Demuth, Johnston, Dörries, ter Meulen: "Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys." in: Cytometry, Vol. 29, Issue 4, pp. 351-62, 1998