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|Antigen||CD8a Molecule (CD8A) Antibodies|
|Conjugate||This CD8 antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
|2 references available|
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Product Details anti-CD8 AntibodyTarget Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN371338) Images
|Characteristics||Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2|
Target Details CD8Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN371338) Images back to top
|Alternative Name||CD8 (CD8A Antibody Abstract)|
|Background||The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. CD8 is expressed on most thymocytes and approximately 1/3 of peripheral blood T cells in humans. CD8 alpha/beta heterodimers are expressed only on TCR alpha/beta T cells, whereas CD8 alpha homodimers can be expressed on alpha/beta and gamma/delta T cells and some NK cells in humans. The pattern of expression in other species has not been well documented.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN371338) Images back to top
Flow Cytometry: Identification and enumeration of CD8+cells. (Ref.2)Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections only): Identification ofCD8+ T cells. (Ref.4)Immunoprecipitation. (Ref.2)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN371338) Images back to top
|Buffer||100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CD8 Antibody (ABIN371338)Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Tregaskes, Kong, Paramithiotis, Chen, Ratcliffe, Davison, Young et al.: "Identification and analysis of the expression of CD8 alpha beta and CD8 alpha alpha isoforms in chickens reveals a major TCR-gamma delta CD8 alpha beta subset of intestinal intraepithelial ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 9, pp. 4485-94, 1995
Chan, Chen, Ager, Cooper: "Identification of the avian homologues of mammalian CD4 and CD8 antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 7, pp. 2133-8, 1988