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anti-Dog (Canine) CD8 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Dog (Canine)
766
404
130
68
28
27
24
20
20
16
15
12
12
11
11
10
10
9
8
8
3
3
2
2
2
2
1
1
1
Host
Mouse
960
391
161
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
243
190
122
86
30
30
27
18
18
17
16
15
13
11
10
10
10
9
8
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
1177
282
231
187
136
111
105
81
69
36
33
25
23
17
14
10
7
6
6
6
5
4
4
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN181842
$ 566.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008852861 ABIN2472511 IHC (fro) FACS IF Rat IgG1 Log in to see YCATE55-9 4
0.0008852861 ABIN2472528 IHC (fro) FACS IP Mouse IgG2a Log in to see CA9-JD3 4
0.0008852861 ABIN642905 IHC (fro) FACS IP Mouse Log in to see

Top referenced anti-CD8 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-CD8 Antibodies

Application / Reactivity Dog (Canine)
Flow Cytometry (FACS) 24 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunohistochemistry (IHC) 3 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunoprecipitation (IP) 5 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Dog (Canine)
(766), (404), (130), (68), (28), (27), (24), (20), (20), (16), (15), (12), (12), (11), (11), (10), (10), (9), (8), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(960), (391), (161)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(243), (190), (122), (86), (30), (30), (27), (18), (18), (17), (16), (15), (13), (11), (10), (10), (10), (9), (8), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(1177), (282), (231), (187), (136), (111), (105), (81), (69), (36), (33), (25), (23), (17), (14), (10), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN181842) Images
Specificity This antibody recognises Canine homologue CD8 alpha chain. Clone CA9.JD3 has been reported to inhibit cytotoxic T lymphocyte function.
Purification Supernatant
Immunogen Canine Thymocytes. Spleen cells from immunised Balb/c mice were fused with cells of the P3X63-Ag.653 myeloma cell line.
Clone CA9-JD3
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN181842) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background CD8 is expressed by a thymocytes, peripheral T cells and a splenic zone macrophages.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 493799
NCBI Accession NP_001009843
UniProt P41688
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN181842) Images back to top
Application Notes Immunoprecipiation. Flow Cytometry: Neat. Immunohistochemistry on frozen sections: Neat - 1/10. Recommended Positive Control: Spleen, lymph node
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN181842) Images back to top
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD8 Antibody (ABIN181842)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Cobbold, Metcalfe: "Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW)." in: Tissue antigens, Vol. 43, Issue 3, pp. 137-54, 1994

Moore, Rossitto, Danilenko, Wielenga, Raff, Severns: "Monoclonal antibodies specific for canine CD4 and CD8 define functional T-lymphocyte subsets and high-density expression of CD4 by canine neutrophils." in: Tissue antigens, Vol. 40, Issue 2, pp. 75-85, 1992