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|Application / Reactivity||Sheep (Ovine)||Cow (Bovine)||Goat|
|Immunoprecipitation (IP)||4 Antibodies||5 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||3 Antibodies||2 Antibodies||2 Antibodies|
|Immunohistochemistry (IHC)||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies||3 Antibodies||4 Antibodies|
|Immunofluorescence (IF)||4 Antibodies||2 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||10 Antibodies||19 Antibodies||20 Antibodies|
|Antigen||CD8a Molecule (CD8A) Antibodies|
|Reactivity||Cow (Bovine), Goat, Sheep (Ovine) Alternatives|
|Conjugate||This CD8 antibody is conjugated to FITC Alternatives|
Flow Cytometry (FACS)
|5 references available|
|Supplier||Log in to see|
Product Details anti-CD8 AntibodyTarget Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN317440) Images
|Specificity||This antibody reacts with the CD8 antigen expressed by a subset of T lymphocytes. The antibody precipitates molecules of 34 kDa and 34 kDa under reducing conditions.|
|Characteristics||Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
Target Details CD8Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN317440) Images back to top
|Alternative Name||CD8 (CD8A Antibody Abstract)|
|Background||The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN317440) Images back to top
Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN317440) Images back to top
|Buffer||Phosphate buffered saline, 0.09 % Sodium Azide, 1 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. This antibody is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CD8 Antibody (ABIN317440)Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Twizere, Kerkhofs, Burny et al.: "Discordance between bovine leukemia virus tax immortalization in vitro and oncogenicity in vivo." in: Journal of virology, Vol. 74, Issue 21, pp. 9895-902, 2000 (PubMed).
Winkler, Doster, Jones: "Persistence and reactivation of bovine herpesvirus 1 in the tonsils of latently infected calves." in: Journal of virology, Vol. 74, Issue 11, pp. 5337-46, 2000 (PubMed).
Gutierrez, Forster, McConnell et al.: "The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques." in: Veterinary immunology and immunopathology, Vol. 71, Issue 3-4, pp. 321-34, 1999 (PubMed).
Winkler, Doster, Jones: "Bovine herpesvirus 1 can infect CD4(+) T lymphocytes and induce programmed cell death during acute infection of cattle." in: Journal of virology, Vol. 73, Issue 10, pp. 8657-68, 1999 (PubMed).
MacHugh, Sopp: "Individual antigens of cattle. Bovine CD8 (BoCD8)." in: Veterinary immunology and immunopathology, Vol. 27, Issue 1-3, pp. 65-9, 1991 (PubMed).
ImagesProduct Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN317440) back to top