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anti-Human CD8 antibody for Intracellular Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Human
552
471
127
92
42
26
19
13
13
13
12
11
9
4
2
1
1
Host
Mouse
716
435
187
1
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to Alexa Fluor 488
141
138
86
51
44
36
35
32
29
27
24
23
23
23
22
22
18
18
18
18
14
12
11
11
10
9
7
7
7
7
5
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Intracellular Flow Cytometry (ICFC), Immunoprecipitation (IP), Western Blotting (WB)
1080
428
338
242
241
226
185
182
172
25
22
19
16
13
11
11
9
7
6
6
6
6
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2657487
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2658735 FACS ICFC APC Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2659018 ICFC PE-Cy5 Mouse IgG1 kappa Log in to see HIT8a 4
1 ABIN2660733 ICFC IP WB APC-Cy7 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2661843 ICFC FITC Mouse IgG1 kappa Log in to see HIT8a 3
1 ABIN2659916 ICFC PerCP Mouse IgG1 kappa Log in to see RPA-T8 7
1 ABIN2659019 ICFC PE-Cy5 Mouse IgG1 kappa Log in to see HIT8a 4
1 ABIN2658734 FACS ICFC APC Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2657488 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2661844 ICFC FITC Mouse IgG1 kappa Log in to see HIT8a 3
1 ABIN2659917 ICFC PerCP Mouse IgG1 kappa Log in to see RPA-T8 7
1 ABIN2660734 ICFC IP WB APC-Cy7 Mouse IgG1 kappa Log in to see RPA-T8 6

Top referenced anti-CD8 antibody for Intracellular Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 14 Antibodies
ELISA 143 Antibodies
ELISA (Detection) 1 Antibodies
Flow Cytometry (FACS) 384 Antibodies
Functional Studies (Func) 3 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 122 Antibodies
Immunofluorescence (IF) 144 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(552), (471), (127), (92), (42), (26), (19), (13), (13), (13), (12), (11), (9), (4), (2), (1), (1)
Host Mouse
(716), (435), (187), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to Alexa Fluor 488
(141), (138), (86), (51), (44), (36), (35), (32), (29), (27), (24), (23), (23), (23), (22), (22), (18), (18), (18), (18), (14), (12), (11), (11), (10), (9), (7), (7), (7), (7), (5), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Intracellular Flow Cytometry (ICFC), Immunoprecipitation (IP), Western Blotting (WB)
(1080), (428), (338), (242), (241), (226), (185), (182), (172), (25), (22), (19), (16), (13), (11), (11), (9), (7), (6), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2657487) Images
Cross-Reactivity Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Pigtailed Macaque, Sooty mangabey (Cercocebus atys)
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Clone RPA-T8
Isotype IgG1 kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN2657487) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Background CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN2657487) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN2657487) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

References for anti-CD8 Antibody (ABIN2657487)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Rout, Else, Yue, Connole, Exley, Kaur: "Paucity of CD4+ natural killer T (NKT) lymphocytes in sooty mangabeys is associated with lack of NKT cell depletion after SIV infection." in: PLoS ONE, Vol. 5, Issue 3, pp. e9787, 2010

Kmieciak, Gowda, Graham, Godder, Bear, Marincola, Manjili: "Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function." in: Journal of translational medicine, Vol. 7, pp. 89, 2009

Thakral, Dobbins, Devine, Kavathas: "Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 11, pp. 7431-42, 2008

Magidovich, Orr, Fass, Abdu, Yifrach: "Intrinsic disorder in the C-terminal domain of the Shaker voltage-activated K+ channel modulates its interaction with scaffold proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 32, pp. 13022-7, 2007

Mack, Tucker, Sokol, Karrer, Kotzin, Whitington, Miller: "Biliary atresia is associated with CD4+ Th1 cell-mediated portal tract inflammation." in: Pediatric research, Vol. 56, Issue 1, pp. 79-87, 2004

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2657487) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (Alexa Fluor 488) (ABIN2657487) anti-CD8a Molecule (CD8A) antibody (Alexa Fluor 488)