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anti-Human CD8 antibody for Intracellular Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Human
765
404
130
68
28
27
25
20
20
16
15
12
12
11
11
10
10
9
8
8
3
3
2
2
2
2
1
1
1
Host
Mouse
960
391
161
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to Biotin
243
190
121
86
30
30
27
18
18
17
16
15
13
11
10
10
10
9
8
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC)
1178
282
232
188
136
111
105
81
69
36
33
25
22
17
14
10
7
6
6
6
5
4
4
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2660849
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008704084 ABIN2659018 ICFC PE-Cy5 Mouse IgG1 kappa Log in to see HIT8a 4
0.0008704084 ABIN2660733 ICFC IP WB APC-Cy7 Mouse IgG1 kappa Log in to see RPA-T8 6
0.0008704084 ABIN2661843 ICFC FITC Mouse IgG1 kappa Log in to see HIT8a 3
0.0008704084 ABIN2657487 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
0.0008704084 ABIN2658716 FACS ICFC APC Mouse IgG1 kappa Log in to see SK1 7
0.0008704084 ABIN2658735 FACS ICFC APC Mouse IgG1 kappa Log in to see RPA-T8 6
0.0008704084 ABIN2659916 ICFC PerCP Mouse IgG1 kappa Log in to see RPA-T8 7
0.0008704084 ABIN2659019 ICFC PE-Cy5 Mouse IgG1 kappa Log in to see HIT8a 4
0.0008704084 ABIN2660734 ICFC IP WB APC-Cy7 Mouse IgG1 kappa Log in to see RPA-T8 6
0.0008704084 ABIN2661844 ICFC FITC Mouse IgG1 kappa Log in to see HIT8a 3
0.0008704084 ABIN2658717 FACS ICFC APC Mouse IgG1 kappa Log in to see SK1 7
0.0008704084 ABIN2658734 FACS ICFC APC Mouse IgG1 kappa Log in to see RPA-T8 6
0.0008704084 ABIN2657488 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
0.0008704084 ABIN2659917 ICFC PerCP Mouse IgG1 kappa Log in to see RPA-T8 7

Top referenced anti-CD8 antibody for Intracellular Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Human
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Blocking Reagent (BR) 4 Antibodies
Co-Stimulation (CoSt) 2 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
ELISA 97 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 560 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 42 Antibodies
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Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
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Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(765), (404), (130), (68), (28), (27), (25), (20), (20), (16), (15), (12), (12), (11), (11), (10), (10), (9), (8), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(960), (391), (161)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to Biotin
(243), (190), (121), (86), (30), (30), (27), (18), (18), (17), (16), (15), (13), (11), (10), (10), (10), (9), (8), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(1178), (282), (232), (188), (136), (111), (105), (81), (69), (36), (33), (25), (22), (17), (14), (10), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2660849) Images
Cross-Reactivity Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops), Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus Monkey, Sooty mangabey (Cercocebus atys), Green Monkey (Chlorocebus sabaeus)
Purification The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Clone SK1
Isotype IgG1 kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN2660849) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN2660849) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN2660849) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD8 Antibody (ABIN2660849)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Chen, Tsau, Molkentin, Komatsu, Hedrick: "Mechanisms of necroptosis in T cells." in: The Journal of experimental medicine, Vol. 208, Issue 4, pp. 633-41, 2011

Wooldridge, van den Berg, Glick, Gostick, Laugel, Hutchinson, Milicic, Brenchley, Douek, Price, Sewell: "Interaction between the CD8 coreceptor and major histocompatibility complex class I stabilizes T cell receptor-antigen complexes at the cell surface." in: The Journal of biological chemistry, Vol. 280, Issue 30, pp. 27491-501, 2005

Ahmed, Ohtani, Nio, Funaki, Shimaoka, Nagura, Ohi: "CD8+ T cells infiltrating into bile ducts in biliary atresia do not appear to function as cytotoxic T cells: a clinicopathological analysis." in: The Journal of pathology, Vol. 193, Issue 3, pp. 383-9, 2001

Campanelli, Palermo, Garbelli, Mantovani, Lucchi, Necker, Lantelme, Giachino: "Human CD8 co-receptor is strictly involved in MHC-peptide tetramer-TCR binding and T cell activation." in: International immunology, Vol. 14, Issue 1, pp. 39-44, 2001

Carbone, Gloghini, Larocca, Antinori, Falini, Tirelli, Dalla-Favera, Gaidano: "Human immunodeficiency virus-associated Hodgkin's disease derives from post-germinal center B cells." in: Blood, Vol. 93, Issue 7, pp. 2319-26, 1999

Ledbetter, Evans, Lipinski, Cunningham-Rundles, Good, Herzenberg: "Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man." in: The Journal of experimental medicine, Vol. 153, Issue 2, pp. 310-23, 1981

Evans, Wall, Platsoucas, Siegal, Fikrig, Testa, Good: "Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, Issue 1, pp. 544-8, 1981

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2660849) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (Biotin) (ABIN2660849) anti-CD8a Molecule (CD8A) antibody (Biotin)