anti-Human CD8 antibody for Intracellular Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Human
617
525
127
92
44
26
22
19
13
13
13
12
9
5
5
5
4
4
4
2
2
1
1
Host
Mouse
790
488
198
2
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to FITC
150
149
93
60
48
41
40
36
31
29
24
23
23
23
23
22
20
19
19
18
15
13
11
11
11
11
10
9
9
8
7
7
7
4
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC)
1206
446
365
257
248
236
200
185
182
39
35
19
16
13
11
11
9
7
6
6
6
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2661843
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2659018 ICFC PE-Cy5 Mouse IgG1 kappa Log in to see HIT8a 4
1 ABIN2659916 ICFC PerCP Mouse IgG1 kappa Log in to see RPA-T8 7
1 ABIN2660733 ICFC IP WB APC-Cy7 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2657487 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2658735 FACS ICFC APC Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2661844 ICFC FITC Mouse IgG1 kappa Log in to see HIT8a 3
1 ABIN2659019 ICFC PE-Cy5 Mouse IgG1 kappa Log in to see HIT8a 4
1 ABIN2659917 ICFC PerCP Mouse IgG1 kappa Log in to see RPA-T8 7
1 ABIN2660734 ICFC IP WB APC-Cy7 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2657488 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2658734 FACS ICFC APC Mouse IgG1 kappa Log in to see RPA-T8 6

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(617), (525), (127), (92), (44), (26), (22), (19), (13), (13), (13), (12), (9), (5), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host Mouse
(790), (488), (198), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to FITC
(150), (149), (93), (60), (48), (41), (40), (36), (31), (29), (24), (23), (23), (23), (23), (22), (20), (19), (19), (18), (15), (13), (11), (11), (11), (11), (10), (9), (9), (8), (7), (7), (7), (4), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(1206), (446), (365), (257), (248), (236), (200), (185), (182), (39), (35), (19), (16), (13), (11), (11), (9), (7), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN2661843) Images
Cross-Reactivity Chimpanzee, Marmoset
Purification The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Clone HIT8a
Isotype IgG1 kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 antibody (ABIN2661843) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Background CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 antibody (ABIN2661843) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 antibody (ABIN2661843) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD8 antibody (ABIN2661843)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Coppieters, Dotta, Amirian, Campbell, Kay, Atkinson, Roep, von Herrath: "Demonstration of islet-autoreactive CD8 T cells in insulitic lesions from recent onset and long-term type 1 diabetes patients." in: The Journal of experimental medicine, Vol. 209, Issue 1, pp. 51-60, 2012

Suzuki, Kubota, Miyazaki, Ishikawa, Ebisawa, Hirohata, Ogura, Mizusawa, Imai, Miyasaka, Nanki: "Serum level of soluble CX3CL1/fractalkine is elevated in patients with polymyositis and dermatomyositis, which is correlated with disease activity." in: Arthritis research & therapy, Vol. 14, Issue 2, pp. R48, 2012

Awasthi, Lubinski, Shaw, Barrett, Cai, Wang, Betts, Kingsley, Distefano, Balliet, Flynn, Casimiro, Bryan, Friedman et al.: "Immunization with a vaccine combining herpes simplex virus 2 (HSV-2) glycoprotein C (gC) and gD subunits improves the protection of dorsal root ganglia in mice and reduces the frequency of recurrent ..." in: Journal of virology, Vol. 85, Issue 20, pp. 10472-86, 2011

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN2661843) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) antibody (FITC) (ABIN2661843) anti-CD8a Molecule (CD8A) antibody (FITC)