anti-Human CD8 antibody for Intracellular Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Human
597
495
127
92
44
26
19
13
13
13
12
12
9
5
5
5
4
4
4
2
2
1
1
Host
Mouse
763
458
197
1
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to APC-Cy7
150
143
91
55
45
36
36
33
29
27
24
23
23
23
23
22
20
19
19
18
14
13
11
11
10
10
9
7
7
7
5
4
4
4
4
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC), Immunoprecipitation (IP), Western Blotting (WB)
1149
446
364
255
245
233
197
184
173
35
23
19
16
13
11
11
9
7
6
6
6
6
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2660733
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2659916 ICFC PerCP Mouse IgG1 kappa Log in to see RPA-T8 7
1 ABIN2657487 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2661843 ICFC FITC Mouse IgG1 kappa Log in to see HIT8a 3
1 ABIN2658735 FACS ICFC APC Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2659018 ICFC PE-Cy5 Mouse IgG1 kappa Log in to see HIT8a 4
1 ABIN2660734 ICFC IP WB APC-Cy7 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2659917 ICFC PerCP Mouse IgG1 kappa Log in to see RPA-T8 7
1 ABIN2657488 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2661844 ICFC FITC Mouse IgG1 kappa Log in to see HIT8a 3
1 ABIN2658734 FACS ICFC APC Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2659019 ICFC PE-Cy5 Mouse IgG1 kappa Log in to see HIT8a 4

Top referenced anti-CD8 antibody for Intracellular Flow Cytometry

  • Human Monoclonal CD8 Primary Antibody for ICFC, IP - ABIN2660733 (6 Publications): Kmieciak, Gowda, Graham, Godder, Bear, Marincola, Manjili: Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. in Journal of translational medicine 2009 (PubMed)

  • Human Monoclonal CD8 Primary Antibody for ICFC, IP - ABIN2660734 (6 Publications): Mack, Tucker, Sokol, Karrer, Kotzin, Whitington, Miller: Biliary atresia is associated with CD4+ Th1 cell-mediated portal tract inflammation. in Pediatric research 2004 (PubMed)

  • Human Monoclonal CD8 Primary Antibody for ICFC - ABIN2659917 (7 Publications): Hams, Armstrong, Barlow, Saunders, Schwartz, Cooke, Fahy, Crotty, Hirani, Flynn, Voehringer, McKenzie, Donnelly, Fallon: IL-25 and type 2 innate lymphoid cells induce pulmonary fibrosis. in Proceedings of the National Academy of Sciences of the United States of America 2014 (PubMed)

  • Human Monoclonal CD8 Primary Antibody for ICFC - ABIN2659916 (7 Publications): Magidovich, Orr, Fass, Abdu, Yifrach: Intrinsic disorder in the C-terminal domain of the Shaker voltage-activated K+ channel modulates its interaction with scaffold proteins. in Proceedings of the National Academy of Sciences of the United States of America 2007 (PubMed)

  • Human Monoclonal CD8 Primary Antibody for Func, ICC - ABIN2657488 (6 Publications): Rout, Else, Yue, Connole, Exley, Kaur: Paucity of CD4+ natural killer T (NKT) lymphocytes in sooty mangabeys is associated with lack of NKT cell depletion after SIV infection. in PLoS ONE 2010 (PubMed)

  • Human Monoclonal CD8 Primary Antibody for Func, ICC - ABIN2657487 (6 Publications): Thakral, Dobbins, Devine, Kavathas: Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination. in Journal of immunology (Baltimore, Md. : 1950) 2008 (PubMed)

  • Human Monoclonal CD8 Primary Antibody for ICFC - ABIN2661844 (3 Publications): Awasthi, Lubinski, Shaw, Barrett, Cai, Wang, Betts, Kingsley, Distefano, Balliet, Flynn, Casimiro, Bryan, Friedman et al.: Immunization with a vaccine combining herpes simplex virus 2 (HSV-2) glycoprotein C (gC) and gD subunits improves the protection of dorsal root ganglia in mice and reduces the frequency of recurrent ... in Journal of virology 2011 (PubMed)

  • Human Monoclonal CD8 Primary Antibody for ICFC - ABIN2661843 (3 Publications): Coppieters, Dotta, Amirian, Campbell, Kay, Atkinson, Roep, von Herrath: Demonstration of islet-autoreactive CD8 T cells in insulitic lesions from recent onset and long-term type 1 diabetes patients. in The Journal of experimental medicine 2012 (PubMed)

  • Human Monoclonal CD8 Primary Antibody for FACS, ICFC - ABIN2658735 (6 Publications): Yoshino, Ami, Terao, Tashiro, Honda: Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. in Experimental animals / Japanese Association for Laboratory Animal Science 2000 (PubMed)

  • Human Monoclonal CD8 Primary Antibody for ICFC - ABIN2659018 (4 Publications): Suzuki, Kubota, Miyazaki, Ishikawa, Ebisawa, Hirohata, Ogura, Mizusawa, Imai, Miyasaka, Nanki: Serum level of soluble CX3CL1/fractalkine is elevated in patients with polymyositis and dermatomyositis, which is correlated with disease activity. in Arthritis research & therapy 2012 (PubMed)

Similar anti-CD8 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 14 Antibodies
ELISA 145 Antibodies
ELISA (Detection) 1 Antibodies
Flow Cytometry (FACS) 419 Antibodies
Functional Studies (Func) 3 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 122 Antibodies
Immunofluorescence (IF) 151 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(597), (495), (127), (92), (44), (26), (19), (13), (13), (13), (12), (12), (9), (5), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host Mouse
(763), (458), (197), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to APC-Cy7
(150), (143), (91), (55), (45), (36), (36), (33), (29), (27), (24), (23), (23), (23), (23), (22), (20), (19), (19), (18), (14), (13), (11), (11), (10), (10), (9), (7), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC), Immunoprecipitation (IP), Western Blotting (WB)
(1149), (446), (364), (255), (245), (233), (197), (184), (173), (35), (23), (19), (16), (13), (11), (11), (9), (7), (6), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
Supplier Log in to see

Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling ProductDetails: References for anti-CD8 antibody (ABIN2660733) Images
Cross-Reactivity Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Pigtailed Macaque, Sooty mangabey (Cercocebus atys)
Purification The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Clone RPA-T8
Isotype IgG1 kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling ProductDetails: References for anti-CD8 antibody (ABIN2660733) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Background CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling ProductDetails: References for anti-CD8 antibody (ABIN2660733) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details ProductDetails: References for anti-CD8 antibody (ABIN2660733) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

ProductDetails: References for anti-CD8 antibody (ABIN2660733)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Rout, Else, Yue, Connole, Exley, Kaur: "Paucity of CD4+ natural killer T (NKT) lymphocytes in sooty mangabeys is associated with lack of NKT cell depletion after SIV infection." in: PLoS ONE, Vol. 5, Issue 3, pp. e9787, 2010

Kmieciak, Gowda, Graham, Godder, Bear, Marincola, Manjili: "Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function." in: Journal of translational medicine, Vol. 7, pp. 89, 2009

Thakral, Dobbins, Devine, Kavathas: "Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 11, pp. 7431-42, 2008

Magidovich, Orr, Fass, Abdu, Yifrach: "Intrinsic disorder in the C-terminal domain of the Shaker voltage-activated K+ channel modulates its interaction with scaffold proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 32, pp. 13022-7, 2007

Mack, Tucker, Sokol, Karrer, Kotzin, Whitington, Miller: "Biliary atresia is associated with CD4+ Th1 cell-mediated portal tract inflammation." in: Pediatric research, Vol. 56, Issue 1, pp. 79-87, 2004

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling ProductDetails: References for anti-CD8 antibody (ABIN2660733) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (APC-Cy7) (ABIN2660733) anti-CD8a Molecule (CD8A) antibody (APC-Cy7)