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anti-Human CD8 antibody for Immunohistochemistry (Acetone-fixed)

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Human
552
471
127
92
42
26
19
13
13
13
12
11
9
4
2
1
1
Host
Mouse
716
435
187
1
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
141
138
86
51
44
36
36
32
29
27
24
23
23
23
22
22
18
18
18
18
14
12
11
11
10
9
7
7
7
7
5
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
1080
428
337
242
242
227
186
182
173
26
22
20
16
13
11
11
9
7
6
6
6
6
3
2
2
2
2
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN965822
$ 295.63
Plus shipping costs $45.00

Top referenced anti-CD8 antibody for Immunohistochemistry (Acetone-fixed)

Similar anti-CD8 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 14 Antibodies
ELISA 143 Antibodies
ELISA (Detection) 1 Antibodies
Flow Cytometry (FACS) 384 Antibodies
Functional Studies (Func) 3 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 122 Antibodies
Immunofluorescence (IF) 144 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(552), (471), (127), (92), (42), (26), (19), (13), (13), (13), (12), (11), (9), (4), (2), (1), (1)
Host Mouse
(716), (435), (187), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(141), (138), (86), (51), (44), (36), (36), (32), (29), (27), (24), (23), (23), (23), (22), (22), (18), (18), (18), (18), (14), (12), (11), (11), (10), (9), (7), (7), (7), (7), (5), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(1080), (428), (337), (242), (242), (227), (186), (182), (173), (26), (22), (20), (16), (13), (11), (11), (9), (7), (6), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN965822) Images
Specificity This antibody consists of a disufide-linked / hetrodimer with a mW of 30 kD or an / homodimer with a MW of 32 kD. The CD molecule binds to HLA class I molecule during interaction of CD 8+ T cells with antigenpresenting cells or with target cells. CD 8+T cells include most of the cytotoxic T cells.
Characteristics This antibody is directed against alpha chain of CD8 molecule.
Purification Purified.
Clone B441 (143-44 )
Isotype IgG kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN965822) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Molecular Weight 32 kDa
Gene ID 925
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN965822) Images back to top
Application Notes Flow: not tested in our lab.
IHC (acetone-fixed frozen).
Staining Procedure: Acetone-fixed tissue sections at 1:50-1:100 Antibody
Comment

Cellular Localization: Cell membrane.
Recommended Positive Control: Human peripheral lymphocytes, Human tonsil.

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN965822) Images back to top
Format Liquid
Concentration 0.2 mg/0.5 ml.
Buffer Protein A/G purified, 0.2 mg in 50 mM Borate, 150mM NaCl, 20 % Glycerol, pH 8.0
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-CD8 Antibody (ABIN965822)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Akimoto, Kaneko, Sekigawa, Hashimoto, Kaneko, Yamamoto: "Binding of HIV-2 envelope glycoprotein to CD8 molecules and related chemokine production." in: Immunology, Vol. 95, Issue 2, pp. 214-8, 1999

Washizuka, Aizawa, Chinushi, Naitoh, Miyajima, Kusano, Kitazawa, Uchiyama, Takahashi, Shibata: "Alternation of QRS morphology and effect of radiofrequency ablation in idiopathic ventricular tachycardia." in: Pacing and clinical electrophysiology : PACE, Vol. 18, Issue 1 Pt 1, pp. 18-27, 1995

Background publications

Parnes, Seong: "CD4 and CD8 in T cell lineage commitment: alterations induced by expression of a CD8/CD4 chimeric transgene." in: Seminars in immunology, Vol. 6, Issue 4, pp. 221-9, 1995