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anti-Human CD8 antibody for Immunohistochemistry (Acetone-fixed)

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Human
1068
538
168
73
47
42
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
8
5
3
3
2
2
2
2
2
1
1
1
Host
Mouse
1302
519
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
264
219
144
86
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
1638
469
374
351
290
284
264
245
215
37
33
24
23
17
14
10
7
7
6
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN965822
$ 295.63
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN2165022 IHC (af) FACS IP Mouse IgG1 Log in to see
0.0008709465 ABIN2169198 IHC (af) IHC (fp) WB Mouse IgG2b Log in to see
0.0008709465 ABIN2165818 IHC (af) FACS Rat IgG2b Log in to see
0.0008709465 ABIN2165817 IHC (af) FACS Rat IgG2b Log in to see
0.0008709465 ABIN2166818 IHC (af) FACS IP Mouse IgG1 Log in to see
0.0008709465 ABIN2166819 IHC (af) FACS IP Mouse IgG1 Log in to see
0.0008709465 ABIN2166821 IHC (af) IHC (fp) Mouse IgG2b Log in to see
0.0008709465 ABIN2166820 IHC (af) FACS IP Mouse IgG1 Log in to see
0.0008709465 ABIN2166798 IHC (af) FACS Mouse IgG1 Log in to see RPA-T8

Top referenced anti-CD8 antibody for Immunohistochemistry (Acetone-fixed)

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Application / Reactivity Human
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Cytotoxicity Test (CyTox) 7 Antibodies
ELISA 181 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 825 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 167 Antibodies
Immunofluorescence (IF) 188 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 190 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(1068), (538), (168), (73), (47), (42), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (8), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(1302), (519), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(264), (219), (144), (86), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(1638), (469), (374), (351), (290), (284), (264), (245), (215), (37), (33), (24), (23), (17), (14), (10), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN965822) Images
Specificity This antibody consists of a disufide-linked / hetrodimer with a mW of 30 kD or an / homodimer with a MW of 32 kD. The CD molecule binds to HLA class I molecule during interaction of CD 8+ T cells with antigenpresenting cells or with target cells. CD 8+T cells include most of the cytotoxic T cells.
Characteristics This antibody is directed against alpha chain of CD8 molecule.
Purification Purified.
Clone B441 (143-44 )
Isotype IgG kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN965822) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Molecular Weight 32 kDa
Gene ID 925
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN965822) Images back to top
Application Notes Flow: not tested in our lab.
IHC (acetone-fixed frozen).
Staining Procedure: Acetone-fixed tissue sections at 1:50-1:100 Antibody
Comment

Cellular Localization: Cell membrane.
Recommended Positive Control: Human peripheral lymphocytes, Human tonsil.

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN965822) Images back to top
Format Liquid
Concentration 0.2 mg/0.5 ml.
Buffer Protein A/G purified, 0.2 mg in 50 mM Borate, 150mM NaCl, 20 % Glycerol, pH 8.0
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-CD8 Antibody (ABIN965822)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Akimoto, Kaneko, Sekigawa, Hashimoto, Kaneko, Yamamoto: "Binding of HIV-2 envelope glycoprotein to CD8 molecules and related chemokine production." in: Immunology, Vol. 95, Issue 2, pp. 214-8, 1999

Washizuka, Aizawa, Chinushi, Naitoh, Miyajima, Kusano, Kitazawa, Uchiyama, Takahashi, Shibata: "Alternation of QRS morphology and effect of radiofrequency ablation in idiopathic ventricular tachycardia." in: Pacing and clinical electrophysiology : PACE, Vol. 18, Issue 1 Pt 1, pp. 18-27, 1995

Background publications

Parnes, Seong: "CD4 and CD8 in T cell lineage commitment: alterations induced by expression of a CD8/CD4 chimeric transgene." in: Seminars in immunology, Vol. 6, Issue 4, pp. 221-9, 1995