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anti-Human CD8 antibody for Immunohistochemistry (Acetone-fixed)

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Human
1025
538
164
73
47
41
22
22
20
20
19
16
15
13
13
12
12
12
11
11
10
10
8
3
3
2
2
2
2
1
1
1
1
Host
Mouse
1276
510
193
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
258
217
138
81
56
44
41
37
33
29
28
28
26
25
24
24
24
24
20
20
19
17
16
14
12
11
10
10
10
6
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
1617
438
351
337
269
262
240
235
199
39
37
24
23
18
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN965822
$ 295.63
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087055285 ABIN2165022 IHC (af) FACS IP Mouse IgG1 Log in to see
0.00087055285 ABIN2169198 IHC (af) IHC (fp) WB Mouse IgG2b Log in to see
0.00087055285 ABIN2166818 IHC (af) FACS IP Mouse IgG1 Log in to see
0.00087055285 ABIN2166820 IHC (af) FACS IP Mouse IgG1 Log in to see
0.00087055285 ABIN2166819 IHC (af) FACS IP Mouse IgG1 Log in to see
0.00087055285 ABIN2166821 IHC (af) IHC (fp) Mouse IgG2b Log in to see
0.00087055285 ABIN2165818 IHC (af) FACS Rat IgG2b Log in to see
0.00087055285 ABIN2165817 IHC (af) FACS Rat IgG2b Log in to see
0.00087055285 ABIN2166798 IHC (af) FACS Mouse IgG1 Log in to see RPA-T8

Top referenced anti-CD8 antibody for Immunohistochemistry (Acetone-fixed)

Similar anti-CD8 Antibodies

Application / Reactivity Human
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Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
Co-Stimulation (CoSt) 2 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
ELISA 165 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 782 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 149 Antibodies
Immunofluorescence (IF) 167 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 166 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(1025), (538), (164), (73), (47), (41), (22), (22), (20), (20), (19), (16), (15), (13), (13), (12), (12), (12), (11), (11), (10), (10), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1276), (510), (193), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(258), (217), (138), (81), (56), (44), (41), (37), (33), (29), (28), (28), (26), (25), (24), (24), (24), (24), (20), (20), (19), (17), (16), (14), (12), (11), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(1617), (438), (351), (337), (269), (262), (240), (235), (199), (39), (37), (24), (23), (18), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN965822) Images
Specificity This antibody consists of a disufide-linked / hetrodimer with a mW of 30 kD or an / homodimer with a MW of 32 kD. The CD molecule binds to HLA class I molecule during interaction of CD 8+ T cells with antigenpresenting cells or with target cells. CD 8+T cells include most of the cytotoxic T cells.
Characteristics This antibody is directed against alpha chain of CD8 molecule.
Purification Purified.
Clone B441 (143-44 )
Isotype IgG kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN965822) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Molecular Weight 32 kDa
Gene ID 925
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN965822) Images back to top
Application Notes Flow: not tested in our lab.
IHC (acetone-fixed frozen).
Staining Procedure: Acetone-fixed tissue sections at 1:50-1:100 Antibody
Comment

Cellular Localization: Cell membrane.
Recommended Positive Control: Human peripheral lymphocytes, Human tonsil.

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN965822) Images back to top
Format Liquid
Concentration 0.2 mg/0.5 ml.
Buffer Protein A/G purified, 0.2 mg in 50 mM Borate, 150mM NaCl, 20 % Glycerol, pH 8.0
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-CD8 Antibody (ABIN965822)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Akimoto, Kaneko, Sekigawa, Hashimoto, Kaneko, Yamamoto: "Binding of HIV-2 envelope glycoprotein to CD8 molecules and related chemokine production." in: Immunology, Vol. 95, Issue 2, pp. 214-8, 1999

Washizuka, Aizawa, Chinushi, Naitoh, Miyajima, Kusano, Kitazawa, Uchiyama, Takahashi, Shibata: "Alternation of QRS morphology and effect of radiofrequency ablation in idiopathic ventricular tachycardia." in: Pacing and clinical electrophysiology : PACE, Vol. 18, Issue 1 Pt 1, pp. 18-27, 1995

Background publications

Parnes, Seong: "CD4 and CD8 in T cell lineage commitment: alterations induced by expression of a CD8/CD4 chimeric transgene." in: Seminars in immunology, Vol. 6, Issue 4, pp. 221-9, 1995