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anti-Human CD8 antibody for Immunostaining

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Human
1070
538
168
73
47
42
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
8
5
3
3
2
2
2
2
1
1
1
1
Host
Mouse
1304
519
205
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to FITC
262
219
144
87
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunostaining (ISt)
1641
470
373
351
290
285
264
246
215
37
33
25
23
17
14
10
7
7
6
6
6
6
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1002125
$ 408.87
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088641496 ABIN1002126 ISt Biotin Mouse IgG2a Log in to see hCD8
0.00088641496 ABIN1002127 Inhibition ISt Mouse IgG2a Log in to see hCD8
0.00088641496 ABIN284953 FACS ISt IP IHC Mouse IgG1 Log in to see M504511

Similar anti-CD8 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 3 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
Co-Stimulation (CoSt) 2 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
ELISA 181 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 813 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 166 Antibodies
Immunofluorescence (IF) 187 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 186 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(1070), (538), (168), (73), (47), (42), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1304), (519), (205)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to FITC
(262), (219), (144), (87), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunostaining (ISt)
(1641), (470), (373), (351), (290), (285), (264), (246), (215), (37), (33), (25), (23), (17), (14), (10), (7), (7), (6), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling Images
Predicted Reactivity Human CD8 (FITC-labeled)
Purification Ion exchange chromatography
Immunogen Purified human PBL CD8+ T cells
Clone HCD8
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background CD8 belongs to the immunoglobulin superfamily and occurs either as a disulfide-linked homodimer or homomultimer of two 34 kDa subunits (CD8-alpha) or as a heterodimer complexed with another protein named CD8-beta of which there are multiple forms arising by alternative splicing of its mRNA. CD8 functions as an adhesion molecule that binds MHC class 1 molecules. Moreover, it is required for the development of cytotoxic T-cells but not for the development of T-helper cells. CD8(+) cells are cytotoxic T-lymphocytes which are capable of lysing target cells by direct cell contact. Antibodies against CD8 can block the killing activity of cytotoxic cells by inhibiting target cell adhesion. In CD4(+) cells the expression of CD8 can be induced by IL4.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling Images back to top
Application Notes Staining antibody, weakly cytotoxic. For staining, use 10 ml/10⁶ cells. Titer for cytotoxicity should be determined individually by the investigator. Antibody might also be suited for other applications not tested so far.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details Images back to top
Format Lyophilized
Buffer NA
Handling Advice Please Note: Always centrifuge product briefly before opening vial. Reconstitute with 0.5 ml sterile H20 or PBS to give a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Protein concentration: 1 mg/ml (after reconstitution).
Storage 4 °C/-20 °C