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anti-Human CD8 antibody for Western Blotting

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Epitope
AA 150-180, C-Term
103
31
20
19
16
16
14
9
8
7
6
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1056
551
165
77
50
41
22
22
20
20
19
16
15
13
13
12
12
12
11
11
10
10
8
3
3
2
2
2
1
1
1
1
Host
Rabbit
1288
523
218
1
Clonality
Polyclonal
Conjugate
This CD8 antibody is un-conjugated
264
220
141
81
58
46
43
39
35
31
29
28
27
26
26
26
26
25
21
21
19
17
16
15
11
10
10
10
9
7
6
6
4
4
4
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1651
466
447
319
271
268
266
246
229
39
38
25
25
23
18
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN388172
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.022232 ABIN388171 IHC (p) WB Rabbit Ig AA 59-88, N-Term Log in to see Polyclonal 2
3.022232 ABIN1385272 FACS IF (p) IHC (p) WB Rabbit IgG AA 60-110 Log in to see Polyclonal 1
3.022232 ABIN2787942 WB Rabbit Middle Region Log in to see Polyclonal
3.022232 ABIN2774246 WB Rabbit Middle Region Log in to see Polyclonal
3.022232 ABIN3043499 IHC (p) WB Rabbit IgG AA 22-187 Log in to see Polyclonal
3.022232 ABIN2705816 WB Rabbit Center Log in to see Polyclonal
3.022232 ABIN1077936 ICC IHC IP WB Rabbit IgG AA 22-198 Log in to see Polyclonal
3.022232 ABIN3030431 FACS IHC ELISA WB Rabbit Ig Fraction AA 150-180 Log in to see Polyclonal
3.022232 ABIN3028555 IHC ELISA WB Rabbit Ig Fraction AA 59-88 Log in to see Polyclonal
3.022232 ABIN560229 ELISA WB Mouse AA 22-131, partial Log in to see Polyclonal
3.022232 ABIN929133 WB Rabbit Middle Region Log in to see Polyclonal
3.022232 ABIN929134 WB Rabbit Middle Region Log in to see Polyclonal
3.022232 ABIN2158143 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
3.022232 ABIN2158142 IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
3.022232 ABIN3030432 IHC (p) WB Rabbit IgG AA 22-187 Log in to see Polyclonal
3.022232 ABIN514145 WB Mouse AA 1-235, full length Log in to see Polyclonal
3.022232 ABIN514146 WB Rabbit AA 1-235, full length Log in to see Polyclonal
1 ABIN4620511 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 2
1 ABIN4620512 EIA IHC (p) WB Rabbit Ig AA 66-96, N-Term Log in to see Polyclonal 2
1 ABIN2661147 FACS WB Biotin Mouse IgG1 kappa Log in to see RPA-T8 6

Top referenced anti-CD8 antibody for Western Blotting

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Application / Reactivity Human
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Blocking Reagent (BR) 3 Antibodies
Co-Stimulation (CoSt) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 17 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
ELISA 199 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 803 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 10 Antibodies
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Immunofluorescence (IF) 180 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 222 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Epitope AA 150-180, C-Term
(103), (31), (20), (19), (16), (16), (14), (9), (8), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1056), (551), (165), (77), (50), (41), (22), (22), (20), (20), (19), (16), (15), (13), (13), (12), (12), (12), (11), (11), (10), (10), (8), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(1288), (523), (218), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(264), (220), (141), (81), (58), (46), (43), (39), (35), (31), (29), (28), (27), (26), (26), (26), (26), (25), (21), (21), (19), (17), (16), (15), (11), (10), (10), (10), (9), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1651), (466), (447), (319), (271), (268), (266), (246), (229), (39), (38), (25), (25), (23), (18), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN388172) Images
Specificity This CD8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158~188 amino acids from the C-terminal region of human CD8A.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This CD8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-180 AA from the C-terminal region of human CD8A.
Clone RB13642
Isotype Ig

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN388172) Images back to top
Antigen
Alternative Name CD8A (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a co-receptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional co-receptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a antigen, MAL
Molecular Weight 25729 DA
Gene ID 925
UniProt P01732
Research Area Stem Cells, Cell Structure
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN388172) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN388172) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CD8 Antibody (ABIN388172)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Mancebo, Moreno-Pelayo, Mencía, de la Calle-Martín, Allende, Sivadorai, Kalaydjieva, Bertranpetit, Coto, Calleja-Antolín, Ruiz-Contreras, Paz-Artal: "Gly111Ser mutation in CD8A gene causing CD8 immunodeficiency is found in Spanish Gypsies." in: Molecular immunology, Vol. 45, Issue 2, pp. 479-84, 2007

Zhou, Wang, Zhu, You, Zou: "CD8+FOXP3+T cells from renal transplant recipients in quiescence induce immunoglobulin-like transcripts-3 and -4 on dendritic cells from their respective donors." in: Transplantation proceedings, Vol. 39, Issue 10, pp. 3065-7, 2007

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN388172) back to top
Supplier Images
Western Blotting (WB) image for anti-CD8 antibody (CD8a Molecule) (AA 150-180) (ABIN388172) Western blot analysis of CD8A(arrow) using rabbit polyclonal CD8A Antibody (C-term) (...
Immunohistochemistry (IHC) image for anti-CD8 antibody (CD8a Molecule) (AA 150-180) (ABIN388172) Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD8A an...
Immunohistochemistry (IHC) image for anti-CD8 antibody (CD8a Molecule) (AA 150-180) (ABIN388172) Flow cytometric analysis of NCI-H292 cells using CD8A Antibody (C-term)(bottom histog...