anti-Human CD8 antibody for Western Blotting

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Epitope
Middle Region
85
40
19
15
14
14
13
12
11
9
9
9
7
6
5
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human
617
525
127
92
44
26
22
19
13
13
13
11
9
5
5
5
4
4
4
2
2
1
1
Host
Rabbit
791
488
197
2
1
Clonality
Polyclonal
Conjugate
This CD8 antibody is un-conjugated
151
149
93
60
48
41
40
36
31
29
24
23
23
23
23
22
20
19
19
18
15
13
11
11
11
11
10
9
9
8
7
7
7
4
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
1206
446
365
257
248
235
200
185
182
39
35
20
16
13
11
11
9
7
6
6
6
3
2
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2787942
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN2705816 WB Rabbit Center Log in to see Polyclonal
3.2182703 ABIN3043499 IHC (p) WB Rabbit IgG AA 22-187 Log in to see Polyclonal
3.2182703 ABIN388172 FACS IHC (p) WB Rabbit Ig AA 150-180, C-Term Log in to see Polyclonal 2
3.2182703 ABIN388171 IHC (p) WB Rabbit Ig AA 59-88, N-Term Log in to see Polyclonal 2
3.2182703 ABIN2774246 WB Rabbit Middle Region Log in to see Polyclonal
3.2182703 ABIN1077936 ICC IHC IP WB Rabbit IgG AA 22-198 Log in to see Polyclonal
3.2182703 ABIN2681738 ELISA WB Rabbit IgG AA 1-182 Log in to see Polyclonal
3.2182703 ABIN3028555 IHC ELISA WB Rabbit Ig Fraction AA 59-88 Log in to see Polyclonal
3.2182703 ABIN560229 ELISA WB Mouse AA 22-131, partial Log in to see Polyclonal
3.2182703 ABIN5535216 IHC (p) WB Rabbit Ig Fraction AA 59-88, N-Term Log in to see Polyclonal
3.2182703 ABIN929133 WB Rabbit Middle Region Log in to see Polyclonal
3.2182703 ABIN929134 WB Rabbit Middle Region Log in to see Polyclonal
3.2182703 ABIN514146 WB Rabbit AA 1-235, full length Log in to see Polyclonal
3.2182703 ABIN514145 WB Mouse AA 1-235, full length Log in to see Polyclonal
1 ABIN2661147 FACS WB Biotin Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2660733 ICFC IP WB APC-Cy7 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2657487 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2659562 FACS IHC ELISA WB PE-Cy7 Mouse IgG1 kappa Log in to see HIT8a 3
1 ABIN1491225 IHC (p) WB Mouse IgG1 AA 22-182 Log in to see 3H6
1 ABIN2664858 IF WB Mouse IgG1 kappa Log in to see RPA-T8 6

General

Antigen CD8a Molecule (CD8A) Antibodies
Epitope Middle Region
(85), (40), (19), (15), (14), (14), (13), (12), (11), (9), (9), (9), (7), (6), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human
(617), (525), (127), (92), (44), (26), (22), (19), (13), (13), (13), (11), (9), (5), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host Rabbit
(791), (488), (197), (2), (1)
Clonality
Polyclonal
Conjugate This CD8 antibody is un-conjugated
(151), (149), (93), (60), (48), (41), (40), (36), (31), (29), (24), (23), (23), (23), (23), (22), (20), (19), (19), (18), (15), (13), (11), (11), (11), (11), (10), (9), (9), (8), (7), (7), (7), (4), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(1206), (446), (365), (257), (248), (235), (200), (185), (182), (39), (35), (20), (16), (13), (11), (11), (9), (7), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN974701
Sequence GKRLGDTFVL TLSDFRRENE GYYFCSALSN SIMYFSHFVP VFLPAKPTTT
Predicted Reactivity Dog: 79%, Human: 100%
Characteristics This is a rabbit polyclonal antibody against CD8A. It was validated on Western Blot.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CD8A

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD8A (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: CD8, Leu2, MAL, p32
Protein Interaction Partner: CACYBP, UBC, CDK1, LNX2, LNX1, SREK1, LAT, SRSF1, CD8A, CD3D, CD8B, LCK, PTPRC, LGALS1, HLA-E, HLA-G, HLA-C, HLA-B, HLA-A, B2M, CD59,
Protein Size: 235
Molecular Weight 24 kDa
Gene ID 925
NCBI Accession NM_001145873, NP_001139345
UniProt P01732
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 235 AA

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD8a Molecule (CD8A) (Middle Region) antibody (ABIN2787942) WB Suggested Anti-CD8A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Western Blotting (WB) image for anti-CD8a Molecule (CD8A) (Middle Region) antibody (ABIN2787942) WB Suggested Anti-CD8A
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:1562500 ...