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anti-Human CD8 antibody for Western Blotting

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Epitope
AA 150-180, C-Term
106
27
19
16
14
14
8
7
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
1070
538
168
73
47
42
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
8
5
3
3
2
2
2
2
1
1
1
1
Host
Rabbit
1305
519
204
Clonality
Polyclonal
Conjugate
This CD8 antibody is un-conjugated
263
219
144
87
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1640
470
373
351
289
285
263
246
215
37
33
25
23
17
14
10
7
7
6
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN388172
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12917753 ABIN388171 IHC (p) WB Rabbit Ig AA 59-88, N-Term Log in to see Polyclonal 2
0.12917753 ABIN1385272 IF (p) IHC (p) WB Rabbit IgG AA 60-110 Log in to see Polyclonal 1
0.12917753 ABIN2787942 WB Rabbit Middle Region Log in to see Polyclonal
0.12917753 ABIN2774246 WB Rabbit Middle Region Log in to see Polyclonal
0.12917753 ABIN2705816 WB Rabbit Center Log in to see Polyclonal
0.12917753 ABIN3043499 IHC (p) WB Rabbit IgG AA 22-187 Log in to see Polyclonal
0.12917753 ABIN1077936 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-198 Log in to see Polyclonal
0.12917753 ABIN560229 ELISA WB Mouse AA 22-131, partial Log in to see Polyclonal
0.12917753 ABIN2158143 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.12917753 ABIN2158142 IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.12917753 ABIN929134 WB Rabbit Middle Region Log in to see Polyclonal
0.12917753 ABIN929133 WB Rabbit Middle Region Log in to see Polyclonal
0.12917753 ABIN514145 WB Mouse AA 1-235, full length Log in to see Polyclonal
0.12917753 ABIN514146 WB Rabbit AA 1-235, full length Log in to see Polyclonal
0.0011819942 ABIN4620511 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 2
0.0011819942 ABIN4620512 EIA IHC (p) WB Rabbit Ig AA 66-96, N-Term Log in to see Polyclonal 2
0.0011819942 ABIN2661147 FACS WB Biotin Mouse IgG1 kappa Log in to see RPA-T8 6
0.0011819942 ABIN2657487 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
0.0011819942 ABIN2659562 FACS IHC ELISA WB PE-Cy7 Mouse IgG1 kappa Log in to see HIT8a 3
0.0011819942 ABIN2660733 ICFC IP WB APC-Cy7 Mouse IgG1 kappa Log in to see RPA-T8 6

Top referenced anti-CD8 antibody for Western Blotting

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Application / Reactivity Human
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Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
Co-Stimulation (CoSt) 2 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
ELISA 181 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 813 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 166 Antibodies
Immunofluorescence (IF) 187 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 186 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Epitope AA 150-180, C-Term
(106), (27), (19), (16), (14), (14), (8), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1070), (538), (168), (73), (47), (42), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(1305), (519), (204)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(263), (219), (144), (87), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1640), (470), (373), (351), (289), (285), (263), (246), (215), (37), (33), (25), (23), (17), (14), (10), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN388172) Images
Specificity This CD8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158~188 amino acids from the C-terminal region of human CD8A.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This CD8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-180 AA from the C-terminal region of human CD8A.
Clone RB13642
Isotype Ig

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN388172) Images back to top
Antigen
Alternative Name CD8A (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a co-receptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional co-receptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a antigen, MAL
Molecular Weight 25729 DA
Gene ID 925
UniProt P01732
Research Area Stem Cells, Cell Structure
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN388172) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN388172) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CD8 Antibody (ABIN388172)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Mancebo, Moreno-Pelayo, Mencía, de la Calle-Martín, Allende, Sivadorai, Kalaydjieva, Bertranpetit, Coto, Calleja-Antolín, Ruiz-Contreras, Paz-Artal: "Gly111Ser mutation in CD8A gene causing CD8 immunodeficiency is found in Spanish Gypsies." in: Molecular immunology, Vol. 45, Issue 2, pp. 479-84, 2007

Zhou, Wang, Zhu, You, Zou: "CD8+FOXP3+T cells from renal transplant recipients in quiescence induce immunoglobulin-like transcripts-3 and -4 on dendritic cells from their respective donors." in: Transplantation proceedings, Vol. 39, Issue 10, pp. 3065-7, 2007

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN388172) back to top
Supplier Images
Western Blotting (WB) image for anti-CD8 antibody (CD8a Molecule) (AA 150-180) (ABIN388172) Western blot analysis of CD8A(arrow) using rabbit polyclonal CD8A Antibody (C-term) (...
Immunohistochemistry (IHC) image for anti-CD8 antibody (CD8a Molecule) (AA 150-180) (ABIN388172) Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD8A an...
Immunohistochemistry (IHC) image for anti-CD8 antibody (CD8a Molecule) (AA 150-180) (ABIN388172) Flow cytometric analysis of NCI-H292 cells using CD8A Antibody (C-term)(bottom histog...