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anti-Human CD8 antibody for Western Blotting

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Epitope
AA 150-180, C-Term
83
23
19
15
14
14
13
12
10
9
9
9
7
5
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
552
471
127
92
42
26
19
13
13
13
12
11
9
4
2
1
1
Host
Rabbit
717
435
186
1
1
Clonality
Polyclonal
Conjugate
This CD8 antibody is un-conjugated
141
138
86
51
44
36
36
32
29
27
24
23
23
23
22
22
18
18
18
18
14
12
11
11
10
9
7
7
7
7
5
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1080
428
338
242
241
226
186
182
173
26
22
20
16
13
11
11
9
7
6
6
6
6
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN388172
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0726016 ABIN388171 IHC (p) WB Rabbit Ig AA 59-88, N-Term Log in to see Polyclonal 2
3.0726016 ABIN2774246 WB Rabbit Middle Region Log in to see Polyclonal
3.0726016 ABIN2787942 WB Rabbit Middle Region Log in to see Polyclonal
3.0726016 ABIN3043499 IHC (p) WB Rabbit IgG AA 22-187 Log in to see Polyclonal
3.0726016 ABIN2705816 WB Rabbit Center Log in to see Polyclonal
3.0726016 ABIN1077936 ICC IHC IP WB Rabbit IgG AA 22-198 Log in to see Polyclonal
3.0726016 ABIN3028555 IHC ELISA WB Rabbit Ig Fraction AA 59-88 Log in to see Polyclonal
3.0726016 ABIN560229 ELISA WB Mouse AA 22-131, partial Log in to see Polyclonal
3.0726016 ABIN929133 WB Rabbit Middle Region Log in to see Polyclonal
3.0726016 ABIN929134 WB Rabbit Middle Region Log in to see Polyclonal
3.0726016 ABIN514145 WB Mouse AA 1-235, full length Log in to see Polyclonal
3.0726016 ABIN514146 WB Rabbit AA 1-235, full length Log in to see Polyclonal
1 ABIN2661147 FACS WB Biotin Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2657487 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2659562 FACS IHC ELISA WB PE-Cy7 Mouse IgG1 kappa Log in to see HIT8a 3
1 ABIN2660733 ICFC IP WB APC-Cy7 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN1491225 IHC (p) WB Mouse IgG1 AA 22-182 Log in to see 3H6
1 ABIN2664858 IF WB Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN575655 WB Rabbit AA 91-140 Log in to see Polyclonal
1 ABIN575769 WB Rabbit AA 61-110 Log in to see Polyclonal

Top referenced anti-CD8 antibody for Western Blotting

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Application / Reactivity Human
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Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 14 Antibodies
ELISA 143 Antibodies
ELISA (Detection) 1 Antibodies
Flow Cytometry (FACS) 384 Antibodies
Functional Studies (Func) 3 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 122 Antibodies
Immunofluorescence (IF) 144 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Epitope AA 150-180, C-Term
(83), (23), (19), (15), (14), (14), (13), (12), (10), (9), (9), (9), (7), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(552), (471), (127), (92), (42), (26), (19), (13), (13), (13), (12), (11), (9), (4), (2), (1), (1)
Host Rabbit
(717), (435), (186), (1), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(141), (138), (86), (51), (44), (36), (36), (32), (29), (27), (24), (23), (23), (23), (22), (22), (18), (18), (18), (18), (14), (12), (11), (11), (10), (9), (7), (7), (7), (7), (5), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1080), (428), (338), (242), (241), (226), (186), (182), (173), (26), (22), (20), (16), (13), (11), (11), (9), (7), (6), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN388172) Images
Specificity This CD8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158~188 amino acids from the C-terminal region of human CD8A.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This CD8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-180 AA from the C-terminal region of human CD8A.
Clone RB13642
Isotype Ig

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN388172) Images back to top
Antigen
Alternative Name CD8A (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a co-receptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional co-receptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a antigen, MAL
Molecular Weight 25729 DA
Gene ID 925
UniProt P01732
Research Area Stem Cells, Cell Structure
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN388172) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN388172) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CD8 Antibody (ABIN388172)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Mancebo, Moreno-Pelayo, Mencía, de la Calle-Martín, Allende, Sivadorai, Kalaydjieva, Bertranpetit, Coto, Calleja-Antolín, Ruiz-Contreras, Paz-Artal: "Gly111Ser mutation in CD8A gene causing CD8 immunodeficiency is found in Spanish Gypsies." in: Molecular immunology, Vol. 45, Issue 2, pp. 479-84, 2007

Zhou, Wang, Zhu, You, Zou: "CD8+FOXP3+T cells from renal transplant recipients in quiescence induce immunoglobulin-like transcripts-3 and -4 on dendritic cells from their respective donors." in: Transplantation proceedings, Vol. 39, Issue 10, pp. 3065-7, 2007

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN388172) back to top
Supplier Images
Western Blotting (WB) image for anti-CD8 antibody (CD8a Molecule) (AA 150-180) (ABIN388172) Western blot analysis of CD8A(arrow) using rabbit polyclonal CD8A Antibody (C-term) (...
Immunohistochemistry (IHC) image for anti-CD8 antibody (CD8a Molecule) (AA 150-180) (ABIN388172) Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD8A an...
Immunohistochemistry (IHC) image for anti-CD8 antibody (CD8a Molecule) (AA 150-180) (ABIN388172) Flow cytometric analysis of NCI-H292 cells using CD8A Antibody (C-term)(bottom histog...