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anti-Human CD8 antibody for Western Blotting

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Epitope
AA 59-88, N-Term
104
29
20
19
16
15
14
8
7
6
5
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
1025
538
164
73
47
41
22
22
20
20
19
16
15
13
13
12
12
12
11
11
10
10
8
3
3
2
2
2
2
1
1
1
1
Host
Rabbit
1277
510
192
1
Clonality
Polyclonal
Conjugate
This CD8 antibody is un-conjugated
258
217
138
81
56
44
41
37
33
29
28
28
26
25
24
24
24
24
20
20
19
17
16
14
12
11
10
10
10
6
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1618
439
351
337
268
262
239
235
199
39
37
25
23
18
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN388171
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.124960825 ABIN388172 FACS IHC (p) WB Rabbit Ig AA 150-180, C-Term Log in to see Polyclonal 2
0.124960825 ABIN1385272 FACS IF (p) IHC (p) WB Rabbit IgG AA 60-110 Log in to see Polyclonal 1
0.124960825 ABIN2774246 WB Rabbit Middle Region Log in to see Polyclonal
0.124960825 ABIN2787942 WB Rabbit Middle Region Log in to see Polyclonal
0.124960825 ABIN3043499 IHC (p) WB Rabbit IgG AA 22-187 Log in to see Polyclonal
0.124960825 ABIN2705816 WB Rabbit Center Log in to see Polyclonal
0.124960825 ABIN1077936 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-198 Log in to see Polyclonal
0.124960825 ABIN560229 ELISA WB Mouse AA 22-131, partial Log in to see Polyclonal
0.124960825 ABIN929133 WB Rabbit Middle Region Log in to see Polyclonal
0.124960825 ABIN929134 WB Rabbit Middle Region Log in to see Polyclonal
0.124960825 ABIN2158142 IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.124960825 ABIN2158143 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.124960825 ABIN514146 WB Rabbit AA 1-235, full length Log in to see Polyclonal
0.124960825 ABIN514145 WB Mouse AA 1-235, full length Log in to see Polyclonal
0.124960825 ABIN3030431 FACS IHC ELISA WB Rabbit Ig Fraction AA 150-180 Log in to see Polyclonal
0.124960825 ABIN3028555 IHC ELISA WB Rabbit Ig Fraction AA 59-88 Log in to see Polyclonal
0.124960825 ABIN3030432 IHC (p) WB Rabbit IgG AA 22-187 Log in to see Polyclonal
0.0012207722 ABIN4620512 EIA IHC (p) WB Rabbit Ig AA 66-96, N-Term Log in to see Polyclonal 2
0.0012207722 ABIN4620511 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 2
0.0012207722 ABIN2661147 FACS WB Biotin Mouse IgG1 kappa Log in to see RPA-T8 6

Top referenced anti-CD8 antibody for Western Blotting

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Application / Reactivity Human
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Blocking Reagent (BR) 3 Antibodies
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ELISA 165 Antibodies
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Enzyme Immunoassay (EIA) 4 Antibodies
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Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 149 Antibodies
Immunofluorescence (IF) 167 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 166 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Epitope AA 59-88, N-Term
(104), (29), (20), (19), (16), (15), (14), (8), (7), (6), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1025), (538), (164), (73), (47), (41), (22), (22), (20), (20), (19), (16), (15), (13), (13), (12), (12), (12), (11), (11), (10), (10), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(1277), (510), (192), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(258), (217), (138), (81), (56), (44), (41), (37), (33), (29), (28), (28), (26), (25), (24), (24), (24), (24), (20), (20), (19), (17), (16), (14), (12), (11), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1618), (439), (351), (337), (268), (262), (239), (235), (199), (39), (37), (25), (23), (18), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN388171) Images
Specificity This CD8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66~96 amino acids from the N-terminal region of human CD8A.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This CD8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 AA from the N-terminal region of human CD8A.
Clone RB13990
Isotype Ig

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN388171) Images back to top
Antigen
Alternative Name CD8A (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a antigen, MAL
Molecular Weight 25729 DA
Gene ID 925
UniProt P01732
Research Area Stem Cells, Cell Structure
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN388171) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN388171) Images back to top
Format Liquid
Concentration 0.38 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CD8 Antibody (ABIN388171)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Mancebo, Moreno-Pelayo, Mencía, de la Calle-Martín, Allende, Sivadorai, Kalaydjieva, Bertranpetit, Coto, Calleja-Antolín, Ruiz-Contreras, Paz-Artal: "Gly111Ser mutation in CD8A gene causing CD8 immunodeficiency is found in Spanish Gypsies." in: Molecular immunology, Vol. 45, Issue 2, pp. 479-84, 2007

Zhou, Wang, Zhu, You, Zou: "CD8+FOXP3+T cells from renal transplant recipients in quiescence induce immunoglobulin-like transcripts-3 and -4 on dendritic cells from their respective donors." in: Transplantation proceedings, Vol. 39, Issue 10, pp. 3065-7, 2007

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN388171) back to top
Supplier Images
Western Blotting (WB) image for anti-CD8 antibody (CD8a Molecule) (AA 59-88) (ABIN388171) Western blot analysis of anti-CD8A Antibody (N-term) (ABIN388171) in Hela cell line l...
Western Blotting (WB) image for anti-CD8 antibody (CD8a Molecule) (AA 59-88) (ABIN388171) Western blot analysis of CD8A(arrow) using rabbit polyclonal CD8A Antibody (N-term) (...
Immunohistochemistry (IHC) image for anti-CD8 antibody (CD8a Molecule) (AA 59-88) (ABIN388171) Formalin-fixed and paraffin-embedded human tonsil tissue reacted with CD8A Antibody (...