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anti-Monkey CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Human, Monkey
1050
536
161
77
50
41
22
22
20
20
19
16
15
13
13
12
12
12
11
11
10
10
7
3
3
2
2
2
2
1
1
1
1
Host
Mouse
1283
510
215
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to APC
261
219
141
80
57
45
42
38
34
30
29
28
26
26
25
25
25
24
21
21
19
17
16
15
11
10
10
10
9
6
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1626
452
449
316
271
267
261
246
234
39
33
25
24
23
18
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN320010
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN320012 FACS FITC Mouse IgG1 Log in to see LT8 5
1 ABIN320014 FACS PE Mouse IgG1 Log in to see LT8 1
1 ABIN317156 FACS IHC (fro) Rat IgG2b Log in to see YTC182-20 1
1 ABIN120106 FACS IHC (fro) Rat IgG2b Log in to see YTC141-1HL
1 ABIN320011 FACS Biotin Mouse IgG1 Log in to see LT8
1 ABIN320013 FACS IHC (fro) IP Mouse IgG1 Log in to see LT8

Top referenced anti-CD8 antibody for Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Human Monkey
Cytometry by Time of Flight (CyTOF) 17 Antibodies
Western Blotting (WB) 210 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Multiplex Assay (MA) 1 Antibodies
Microcytotoxicity Assay (MCA) 1 Antibodies
Mass Cytometry (CyTOF) 6 Antibodies
Intracellular Flow Cytometry (ICFC) 15 Antibodies
In vitro Assay (in vitro) 2 Antibodies
Immunostaining (ISt) 4 Antibodies
Immunoprecipitation (IP) 144 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 158 Antibodies
Immunohistochemistry (IHC) 221 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 235 Antibodies 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human, Monkey
(1050), (536), (161), (77), (50), (41), (22), (22), (20), (20), (19), (16), (15), (13), (13), (12), (12), (12), (11), (11), (10), (10), (7), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1283), (510), (215), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to APC
(261), (219), (141), (80), (57), (45), (42), (38), (34), (30), (29), (28), (26), (26), (25), (25), (25), (24), (21), (21), (19), (17), (16), (15), (11), (10), (10), (10), (9), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1626), (452), (449), (316), (271), (267), (261), (246), (234), (39), (33), (25), (24), (23), (18), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN320010) Images
Specificity This antibody recognises the alpha-chain of the CD8 antigen, a 32 kDa glycoprotein expressed by the cytotoxicsuppressor subset of T cells and weakly by NK cells.
Purification Ion Exchange Chromatography
Immunogen Normal human peripheral blood lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Clone LT8
Isotype IgG1

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN320010) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 925
NCBI Accession NP_001759
UniProt P01732
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN320010) Images back to top
Application Notes Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells or 100 μL human wholeblood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN320010) Images back to top
Reconstitution Restore with 1 mL distilled water
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to and following reconstitution store the antibody at -20 °C.

References for anti-CD8 Antibody (ABIN320010)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Parnes: "Molecular biology and function of CD4 and CD8." in: Advances in immunology, Vol. 44, pp. 265-311, 1989