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anti-Monkey CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Human, Monkey
1070
538
168
73
47
42
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
7
5
3
3
2
2
2
2
1
1
1
1
Host
Mouse
1304
519
205
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to FITC
262
219
144
87
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1640
470
373
351
290
285
264
246
215
37
33
25
23
17
14
10
7
7
6
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN320012
$ 198.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010452945 ABIN320014 FACS PE Mouse IgG1 Log in to see LT8 1
0.0010452945 ABIN320010 FACS APC Mouse IgG1 Log in to see LT8 1
0.0010452945 ABIN317156 FACS IHC (fro) Rat IgG2b Log in to see YTC182-20 1
0.0010452945 ABIN120106 FACS IHC (fro) Rat IgG2b Log in to see YTC141-1HL
0.0010452945 ABIN320011 FACS Biotin Mouse IgG1 Log in to see LT8
0.0010452945 ABIN320013 FACS IHC (fro) IP Mouse IgG1 Log in to see LT8

Top referenced anti-CD8 antibody for Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Human Monkey
Western Blotting (WB) 232 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Multiplex Assay (MA) 1 Antibodies
Microcytotoxicity Assay (MCA) 1 Antibodies
Mass Cytometry (CyTOF) 6 Antibodies
Light Microscopy (LM) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 15 Antibodies
Immunostaining (ISt) 4 Antibodies
Immunoprecipitation (IP) 178 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 167 Antibodies
Immunohistochemistry (IHC) 186 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 256 Antibodies 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human, Monkey
(1070), (538), (168), (73), (47), (42), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (7), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1304), (519), (205)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to FITC
(262), (219), (144), (87), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1640), (470), (373), (351), (290), (285), (264), (246), (215), (37), (33), (25), (23), (17), (14), (10), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN320012) Images
Specificity This antibody recognises the CD8 cell surface glycoprotein.
Purification Ion Exchange Chromatography.
Immunogen Normal Human peripheral blood lymphocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Clone LT8
Isotype IgG1

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN320012) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background CD8 is expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells. The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 925
NCBI Accession NP_001759
UniProt P01732
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN320012) Images back to top
Application Notes Flow cytometry: Neat - 1/5, use 10 μL of the suggested working dilution to label 10^6 cells orcells or 100l human whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN320012) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD8 Antibody (ABIN320012)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Nelson, Lever, Dean, Savage, Salguero, Pearce, Stevens, Simpson: "Characterization of lethal inhalational infection with Francisella tularensis in the common marmoset (Callithrix jacchus)." in: Journal of medical microbiology, Vol. 59, Issue Pt 9, pp. 1107-13, 2010

Kap, van Meurs, van Driel, Koopman, Melief, Brok, Laman, t Hart: "A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 57, Issue 12, pp. 1159-67, 2009

Gibbings, Marcet-Palacios, Sekar, Ng, Befus: "CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses." in: BMC immunology, Vol. 8, pp. 12, 2007

Zarkesh-Esfahani, Pockley, Metcalfe, Bidlingmaier, Wu, Ajami, Weetman, Strasburger, Ross: "High-dose leptin activates human leukocytes via receptor expression on monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4593-9, 2001

Parnes: "Molecular biology and function of CD4 and CD8." in: Advances in immunology, Vol. 44, pp. 265-311, 1989