You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) CD8 antibody for Intracellular Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Mouse (Murine)
766
403
130
68
28
27
25
20
20
16
15
12
12
11
11
10
10
9
8
8
3
3
2
2
2
2
1
1
1
Host
Rat
961
390
161
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to PE-Cy5
243
190
122
86
30
29
27
18
18
17
16
15
13
11
10
10
10
9
8
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC)
1178
282
232
188
136
111
105
81
69
36
33
25
22
17
14
10
7
6
6
6
5
4
4
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2659022
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008704084 ABIN2661849 ICFC FITC Rat IgG2b lambda Log in to see 5H10-1 6
0.0008704084 ABIN2656838 ICFC Alexa Fluor 594 Rat IgG2a kappa Log in to see 53-6-7 10
0.0008704084 ABIN2662053 ICFC FITC Rat IgG2a kappa Log in to see 53-6-7 10
0.0008704084 ABIN2659023 ICFC PE-Cy5 Rat IgG2a kappa Log in to see 53-6-7 11
0.0008704084 ABIN2661848 ICFC FITC Rat IgG2b lambda Log in to see 5H10-1 6
0.0008704084 ABIN2662052 ICFC FITC Rat IgG2a kappa Log in to see 53-6-7 15

Top referenced anti-CD8 antibody for Intracellular Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytotoxicity Test (CyTox) 9 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 345 Antibodies
Functional Studies (Func) 23 Antibodies
Immunocytochemistry (ICC) 27 Antibodies
Immunofluorescence (IF) 40 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 72 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 52 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 62 Antibodies
Intracellular Flow Cytometry (ICFC) 7 Antibodies
Separation (Sep) 2 Antibodies
Western Blotting (WB) 20 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(766), (403), (130), (68), (28), (27), (25), (20), (20), (16), (15), (12), (12), (11), (11), (10), (10), (9), (8), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rat
(961), (390), (161)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to PE-Cy5
(243), (190), (122), (86), (30), (29), (27), (18), (18), (17), (16), (15), (13), (11), (10), (10), (10), (9), (8), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(1178), (282), (232), (188), (136), (111), (105), (81), (69), (36), (33), (25), (22), (17), (14), (10), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 10 references available
Supplier Log in to see

Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2659022) Images
Purification The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Clone 53-6-7
Isotype IgG2a kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN2659022) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Background CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN2659022) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN2659022) Images back to top
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD8 Antibody (ABIN2659022)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Bouwer, Alberti-Segui, Montfort, Berkowitz, Higgins: "Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 13, pp. 5102-7, 2006

Kamimura, Sawa, Sato, Agung, Hirano, Murakami: "IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 306-14, 2006

Shih, Racz, Allen: "Differential MHC class II presentation of a pathogenic autoantigen during health and disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3438-48, 2006

Hata, Sakaguchi, Yoshitomi, Iwakura, Sekikawa, Azuma, Kanai, Moriizumi, Nomura, Nakamura, Sakaguchi: "Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice." in: The Journal of clinical investigation, Vol. 114, Issue 4, pp. 582-8, 2004

Fan, Kouda, Nakamura, Takeuchi: "Effects of dietary restriction on spontaneous dermatitis in NC/Nga mice." in: Experimental biology and medicine (Maywood, N.J.), Vol. 226, Issue 11, pp. 1045-50, 2001

Ellmeier, Sawada, Littman: "The regulation of CD4 and CD8 coreceptor gene expression during T cell development." in: Annual review of immunology, Vol. 17, pp. 523-54, 1999

Zamoyska: "The CD8 coreceptor revisited: one chain good, two chains better." in: Immunity, Vol. 1, Issue 4, pp. 243-6, 1995

Takahashi, Nakata, Tanaka, Adachi, Nakauchi, Yagita, Okumura: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992

Ledbetter, Seaman, Tsu, Herzenberg: "Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity." in: The Journal of experimental medicine, Vol. 153, Issue 6, pp. 1503-16, 1981

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2659022) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (PE-Cy5) (ABIN2659022) anti-CD8a Molecule (CD8A) antibody (PE-Cy5)