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anti-Pig (Porcine) CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Pig (Porcine)
1069
538
168
73
47
41
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
8
5
3
3
2
2
2
2
2
1
1
1
Host
Mouse
1302
519
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to FITC
263
219
144
86
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1638
470
374
351
290
284
264
245
215
37
33
25
23
17
14
10
7
7
6
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN371298
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN440198 FACS IHC (fro) IHC (p) IP FITC Mouse IgG2b kappa Log in to see 76-2-11
0.30226976 ABIN440197 BP FACS IHC (fro) IHC (p) IP Neut Mouse IgG2b kappa Log in to see 76-2-11
0.30226976 ABIN2161226 FACS PE Mouse IgG2a Log in to see
0.30226976 ABIN2166824 FACS FITC Mouse IgG2a Log in to see
0.30226976 ABIN2472766 FACS PE Mouse IgG2a Log in to see MIL12 4
0.0010482287 ABIN135478 FACS IP IHC Biotin Mouse IgG2a Log in to see 76-2-11 1
0.0010482287 ABIN135477 FACS IHC FITC Mouse IgG2a Log in to see 76-2-11 2
0.0010482287 ABIN135479 FACS IHC PE Mouse IgG2a Log in to see 76-2-11 1
0.0010482287 ABIN289071 FACS IHC PE Mouse IgG2a Log in to see 76-2-11
0.0010482287 ABIN289072 FACS IHC SPRD Mouse IgG2a Log in to see 76-2-11
0.0010482287 ABIN289070 FACS IP IHC Biotin Mouse IgG2a Log in to see 76-2-11
0.0010482287 ABIN371297 FACS Biotin Mouse IgG2a Log in to see 11-02-76 3
0.0010482287 ABIN135476 FACS IP IHC Mouse IgG2a Log in to see 76-2-11 1
0.0010482287 ABIN371300 FACS PE Mouse IgG2a Log in to see 11-02-76 3
0.0010482287 ABIN284333 FACS IP IHC Mouse IgG2a Log in to see 76-2-11
0.0010482287 ABIN371299 CyTox FACS IP Mouse IgG2a Log in to see 11-02-76 3
0.0010482287 ABIN320352 FACS PE Mouse IgG2a AA 3-63 Log in to see MIL-12
0.0010482287 ABIN320351 FACS FITC Mouse IgG2a AA 3-63 Log in to see MIL-12
0.0010482287 ABIN2229249 FACS IP PE Mouse IgG2a kappa Log in to see 5K107
0.0010482287 ABIN2229223 FACS PE Mouse IgG2a Log in to see 3H463

Top referenced anti-CD8 antibody for Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Pig (Porcine)
Cytotoxicity Test (CyTox) 1 Antibodies
Flow Cytometry (FACS) 42 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunoprecipitation (IP) 25 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Pig (Porcine)
(1069), (538), (168), (73), (47), (41), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (8), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(1302), (519), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to FITC
(263), (219), (144), (86), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1638), (470), (374), (351), (290), (284), (264), (245), (215), (37), (33), (25), (23), (17), (14), (10), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN371298) Images
Specificity This antibody reacts with Porcine CD8 alpha (Mr. 32-34 kDa). (Ref-1-3)
Characteristics Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain
Clone 11-02-76
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN371298) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. CD8 is expressed on most thymocytes and approximately 1/3 of peripheral blood T cells in humans. CD8 alpha/beta heterodimers are expressed only on TCR alpha/beta T cells, whereas CD8 alpha homodimers can be expressed on alpha/beta and gamma/delta T cells and some NK cells in humans. The pattern of expression in other species has not been well documented.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 396627
UniProt Q6R2U9
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN371298) Images back to top
Application Notes Flow Cytometry: < / = 1 μg/10^6 cells. (Ref.1-3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN371298) Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CD8 Antibody (ABIN371298)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Yamada, Sachs, DerSimonian: "Human anti-porcine xenogeneic T cell response. Evidence for allelic specificity of mixed leukocyte reaction and for both direct and indirect pathways of recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 11, pp. 5249-56, 1995

Smith, Sablinski, Arn, Myers, Rosengard, Uckun, Sachs: "In vivo treatment with monoclonal antibodies directed against CD4 and CD8 antigens in miniature swine." in: Journal of immunotherapy with emphasis on tumor immunology : official journal of the Society for Biological Therapy, Vol. 16, Issue 2, pp. 105-14, 1995

Pescovitz, Lunney, Sachs: "Preparation and characterization of monoclonal antibodies reactive with porcine PBL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 1, pp. 368-75, 1984

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN371298) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (FITC) (ABIN371298) anti-CD8a Molecule (CD8A) antibody (FITC)