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anti-Rat (Rattus) CD8 antibody for Immunohistochemistry (Zinc-fixed Sections)

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Rat (Rattus)
1059
532
166
68
47
40
25
22
20
20
19
16
15
13
12
12
11
11
10
10
8
8
5
3
3
2
2
2
2
1
1
1
Host
Mouse
1285
512
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
259
217
142
86
52
42
40
39
30
30
29
28
27
26
25
25
25
25
25
21
21
18
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Immunoaffinity Chromatography (IAC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1617
451
356
342
284
282
254
238
214
37
33
25
23
17
13
10
7
6
6
5
5
4
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN967471
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008696448 ABIN2689435 BR IAC IHC (f) IHC (fro) FACS IHC (zinc) IHC (p) IP WB Mouse IgG1, kappa Log in to see OX 10

Top referenced anti-CD8 antibody for Immunohistochemistry (Zinc-fixed Sections)

Similar anti-CD8 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Reagent (BR) 2 Antibodies
ELISA 3 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Flow Cytometry (FACS) 123 Antibodies
Functional Studies (Func) 7 Antibodies
Immunoaffinity Chromatography (IAC) 2 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 21 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 18 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 58 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 2 Antibodies
Immunoprecipitation (IP) 26 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Rat (Rattus)
(1059), (532), (166), (68), (47), (40), (25), (22), (20), (20), (19), (16), (15), (13), (12), (12), (11), (11), (10), (10), (8), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(1285), (512), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(259), (217), (142), (86), (52), (42), (40), (39), (30), (30), (29), (28), (27), (26), (25), (25), (25), (25), (25), (21), (21), (18), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunoaffinity Chromatography (IAC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1617), (451), (356), (342), (284), (282), (254), (238), (214), (37), (33), (25), (23), (17), (13), (10), (7), (6), (6), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 16 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN967471) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen High-molecular-weight rat thymocyte glycoproteins
Clone OX-8
Isotype IgG1, kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN967471) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Background The OX-8 antibody reacts with the hinge-like membrane-proximal domain of the 32 kDa alpha chain of the CD8 differentiation antigen. A truncated CD8 alpha' isoform has not been detected in the rat. The CD8 alpha and beta chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and a subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells). Intestinal intrapithelial lymphocytes, many CD8+ T cells of athymic rats, many activated CD4+ T cells, and most NK cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to thymus-derived T lymphocytes. OX-8 antibody does not react with resting CD4+ T helper cells. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase Ick. Macrophages have also been reported to express CD8 alpha and beta chains, which are involved in signal transduction. Soluble OX-8 mAb partially blocks in vitro MLR and CTL activity. This antibody is routinely tested by flow cytometric analysis.
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN967471) Images back to top
Application Notes For IHC, we recommend the use of purified OX-8 mAb in our special formulation for immunohistochemistry.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN967471) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.

References for anti-CD8 Antibody (ABIN967471)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Hirji, Lin, Bissonnette, Belosevic, Befus: "Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 6004-11, 1998

Kühnlein, Park, Herrmann, Elbe, Hünig et al.: "Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 979-86, 1994

Classon, Brown, Garnett, Somoza, Barclay, Willis, Williams: "The hinge region of the CD8 alpha chain: structure, antigenicity, and utility in expression of immunoglobulin superfamily domains." in: International immunology, Vol. 4, Issue 2, pp. 215-25, 1992

Product cited in:

Mitnacht, Bischof, Torres-Nagel, Hünig: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998

Scriba, Grau, Steiniger: "Phenotype of rat monocytes during acute kidney allograft rejection: increased expression of NKR-P1 and reduction of CD43." in: Scandinavian journal of immunology, Vol. 47, Issue 4, pp. 332-42, 1998

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, 1997

Wallgren, Karlsson-Parra, Korsgren: "The main infiltrating cell in xenograft rejection is a CD4+ macrophage and not a T lymphocyte." in: Transplantation, Vol. 60, Issue 6, pp. 594-601, 1995

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992

Stitz, Sobbe, Bilzer: "Preventive effects of early anti-CD4 or anti-CD8 treatment on Borna disease in rats." in: Journal of virology, Vol. 66, Issue 6, pp. 3316-23, 1992

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, 1992

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989

Johnson, Gagnon, Barclay, Williams: "Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes." in: The EMBO journal, Vol. 4, Issue 10, pp. 2539-45, 1985

Thomas, Green: "Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens." in: European journal of immunology, Vol. 13, Issue 10, pp. 855-8, 1984

Mason, Arthur, Dallman, Green, Spickett, Thomas: "Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies." in: Immunological reviews, Vol. 74, pp. 57-82, 1983

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981

Brideau, Carter, McMaster, Mason, Williams: "Two subsets of rat T lymphocytes defined with monoclonal antibodies." in: European journal of immunology, Vol. 10, Issue 8, pp. 609-15, 1980

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN967471) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN967471) The expression of CD8a on rat splenocytes. Single-cell suspensions of Lewis splenocyt...
 image for anti-CD8 antibody (CD8a Molecule) (ABIN967471) Stained with purified mAb OX-8