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anti-Human CD80 antibody for Intracellular Flow Cytometry

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • B7
  • B7-1
  • B7.1
  • B71
  • BB1
  • CD8
  • Cd28l
  • CD28LG
  • CD28LG1
  • LAB7
  • Leu2
  • Ly-53
  • Ly53
  • MAL
  • MIC17
  • p32
  • TSA1
Reactivity
Human
313
222
50
17
10
9
8
3
2
2
1
Host
Mouse
287
109
98
59
16
14
Clonality
Monoclonal
Conjugate
This CD80 antibody is conjugated to Biotin
67
65
40
26
11
10
8
7
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC)
366
173
114
106
48
44
25
22
22
18
14
7
7
7
7
5
4
4
3
2
2
2
1
1
1
1
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Catalog No. ABIN2661134
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000873515 ABIN2658720 FACS ICFC APC Mouse IgG1 kappa Log in to see 2D10 9
0.000873515 ABIN2658721 FACS ICFC APC Mouse IgG1 kappa Log in to see 2D10 9

Top referenced anti-CD80 antibody for Intracellular Flow Cytometry

Similar anti-CD80 Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
ELISA 94 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 158 Antibodies
Functional Studies (Func) 8 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 38 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 23 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 53 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Luminex Assay (LMNX) 5 Antibodies
Neutralization (Neut) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 145 Antibodies

General

Antigen CD80 Antibodies
Reactivity Human
(313), (222), (50), (17), (10), (9), (8), (3), (2), (2), (1)
Host Mouse
(287), (109), (98), (59), (16), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD80 antibody is conjugated to Biotin
(67), (65), (40), (26), (11), (10), (8), (7), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(366), (173), (114), (106), (48), (44), (25), (22), (22), (18), (14), (7), (7), (7), (7), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN2661134) Images
Cross-Reactivity Rhesus Monkey
Purification The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Clone 2D10
Isotype IgG1 kappa

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 Antibody (ABIN2661134) Images back to top
Antigen
Alternative Name CD80 (CD80 Antibody Abstract)
Background CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 Antibody (ABIN2661134) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 Antibody (ABIN2661134) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD80 Antibody (ABIN2661134)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Background publications

Lee, Breton, Oliveira, Zhou, Aljoufi, Puhr, Cameron, Sékaly, Nussenzweig, Liu: "Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow." in: The Journal of experimental medicine, Vol. 212, Issue 3, pp. 385-99, 2015

Schubert, Gordo, Sabatino, Vardhana, Gagnon, Sethi, Seth, Choudhuri, Reijonen, Nepom, Evavold, Dustin, Wucherpfennig: "Self-reactive human CD4 T cell clones form unusual immunological synapses." in: The Journal of experimental medicine, Vol. 209, Issue 2, pp. 335-52, 2012

Jayakumar, Donovan, Tripathi, Ramalho-Ortigao, McDowell: "Leishmania major infection activates NF-kappaB and interferon regulatory factors 1 and 8 in human dendritic cells." in: Infection and immunity, Vol. 76, Issue 5, pp. 2138-48, 2008

Battifora, Pesce, Paolieri, Fiorino, Giordano, Riccio, Torre, Olive, Bagnasco: "B7.1 costimulatory molecule is expressed on thyroid follicular cells in Hashimoto's thyroiditis, but not in Graves' disease." in: The Journal of clinical endocrinology and metabolism, Vol. 83, Issue 11, pp. 4130-9, 1998

van der Merwe, Bodian, Daenke, Linsley, Davis: "CD80 (B7-1) binds both CD28 and CTLA-4 with a low affinity and very fast kinetics." in: The Journal of experimental medicine, Vol. 185, Issue 3, pp. 393-403, 1997

Linsley, Bradshaw, Greene, Peach, Bennett, Mittler: "Intracellular trafficking of CTLA-4 and focal localization towards sites of TCR engagement." in: Immunity, Vol. 4, Issue 6, pp. 535-43, 1996

Linsley, Brady, Urnes, Grosmaire, Damle, Ledbetter: "CTLA-4 is a second receptor for the B cell activation antigen B7." in: The Journal of experimental medicine, Vol. 174, Issue 3, pp. 561-9, 1991

Freeman, Gray, Gimmi, Lombard, Zhou, White, Fingeroth, Gribben, Nadler: "Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7." in: The Journal of experimental medicine, Vol. 174, Issue 3, pp. 625-31, 1991

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN2661134) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD80 antibody (CD80)  (Biotin) (ABIN2661134) anti-CD80 (CD80) antibody (Biotin)