anti-Rat (Rattus) Cytoplasmic Protein NCK1 antibody for Immunofluorescence

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • NCK1
  • NCK
  • NCKalpha
  • nck-1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK1
  • LOC100347965
  • LOC100358703
  • Nck1
Epitope
AA 279-377
29
26
14
5
4
3
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
146
31
25
18
8
4
4
3
3
2
2
2
2
2
1
1
1
1
Host
Mouse
76
69
1
Clonality
Monoclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
6
6
6
6
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
139
89
61
26
14
14
7
5
4
4
1
1
1
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Catalog No. ABIN967743
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.176395 ABIN967744 IF IP IHC WB Mouse IgG2b AA 279-377 Log in to see 108-NCK 5
11.176395 ABIN3197734 IC IF WB Rabbit Log in to see Polyclonal
1 ABIN1873839 IF IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2888058 IF IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1089241 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1089242 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Epitope AA 279-377
(29), (26), (14), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(146), (31), (25), (18), (8), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(76), (69), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(6), (6), (6), (6), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(139), (89), (61), (26), (14), (14), (7), (5), (4), (4), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967743) Images
Cross-Reactivity Cow (Bovine), Chicken, Dog (Canine), Frog, Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human NCK aa. 279-377
Clone 108-NCK
Isotype IgG2b

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967743) Images back to top
Antigen
Alternative Name NCK (NCK1 Antibody Abstract)
Background NCK is a 47 kDa protein that belongs to a class of signaling molecules called adaptors. These proteins exert their effects by coupling activated receptors to downstream effectors. NCK is comprised almost entirely of one SH2 and three SH3 domains and it possesses no intrinsic catalytic activity. Investigations of its interactions with cellular proteins have found constitutive association of NCK with various Ser/THr kinases, such as p21-associated kinase (PAK) and casein kinase 1. Other proteins that directly interact with NCK are: Sos, Cbl, Wiskott-Aldrich syndrome protein (WASP), IRS1, and multiple tyrosine kinase receptors. The presence of NCK is necessary for the mitogenic response elicited by PDGF in some cells. In addition, the Drosophila homologue of NCK is involved in regulating cytoskeletal organization. This antibody is routinely tested by western blot analysis.
Molecular Weight 47 kDa
Pathways TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967743) Images back to top
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967743) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967743)

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling Images back to top
Product cited in:

Dalva, Takasu, Lin, Shamah, Hu, Gale, Greenberg: "EphB receptors interact with NMDA receptors and regulate excitatory synapse formation." in: Cell, Vol. 103, Issue 6, pp. 945-56, 2001

Miyoshi-Akiyama, Aleman, Smith, Adler, Mayer: "Regulation of Cbl phosphorylation by the Abl tyrosine kinase and the Nck SH2/SH3 adaptor." in: Oncogene, Vol. 20, Issue 30, pp. 4058-69, 2001

Schmitz, Thömmes, Beier, Wagner, Sachinidis, Düsing, Vetter: "Angiotensin II-induced stimulation of p21-activated kinase and c-Jun NH2-terminal kinase is mediated by Rac1 and Nck." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22003-10, 2001

McManus, Boerner, Danielsen, Wang, Matsumura, Maihle: "An oncogenic epidermal growth factor receptor signals via a p21-activated kinase-caldesmon-myosin phosphotyrosine complex." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35328-34, 2000

Roche, McGlade, Jones, Gish, Pawson, Courtneidge et al.: "Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent ..." in: The EMBO journal, Vol. 15, Issue 18, pp. 4940-8, 1996

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967743) back to top
Supplier Images
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967743) Western blot analysis of NCK on HeLa lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: ...
Immunofluorescence (IF) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967743) Immunofluorescent staining of HISM cells with anti-NCK antibody.