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anti-Rat (Rattus) Cytoplasmic Protein NCK1 antibody for Immunohistochemistry

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • NCK
  • Nck-1
  • nck-1
  • NCK1
  • NCKalpha
Reactivity
Human, Mouse (Murine), Rat (Rattus)
96
19
17
3
2
2
2
2
Host
Rabbit
71
25
1
Clonality
Monoclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
4
4
4
4
4
4
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
91
52
41
13
10
8
7
3
2
1
1
Supplier
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Available images

Catalog No. ABIN189565
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22054756 ABIN2428459 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22054756 ABIN2421903 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.001136324 ABIN967743 IF IP IHC WB Mouse IgG2b AA 279-377 Log in to see 108-NCK 5
0.001136324 ABIN967744 IF IP IHC WB Mouse IgG2b AA 279-377 Log in to see 108-NCK 5
0.001136324 ABIN1873840 IHC WB Rabbit IgG Log in to see Polyclonal
0.001136324 ABIN1873839 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.001136324 ABIN3021268 IHC WB Rabbit IgG Log in to see Polyclonal
0.001136324 ABIN2403762 IHC WB Rabbit IgG Log in to see Polyclonal
0.001136324 ABIN2471526 IHC ELISA WB Mouse IgG1 kappa Log in to see 11D10
0.001136324 ABIN1089241 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.001136324 ABIN1089242 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.001136324 ABIN2753934 IHC ELISA WB Rabbit IgG AA 58-377 Log in to see Polyclonal
0.001136324 ABIN2989000 IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Cytoplasmic Protein NCK1 antibody for Immunohistochemistry

Similar anti-Cytoplasmic Protein NCK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 7 Antibodies 6 Antibodies 52 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 3 Antibodies
Immunofluorescence (IF) 7 Antibodies 8 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 3 Antibodies
Immunoprecipitation (IP) 4 Antibodies 3 Antibodies
Western Blotting (WB) 17 Antibodies 19 Antibodies

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(96), (19), (17), (3), (2), (2), (2), (2)
Host Rabbit
(71), (25), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(91), (52), (41), (13), (10), (8), (7), (3), (2), (1), (1)
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling Images
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues close to the SH2 domain of human Nck was used as immunogen.
Clone Y531
Isotype IgG

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derivedgrowth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonineresidues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 ratfibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 andVAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linkingtyrosine and serine/threonine kinase pathways within the cell (2). Alternate Names: anti-Cytoplasmic protein NCK1 antibody, anti-Melanoma Nck protein antibody,anti-MGC12668 antibody, anti-NCK 1 antibody, anti-Nck adaptor protein 1 antibody,anti-NCK tyrosine kinase antibody, anti-NCK1 antibody, anti-NCKalpha antibody,anti-Non catalytic region of tyrosine kinase antibody, anti-SH2/SH3 adaptor protein NCKalpha antibody, anti-SH2/SH3 adaptor protein NCKalpha antibody.
Gene Symbol: NCK1
Gene ID 4690, 17973, 300955
Pathways TCR Signaling

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 47kDa.
Recommended dilutions: Flow Cytometry 1:60, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:50, Western Blot 1:500-10000
Protocol Protocol specific for Nck Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (ABIN189565) Immunofluorescent staining of HeLa cells using anti- NAK/TBK1 (N-term) RabMAb (cat. #...
Western Blotting (WB) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (ABIN189565) Western blot analysis on Hela cell lysate using anti-Nck RabMAb 1:10,000 dilution.