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anti-Sheep (Ovine) GATA3 antibody for Western Blotting

Recommended GATA3 Antibody (supplied by: Log in to see )

Antigen
GATA Binding Protein 3 (GATA3) Antibodies
  • GATA-3
  • XGATA3
  • hdr
  • xGATA-3
  • GATA3
  • gata3
  • HDR
  • HDRS
  • xgata-3
  • Gata-3
  • jal
  • cb550
  • gta3
  • GATA binding protein 3
  • amidase
  • putative amidase
  • transcription factor GATA-3
  • GATA-binding protein 3
  • gata3
  • GATA3
  • gatA3
  • Gata3
Epitope
AA 36-85
25
18
15
14
11
11
10
9
8
8
7
7
7
4
3
3
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish
208
116
47
13
13
8
8
4
3
3
3
1
1
1
1
1
1
Host
Rabbit
114
73
20
7
Clonality
Polyclonal
Conjugate
This GATA3 antibody is un-conjugated
8
5
5
5
5
4
4
4
3
2
2
2
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
151
86
64
32
29
27
26
11
6
6
2
1
1
1
Supplier
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Catalog No. ABIN462169
$ 676.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN604963 IHC (p) ELISA WB Goat AA 2-14 Log in to see Polyclonal

Similar anti-GATA3 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Cow (Bovine) Pig (Porcine) Zebrafish Dog (Canine) Rabbit Sheep (Ovine) Monkey Hamster Horse (Equine)
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies 6 Antibodies 1 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 147 Antibodies 9 Antibodies 14 Antibodies 9 Antibodies 66 Antibodies 14 Antibodies 35 Antibodies 5 Antibodies 4 Antibodies 2 Antibodies 4 Antibodies 2 Antibodies 2 Antibodies

General

Antigen GATA Binding Protein 3 (GATA3) Antibodies
Epitope AA 36-85
(25), (18), (15), (14), (11), (11), (10), (9), (8), (8), (7), (7), (7), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish
(208), (116), (47), (13), (13), (8), (8), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Host Rabbit
(114), (73), (20), (7)
Clonality
Polyclonal
Conjugate This GATA3 antibody is un-conjugated
(8), (5), (5), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(151), (86), (64), (32), (29), (27), (26), (11), (6), (6), (2), (1), (1), (1)
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Product Details anti-GATA3 Antibody

Target Details GATA3 Application Details Handling Images
Brand IHC-plus™
Specificity Human GATA3
Predicted Reactivity Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Guinea pig (92%) Zebrafish (84%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide located between aa36-85 of human GATA3 (P23771, NP_002042). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Zebra finch, Chicken (100%), Bat, Guinea pig, Trout, Salmon (92%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details GATA3

Product Details anti-GATA3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GATA3 (GATA3 Antibody Abstract)
Background Name/Gene ID: GATA3
Family: Transcription factor

Synonyms: GATA3, GATA-binding factor 3, HDRS, GATA binding protein 3, GATA-binding protein 3, HDR
Gene ID 2625
NCBI Accession NP_002042
UniProt P23771
Research Area Lineage Markers, Transcription Factors, Adaptive Immunity
Pathways TCR Signaling, Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation

Application Details

Product Details anti-GATA3 Antibody Target Details GATA3 Handling Images back to top
Application Notes Approved: IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Restrictions For Research Use only

Handling

Product Details anti-GATA3 Antibody Target Details GATA3 Application Details Images back to top
Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.

Images

Product Details anti-GATA3 Antibody Target Details GATA3 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-GATA3 antibody (GATA Binding Protein 3) (AA 36-85) (ABIN462169) Human Kidney (formalin-fixed, paraffin-embedded) stained with GATA3 antibody ABIN4621...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-GATA3 antibody (GATA Binding Protein 3) (AA 36-85) (ABIN462169) Human Testis (formalin-fixed, paraffin-embedded) stained with GATA3 antibody ABIN4621...