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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||3 Antibodies|
|Blocking Antibody (Inhibition)||3 Antibodies|
|Blocking Reagent (BR)||2 Antibodies|
|Confocal Microscopy (CM)||2 Antibodies|
|Depletion (Dep)||1 Antibodies|
|ELISA (Capture)||7 Antibodies|
|ELISA (Detection)||8 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||3 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Enzyme Immunoassay (EIA)||11 Antibodies|
|Flow Cytometry (FACS)||78 Antibodies|
|Functional Studies (Func)||9 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||10 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||30 Antibodies|
|Antigen||Interleukin 17A (IL17A) Antibodies|
|Conjugate||This IL17A antibody is un-conjugated Alternatives|
Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Product Details anti-IL17A AntibodyTarget Details IL17A Application Details Handling Images
|Characteristics||Polyclonal antibody to detect IL-17A in human samples|
|Immunogen||E.coli derived Recombinant Human IL-17A|
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|Alternative Name||IL-17A (IL17A Antibody Abstract)|
The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 136 amino acid chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells.
Synonyms: CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte associated antigen 8
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WB: Use 0.1-0.2 μg/mL. The detection limit for recombinant human IL-17A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA: Use 0.5 - 2.0 μg/mL (100 μL/well antibody solution)
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17A (50.0 ng/mL), a concentration of 0.9-1.3 μg/mL of this antibody is required.
Immunohistochemistry: 0.25 μg/mL with an overnight incubation at 4 °C.
Suggested positive control: ELISA: Recombinant hIL-17A (Cat # 4176) IHC: Human breast invasive ductal carcinoma tissue section
|Restrictions||For Research Use only|
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|Buffer||A sterile filtered antibody solution in PBS, pH 7.2.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Expiry Date||12 months|
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