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|Antigen||Interleukin 17A (IL17A) Antibodies|
|Conjugate||This IL17A antibody is un-conjugated Alternatives|
Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Product Details anti-IL17A AntibodyTarget Details IL17A Application Details Handling Images
|Purpose||Polyclonal antibody to detect IL-17A in human samples|
|Characteristics||Polyclonal antibody to detect IL-17A in human samples|
|Immunogen||E.coli derived Recombinant Human IL-17A|
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|Alternative Name||IL-17A (IL17A Antibody Abstract)|
The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 136 amino acid chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells.
Synonyms: CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte associated antigen 8
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1) WB: Use 0.1-0.2 μg/mL. The detection limit for recombinant human IL-17A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
2) ELISA: Use 0.5 - 2.0 μg/mL (100 μL/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17A (50.0 ng/mL), a concentration of 0.9-1.3 μg/mL of this antibody is required.
4) Immunohistochemistry: 0.25 μg/mL with an overnight incubation at 4 °C.
Suggested positive control:
|Restrictions||For Research Use only|
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|Buffer||A sterile filtered antibody solution in PBS, pH 7.2.|
|Handling Advice||The antibody solution should be gently mixed before use.|
|Expiry Date||12 months|
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