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anti-Mouse (Murine) IL17A antibody for Flow Cytometry

Recommended IL17A Antibody (supplied by: Log in to see )

Antigen
Interleukin 17A (IL17A) Antibodies
  • Il17
  • IL17A
  • CTLA8
  • IL-17
  • IL-17A
  • IL17
  • Ctla-8
  • Ctla8
  • CTLA-8
  • ChIL-17
  • IL-17F
  • interleukin 17A
  • interleukin 17A-like
  • interleukin 17F
  • Il17a
  • IL17A
  • LOC100339322
  • IL17F
Epitope
AA 22-158
32
30
15
15
14
14
13
11
8
8
7
6
6
4
4
4
4
2
2
1
1
1
1
1
Reactivity
Mouse (Murine)
431
187
62
10
5
5
1
Host
Goat
244
207
101
50
8
3
1
Clonality
Polyclonal
Conjugate
This IL17A antibody is un-conjugated
70
35
32
24
17
15
15
12
10
10
8
7
7
7
7
7
7
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
340
319
199
57
40
40
22
21
15
12
10
9
9
8
8
6
5
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN4899637
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.739031 ABIN4896412 FACS PE Goat IgG AA 22-158 Log in to see Polyclonal 2
13.739031 ABIN2663988 FACS PE Rat IgG1 kappa Log in to see TC11-18H10-1 10
13.739031 ABIN2658881 FACS APC Rat IgG1 kappa Log in to see TC11-18H10-1 11
13.739031 ABIN2657538 FACS Alexa Fluor 488 Rat IgG1 kappa Log in to see TC11-18H10-1 11
13.739031 ABIN2658105 FACS Alexa Fluor 700 Rat IgG1 kappa Log in to see TC11-18H10-1 11
13.739031 ABIN2659588 FACS IF WB PE-Cy7 Rat IgG1 kappa Log in to see TC11-18H10-1 11
13.739031 ABIN2660444 FACS PerCP-Cy5.5 Rat IgG1 kappa Log in to see TC11-18H10-1 11
13.739031 ABIN2657944 FACS Alexa Fluor 647 Rat IgG1 kappa Log in to see TC11-18H10-1 11
13.739031 ABIN2662100 FACS Pacific Blue Rat IgG1 kappa Log in to see TC11-18H10-1 11
13.739031 ABIN2665115 FACS Rat IgG1 kappa Log in to see TC11-18H10-1 11
13.739031 ABIN4896408 FACS Alexa Fluor 488 Rat IgG2b AA 22-158 Log in to see 881309
13.739031 ABIN4896410 FACS PerCP Goat IgG AA 22-158 Log in to see Polyclonal
13.739031 ABIN4896411 FACS Fluorescein Goat IgG AA 22-158 Log in to see Polyclonal
13.739031 ABIN4324635 ELISA FACS WB Rat IgG2a kappa Log in to see 20B4-G10-F5
13.739031 ABIN4896409 FACS Alexa Fluor 647 Rat IgG2b AA 22-158 Log in to see 881309
13.739031 ABIN4899638 FACS Rat IgG2b AA 22-158 Log in to see 881309
1 ABIN738891 FACS IF (p) IHC (p) Rabbit IgG AA 110-150 Log in to see Polyclonal 3
1 ABIN4324630 ELISA FACS IHC IHC (p) WB Rat IgG1 Log in to see TC11-18H10 2
1 ABIN738898 FACS IF (p) FITC Rabbit IgG AA 110-150 Log in to see Polyclonal 1
1 ABIN2661210 FACS Biotin Rat IgG1 kappa Log in to see TC11-8H4 10

Top referenced anti-IL17A antibody for Flow Cytometry

Similar anti-IL17A Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Confocal Microscopy (CM) 2 Antibodies
ELISA 107 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 82 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 5 Antibodies

General

Antigen Interleukin 17A (IL17A) Antibodies
Epitope AA 22-158
(32), (30), (15), (15), (14), (14), (13), (11), (8), (8), (7), (6), (6), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(431), (187), (62), (10), (5), (5), (1)
Host Goat
(244), (207), (101), (50), (8), (3), (1)
Clonality
Polyclonal
Conjugate This IL17A antibody is un-conjugated
(70), (35), (32), (24), (17), (15), (15), (12), (10), (10), (8), (7), (7), (7), (7), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
(340), (319), (199), (57), (40), (40), (22), (21), (15), (12), (10), (9), (9), (8), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-IL17A Antibody

Target Details IL17A Application Details Handling References for anti-IL17A Antibody (ABIN4899637) Images
Specificity Detects mouse and rat IL-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10 % cross-reactivity with recombinant human IL-17A and recombinant mouse (rm) IL-17F is observed and less than 1 % cross-reactivity with rmIL-17B, rmIL-17C, rmIL-17D, and rmIL-17E is observed.
Purification Immunogen affinity purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 g of the antibody by the LAL method.
Immunogen E. coli-derived recombinant mouse IL-17, Thr22-Ala158, Accession # Q62386
Isotype IgG

Target Details IL17A

Product Details anti-IL17A Antibody Application Details Handling References for anti-IL17A Antibody (ABIN4899637) Images back to top
Antigen
Alternative Name IL-17/IL-17A (IL17A Antibody Abstract)
Gene ID 3605
UniProt Q62386
Pathways TCR Signaling

Application Details

Product Details anti-IL17A Antibody Target Details IL17A Handling References for anti-IL17A Antibody (ABIN4899637) Images back to top
Application Notes Western Blot 0.1 μg/mL, Neutralization 0.05-0.25 μg/mL, Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells, CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-IL17A Antibody Target Details IL17A Application Details References for anti-IL17A Antibody (ABIN4899637) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer 0.2 m filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 C as supplied. 1 month, 2 to 8 C under sterile conditions after reconstitution. 6 months, -20 to -70 C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-IL17A Antibody (ABIN4899637)

Product Details anti-IL17A Antibody Target Details IL17A Application Details Handling Images back to top
Product cited in:

Benevides, da Fonseca, Donate, Tiezzi, De Carvalho, de Andrade, Martins, Silva: "IL17 Promotes Mammary Tumor Progression by Changing the Behavior of Tumor Cells and Eliciting Tumorigenic Neutrophils Recruitment." in: Cancer research, Vol. 75, Issue 18, pp. 3788-99, 2015 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Carmi, Rinott, Dotan, Elkabets, Rider, Voronov, Apte: "Microenvironment-derived IL-1 and IL-17 interact in the control of lung metastasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 6, pp. 3462-71, 2011 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Li, Burns, Han, Rumbaut, Smith: "IL-17 and VEGF are necessary for efficient corneal nerve regeneration." in: The American journal of pathology, Vol. 178, Issue 3, pp. 1106-16, 2011 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Otani, Watanabe, Tanigawa, Okazaki, Yamagami, Watanabe, Tominaga, Fujiwara, Oshitani, Arakawa: "Anti-inflammatory effects of IL-17A on Helicobacter pylori-induced gastritis." in: Biochemical and biophysical research communications, Vol. 382, Issue 2, pp. 252-8, 2009 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Yuan, Ansari, DAddio, Paez-Cortez, Schmitt, Donnarumma, Boenisch, Zhao, Popoola, Clarkson, Yagita, Akiba, Freeman, Iacomini, Turka, Glimcher, Sayegh: "Targeting Tim-1 to overcome resistance to transplantation tolerance mediated by CD8 T17 cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 26, pp. 10734-9, 2009 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Lemos, Grespan, Vieira, Cunha, Verri, Fernandes, Souto, McInnes, Ferreira, Liew, Cunha: "Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 14, pp. 5954-9, 2009 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Muranski, Boni, Antony, Cassard, Irvine, Kaiser, Paulos, Palmer, Touloukian, Ptak, Gattinoni, Wrzesinski, Hinrichs, Kerstann, Feigenbaum, Chan, Restifo: "Tumor-specific Th17-polarized cells eradicate large established melanoma." in: Blood, Vol. 112, Issue 2, pp. 362-73, 2008 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Venkatachalam, Mummidi, Cortez, Prabhu, Valente, Chandrasekar: "Resveratrol inhibits high glucose-induced PI3K/Akt/ERK-dependent interleukin-17 expression in primary mouse cardiac fibroblasts." in: American journal of physiology. Heart and circulatory physiology, Vol. 294, Issue 5, pp. H2078-87, 2008 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Gocke, Cravens, Ben, Hussain, Northrop, Racke, Lovett-Racke: "T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 3, pp. 1341-8, 2007 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Ferretti, Bonneau, Dubois, Jones, Trifilieff: "IL-17, produced by lymphocytes and neutrophils, is necessary for lipopolysaccharide-induced airway neutrophilia: IL-15 as a possible trigger." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 4, pp. 2106-12, 2003 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Images

Product Details anti-IL17A Antibody Target Details IL17A Application Details Handling References for anti-IL17A Antibody (ABIN4899637) back to top
Supplier Images
Neutralization (Neut) image for anti-IL17A antibody (Interleukin 17A) (AA 22-158) (ABIN4899637) IL-6 Secretion Induced by IL-17 and Neutralization by Mouse IL-17 Antibody. Recombina...
Flow Cytometry (FACS) image for anti-IL17A antibody (Interleukin 17A) (AA 22-158) (ABIN4899637) Detection of IL-17 in EL-4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast ...