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anti-Human PKC theta antibody for Intracellular Staining

Recommended PKC theta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, theta (PRKCQ) Antibodies
  • A130035A12Rik
  • AW494342
  • nPKC-theta
  • PKC-0
  • PKC-theta
  • Pkcq
  • PKCtheta
  • PRKCT
Epitope
AA 21-217
41
41
37
10
9
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
194
117
108
4
4
4
4
Host
Mouse
203
6
3
1
Clonality
Monoclonal
Conjugate
This PKC theta antibody is conjugated to PE
5
5
5
3
3
3
3
3
3
3
3
3
2
2
2
Application
Intracellular Staining (ICS)
151
107
72
34
32
29
5
3
2
2
1
1
Supplier
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Catalog No. ABIN1176929
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Top referenced anti-PKC theta antibody for Intracellular Staining

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Application / Reactivity Human
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Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 33 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 106 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
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Western Blotting (WB) 145 Antibodies

General

Antigen Protein Kinase C, theta (PRKCQ) Antibodies
Epitope AA 21-217
(41), (41), (37), (10), (9), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(194), (117), (108), (4), (4), (4), (4)
Host Mouse
(203), (6), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC theta antibody is conjugated to PE
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Intracellular Staining (ICS)
(151), (107), (72), (34), (32), (29), (5), (3), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-PKC theta Antibody

Target Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN1176929) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human PKCtheta aa. 21-217 Recombinant Protein
Clone 27-PKCtheta
Isotype IgG2a, kappa

Target Details PKC theta

Product Details anti-PKC theta Antibody Application Details Handling References for anti-PKC theta Antibody (ABIN1176929) Images back to top
Antigen
Alternative Name PKCq (PRKCQ Antibody Abstract)
Background The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes, such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. Conventional PKC (cPKC) subfamily members (a, b, and gamma isoforms) consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the the C2-like domains of novel PKC (nPKC) subfamily members (delta, epsilon, eta, and theta isoforms) are Ca2+-independent. The atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) lack the C2 domain and are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC members. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. PKCtheta transcripts are expressed in most tissues with the highest levels being found in hematopoietic tissues and cell lines, including T cells and thymocytes. PKCtheta mRNA is readily detectable in skeletal muscle, lung and brain. However, PKCtheta expression is not detected in several human carcinoma cell lines. Abundant expression of this PKC isozyme in hematopoietic cells suggest that it may have a role in growth and differentiation processes of these cells. The 27/PKCtheta monoclonal antibody recognizes the C2-like domain of human PKCtheta, regardless of phosphorylation status.
Synonyms: KPCT, nPKC-theta, PRKCQ, PRKCT
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-PKC theta Antibody Target Details PKC theta Handling References for anti-PKC theta Antibody (ABIN1176929) Images back to top
Application Notes Either BD Cytofix™ Fixation Buffer or BD Phosflow™ Fix Buffer I may be used for cell fixation. Any of the three BD Phosflow™ permeabilization buffers may be used.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details References for anti-PKC theta Antibody (ABIN1176929) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

References for anti-PKC theta Antibody (ABIN1176929)

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling Images back to top
Product cited in:

Parker, Murray-Rust: "PKC at a glance." in: Journal of cell science, Vol. 117, Issue Pt 2, pp. 131-2, 2003