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anti-Human PKC theta antibody for Immunofluorescence

Recommended PKC theta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, theta (PRKCQ) Antibodies
  • PRKCT
  • nPKC-theta
  • A130035A12Rik
  • AW494342
  • PKC-0
  • PKC-theta
  • PKCtheta
  • Pkcq
  • protein kinase C, theta
  • protein kinase C theta
  • PRKCQ
  • PKC
  • Prkcq
Epitope
AA 21-217
45
42
39
11
10
9
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
217
138
132
12
10
10
7
4
3
2
1
1
Host
Mouse
212
21
3
1
Clonality
Monoclonal
Conjugate
This PKC theta antibody is un-conjugated
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
160
110
70
42
40
34
15
6
5
3
2
2
2
1
1
1
Options
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Catalog No. ABIN967738
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.087689 ABIN967739 IHC (f) IF IP WB Mouse IgG2a, kappa AA 21-217 Log in to see 27-PKCtheta 5
11.087689 ABIN2705132 IC IF IHC WB Rabbit C-Term, pSer676 Log in to see Polyclonal
11.087689 ABIN2957727 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
11.087689 ABIN1870528 IF IHC WB Rabbit IgG pSer676 Log in to see Polyclonal
11.087689 ABIN4345851 ICC IF IHC IHC (p) WB Rabbit pSer676 Log in to see Polyclonal
11.087689 ABIN5078853 ICC IF Rabbit IgG Log in to see Polyclonal
11.087689 ABIN5078852 ICC IF Rabbit IgG Log in to see Polyclonal
11.087689 ABIN2425053 IF IHC WB Rabbit IgG pSer676 Log in to see Polyclonal
11.087689 ABIN3019959 IF IHC WB Rabbit pSer676 Log in to see Polyclonal
1 ABIN197070 IF IHC (p) WB Rabbit pSer676 Log in to see Polyclonal 3
1 ABIN197566 IF IHC (p) WB Rabbit AA 674-678 Log in to see Polyclonal 3
1 ABIN363004 IF IHC WB Rabbit IgG AA 674-678 Log in to see Polyclonal 3
1 ABIN362386 IF IHC WB Rabbit IgG pSer676 Log in to see Polyclonal 3
1 ABIN498699 IF IHC (p) WB Rabbit pSer676 Log in to see Polyclonal
1 ABIN498658 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN4345852 ICC IF IHC IHC (p) WB Rabbit pSer676 Log in to see Polyclonal
1 ABIN3182130 ELISA IF IHC WB Rabbit IgG pSer676 Log in to see Polyclonal
1 ABIN3186480 ELISA IF IHC WB Rabbit IgG Thr320 Log in to see Polyclonal
1 ABIN2882626 IF IHC WB Rabbit IgG pSer676 Log in to see Polyclonal
1 ABIN2879457 IF IHC (p) WB Rabbit AA 640-690 Log in to see Polyclonal

Top referenced anti-PKC theta antibody for Immunofluorescence

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Application / Reactivity Human
ELISA 68 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 15 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 35 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 27 Antibodies
Immunohistochemistry (IHC) 107 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 153 Antibodies

General

Antigen Protein Kinase C, theta (PRKCQ) Antibodies
Epitope AA 21-217
(45), (42), (39), (11), (10), (9), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(217), (138), (132), (12), (10), (10), (7), (4), (3), (2), (1), (1)
Host Mouse
(212), (21), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC theta antibody is un-conjugated
(5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(160), (110), (70), (42), (40), (34), (15), (6), (5), (3), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-PKC theta Antibody

Target Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN967738) Images
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human PKCtheta aa. 21-217
Clone 27-PKCtheta
Isotype IgG2a, kappa

Target Details PKC theta

Product Details anti-PKC theta Antibody Application Details Handling References for anti-PKC theta Antibody (ABIN967738) Images back to top
Antigen
Alternative Name PKC theta (PRKCQ Antibody Abstract)
Background The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes, such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members (delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+-independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC members. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. PKCtheta transcripts are expressed in most tissues with the highest levels being found in hematopoietic tissues and cell lines, including T cells and thymocytes. PKCtheta mRNA is readily detectable in skeletal muscle, lung and brain. However, PKCtheta expression is not detected in several human carcinoma cell lines. Abundant expression of this PKC isozyme in hematopoietic cells suggest that it may have a role in growth and differentiation processes of these cells. This antibody is routinely tested by western blot analysis.
Molecular Weight 79 kDa
Research Area Cancer
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-PKC theta Antibody Target Details PKC theta Handling References for anti-PKC theta Antibody (ABIN967738) Images back to top
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details References for anti-PKC theta Antibody (ABIN967738) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-PKC theta Antibody (ABIN967738)

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling Images back to top
Product cited in:

Villalba, Bi, Rodriguez, Tanaka, Schoenberger, Altman: "Vav1/Rac-dependent actin cytoskeleton reorganization is required for lipid raft clustering in T cells." in: The Journal of cell biology, Vol. 155, Issue 3, pp. 331-8, 2001

Dienz, Hehner, Droge, Schmitz: "Synergistic activation of NF-kappa B by functional cooperation between vav and PKCtheta in T lymphocytes." in: The Journal of biological chemistry, Vol. 275, Issue 32, pp. 24547-51, 2000

Villalba, Coudronniere, Deckert, Teixeiro, Mas, Altman: "A novel functional interaction between Vav and PKCtheta is required for TCR-induced T cell activation." in: Immunity, Vol. 12, Issue 2, pp. 151-60, 2000

Soderling: "Protein kinases. Regulation by autoinhibitory domains." in: The Journal of biological chemistry, Vol. 265, Issue 4, pp. 1823-6, 1990

Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, 1988

Images

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN967738) back to top
Supplier Images
Western Blotting (WB) image for anti-PKC theta antibody (Protein Kinase C, theta) (AA 21-217) (ABIN967738) Western blot analysis of PKCtheta on a Jurkat lysate. Lane 1: 1:250, lane 2: 1:500, l...
Immunofluorescence (IF) image for anti-PKC theta antibody (Protein Kinase C, theta) (AA 21-217) (ABIN967738) Immunofluorescence staining of A431 cells.