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anti-Human PKC theta antibody for Immunoprecipitation

Recommended PKC theta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, theta (PRKCQ) Antibodies
  • A130035A12Rik
  • AW494342
  • nPKC-theta
  • PKC-0
  • PKC-theta
  • Pkcq
  • PKCtheta
  • PRKCT
Epitope
AA 21-217
41
41
37
10
9
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
194
117
108
4
4
4
4
Host
Mouse
203
6
3
1
Clonality
Monoclonal
Conjugate
This PKC theta antibody is un-conjugated
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
150
107
72
34
31
29
4
2
2
2
1
1
1
Supplier
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Catalog No. ABIN967738
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000892236 ABIN967739 IHC (f) IF IP WB Mouse IgG2a, kappa AA 21-217 Log in to see 27-PKCtheta 5
0.000892236 ABIN1926667 IP WB Rabbit AA 350-400 Log in to see Polyclonal
0.000892236 ABIN1926668 IP WB Rabbit AA 656-706 Log in to see Polyclonal

Top referenced anti-PKC theta antibody for Immunoprecipitation

Similar anti-PKC theta Antibodies

Application / Reactivity Human
ELISA 70 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 33 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 106 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 145 Antibodies

General

Antigen Protein Kinase C, theta (PRKCQ) Antibodies
Epitope AA 21-217
(41), (41), (37), (10), (9), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(194), (117), (108), (4), (4), (4), (4)
Host Mouse
(203), (6), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC theta antibody is un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(150), (107), (72), (34), (31), (29), (4), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-PKC theta Antibody

Target Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN967738) Images
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human PKCtheta aa. 21-217
Clone 27-PKCtheta
Isotype IgG2a, kappa

Target Details PKC theta

Product Details anti-PKC theta Antibody Application Details Handling References for anti-PKC theta Antibody (ABIN967738) Images back to top
Antigen
Alternative Name PKC theta (PRKCQ Antibody Abstract)
Background The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes, such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members (delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+-independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC members. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. PKCtheta transcripts are expressed in most tissues with the highest levels being found in hematopoietic tissues and cell lines, including T cells and thymocytes. PKCtheta mRNA is readily detectable in skeletal muscle, lung and brain. However, PKCtheta expression is not detected in several human carcinoma cell lines. Abundant expression of this PKC isozyme in hematopoietic cells suggest that it may have a role in growth and differentiation processes of these cells. This antibody is routinely tested by western blot analysis.
Molecular Weight 79 kDa
Research Area Cancer
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-PKC theta Antibody Target Details PKC theta Handling References for anti-PKC theta Antibody (ABIN967738) Images back to top
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details References for anti-PKC theta Antibody (ABIN967738) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-PKC theta Antibody (ABIN967738)

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling Images back to top
Product cited in:

Villalba, Bi, Rodriguez, Tanaka, Schoenberger, Altman: "Vav1/Rac-dependent actin cytoskeleton reorganization is required for lipid raft clustering in T cells." in: The Journal of cell biology, Vol. 155, Issue 3, pp. 331-8, 2001

Dienz, Hehner, Droge, Schmitz: "Synergistic activation of NF-kappa B by functional cooperation between vav and PKCtheta in T lymphocytes." in: The Journal of biological chemistry, Vol. 275, Issue 32, pp. 24547-51, 2000

Villalba, Coudronniere, Deckert, Teixeiro, Mas, Altman: "A novel functional interaction between Vav and PKCtheta is required for TCR-induced T cell activation." in: Immunity, Vol. 12, Issue 2, pp. 151-60, 2000

Soderling: "Protein kinases. Regulation by autoinhibitory domains." in: The Journal of biological chemistry, Vol. 265, Issue 4, pp. 1823-6, 1990

Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, 1988

Images

Product Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling References for anti-PKC theta Antibody (ABIN967738) back to top
Supplier Images
Western Blotting (WB) image for anti-PKC theta antibody (Protein Kinase C, theta) (AA 21-217) (ABIN967738) Western blot analysis of PKCtheta on a Jurkat lysate. Lane 1: 1:250, lane 2: 1:500, l...
Immunofluorescence (IF) image for anti-PKC theta antibody (Protein Kinase C, theta) (AA 21-217) (ABIN967738) Immunofluorescence staining of A431 cells.