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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||27 Antibodies||27 Antibodies|
|Immunochromatography (IC)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||3 Antibodies|
|Immunofluorescence (IF)||23 Antibodies||23 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||40 Antibodies||40 Antibodies|
|Immunohistochemistry (IHC)||54 Antibodies||59 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||30 Antibodies||32 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||1 Antibodies|
|Western Blotting (WB)||85 Antibodies||90 Antibodies|
|Antigen||Protein Kinase C, theta (PRKCQ) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PKC theta antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-PKC theta AntibodyTarget Details PKC theta Application Details Handling Images
|Specificity||This antibody detects endogenous levels of PKC theta protein only when phosphorylated at Ser676.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details PKC thetaProduct Details anti-PKC theta Antibody Application Details Handling Images back to top
|Alternative Name||PRKCQ / PRKCT (PRKCQ Antibody Abstract)|
|Background||Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (alpha, betaI, betaII, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, my and ny). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies.Synonyms: PKC theta, PKC-theta, Protein kinase C theta type, Protein kinase C theta type, nPKC-theta|
|Molecular Weight||approx. 80 kDa|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling|
Application DetailsProduct Details anti-PKC theta Antibody Target Details PKC theta Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC theta Antibody Target Details PKC theta Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-PKC theta Antibody Target Details PKC theta Application Details Handling back to top