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anti-Human ZAP70 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • 70kDa
  • AI327364
  • MGC80670
  • MGC88962
  • mrtle
  • mur
  • SRK
  • Srk
  • STCD
  • STD
  • TZK
  • ZAP-70
  • zap70
  • ZAP70
Epitope
pTyr493
64
50
47
46
25
22
21
21
20
15
15
15
14
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
530
185
121
4
4
3
2
2
2
Host
Mouse
298
212
15
8
3
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is un-conjugated
31
24
18
16
10
8
8
7
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
296
163
155
148
121
68
54
44
24
7
5
5
4
4
2
2
Supplier
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Catalog No. ABIN967585
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN1027649 IHC (f) IHC (p) Mouse IgG2a Log in to see 2F3-2
0.0008709465 ABIN1027650 IHC (f) IHC (p) Mouse IgG2a Log in to see 2F3-2

Top referenced anti-ZAP70 antibody for Immunohistochemistry (Formalin-fixed Sections)

Similar anti-ZAP70 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 40 Antibodies 160 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 21 Antibodies 150 Antibodies
Immunochromatography (IC) 3 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 19 Antibodies 25 Antibodies
Immunofluorescence (IF) 33 Antibodies 69 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies
Immunohistochemistry (IHC) 67 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Western Blotting (WB) 109 Antibodies
FLISA 2 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope pTyr493
(64), (50), (47), (46), (25), (22), (21), (21), (20), (15), (15), (15), (14), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(530), (185), (121), (4), (4), (3), (2), (2), (2)
Host Mouse
(298), (212), (15), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is un-conjugated
(31), (24), (18), (16), (10), (8), (8), (7), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
(296), (163), (155), (148), (121), (68), (54), (44), (24), (7), (5), (5), (4), (4), (2), (2)
Pubmed 3 references available
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN967585) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Clone 1a-Zap70
Isotype IgG1, kappa

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 Antibody (ABIN967585) Images back to top
Antigen
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background The 70-kDa zeta chain-associated protein (ZAP70) is a protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both ZAP70 and Syk are recruited to the phosphorylated CD3 and zeta subunits after TCR stimulation. TCR-mediated Lck activity leads to phosphorylation of ZAP70 on Tyrosine 493 (Y493) in the regulatory loop of the kinase domain leading to upregulation of ZAP70 kinase activity. The significance of ZAP70 activation in mediating TCR signal transduction has been confirmed by showing that ZAP70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP70 kinase domain which affect the stability of the protein and TCR signaling. The 1a/Zap70 (pY493) antibody recognizes ZAP70 phosphorylated at Y493.
Molecular Weight 70 kDa
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 Antibody (ABIN967585) Images back to top
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 Antibody (ABIN967585) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-ZAP70 Antibody (ABIN967585)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Product cited in:

Mège, Di Bartolo, Germain, Tuosto, Michel, Acuto: "Mutation of tyrosines 492/493 in the kinase domain of ZAP-70 affects multiple T-cell receptor signaling pathways." in: The Journal of biological chemistry, Vol. 271, Issue 51, pp. 32644-52, 1997

Chan, Dalton, Johnson, Kong, Wang, Thoma, Kurosaki: "Activation of ZAP-70 kinase activity by phosphorylation of tyrosine 493 is required for lymphocyte antigen receptor function." in: The EMBO journal, Vol. 14, Issue 11, pp. 2499-508, 1995

Chan, Kadlecek, Elder, Filipovich, Kuo, Iwashima, Parslow, Weiss: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN967585) back to top
Supplier Images
Western Blotting (WB) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493) (ABIN967585) Western blot analysis of ZAP70 (pY493). Lysates from control (left panel) and hydroge...
 image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493) (ABIN967585) ZAP70 staining on tonsil. Fresh human tonsil was incubated in 5 mM Pervanadate soluti...
Western Blotting (WB) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493) (ABIN967585) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody (Image 3)