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anti-Mouse (Murine) ZAP70 antibody for Flow Cytometry

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta-chain (TCR) associated protein kinase 70kDa
  • tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta-chain (TCR) associated protein kinase 70
  • zap70
  • ZAP70
  • Smp_149470
  • Zap70
Reactivity
Human, Mouse (Murine)
526
213
139
12
10
10
9
7
5
4
4
3
1
Host
Mouse
297
207
15
8
3
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is conjugated to FITC
27
22
18
17
12
8
8
7
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
1
Application
Flow Cytometry (FACS)
295
161
143
140
128
64
52
46
20
7
5
5
4
4
4
3
3
2
2
Supplier
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Available images

Catalog No. ABIN2662039
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.775298 ABIN2663949 FACS IP IHC PE Mouse IgG1 kappa, IgG1 Log in to see 1E7-2 4
1 ABIN205364 FACS IP WB Mouse IgG1 AA 282-307 Log in to see 1E7-2
1 ABIN2665463 FACS Mouse IgG1 kappa, IgG1 Log in to see 1E7-2 5
1 ABIN2663950 FACS IP IHC PE Mouse IgG1 kappa, IgG1 Log in to see 1E7-2 4
1 ABIN218908 FACS IP ELISA WB Rabbit IgG pTyr493 Log in to see Polyclonal
1 ABIN221118 ICC FACS IF IP WB Rabbit IgG pTyr319 Log in to see Polyclonal
1 ABIN4366560 FACS ICC IF WB DyLight 680 Mouse IgG1 Log in to see 1E7-2
1 ABIN4366561 FACS ICC IF WB DyLight 488 Mouse IgG1 Log in to see 1E7-2
1 ABIN4366565 FACS PE Mouse IgG1 Log in to see 1E7-2
1 ABIN4366564 FACS ICC IF IP WB PerCP Mouse IgG1 Log in to see 1E7-2
1 ABIN4366567 FACS ICC IF WB Alexa Fluor 405 Mouse IgG1 Log in to see 1E7-2
1 ABIN4366562 FACS ICC IF WB DyLight 350 Mouse IgG1 Log in to see 1E7-2
1 ABIN4366570 FACS ICC IF WB Alexa Fluor 700 Mouse IgG1 Log in to see 1E7-2
1 ABIN4366568 FACS ICC IF WB Alexa Fluor 488 Mouse IgG1 Log in to see 1E7-2
1 ABIN4366569 FACS ICC IF WB Alexa Fluor 647 Mouse IgG1 Log in to see 1E7-2
1 ABIN4366555 CyTOF FACS ICC IF IP WB Mouse IgG1 Log in to see 1E7-2
1 ABIN4366558 FACS ICC IF WB DyLight 405 Mouse IgG1 Log in to see 1E7-2
1 ABIN4366559 FACS ICC IF WB DyLight 755 Mouse IgG1 Log in to see 1E7-2
1 ABIN4366563 FACS Allophycocyanin Mouse IgG1 Log in to see 1E7-2
1 ABIN1959471 FACS IP Rabbit IgG pTyr319 Log in to see Polyclonal

Top referenced anti-ZAP70 antibody for Flow Cytometry

Similar anti-ZAP70 Antibodies

Application / Reactivity Human Mouse (Murine)
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 2 Antibodies
Cytometry by Time of Flight (CyTOF) 5 Antibodies 1 Antibodies
Simple Western (SimWes) 5 Antibodies
Western Blotting (WB) 291 Antibodies 126 Antibodies
Intracellular Staining (ICS) 4 Antibodies
Immunoprecipitation (IP) 46 Antibodies 17 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 128 Antibodies 29 Antibodies
Immunohistochemistry (IHC) 143 Antibodies 64 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 52 Antibodies 52 Antibodies
Immunofluorescence (IF) 63 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Reactivity Human, Mouse (Murine)
(526), (213), (139), (12), (10), (10), (9), (7), (5), (4), (4), (3), (1)
Host Mouse
(297), (207), (15), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is conjugated to FITC
(27), (22), (18), (17), (12), (8), (8), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1)
Application Flow Cytometry (FACS)
(295), (161), (143), (140), (128), (64), (52), (46), (20), (7), (5), (5), (4), (4), (4), (3), (3), (2), (2)
Pubmed 4 references available
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN2662039) Images
Purification The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Clone 1E7-2
Isotype IgG1 kappa, IgG1

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 Antibody (ABIN2662039) Images back to top
Antigen
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background ZAP-70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP-70 have been linked to selective T cell defects. The ZAP-70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP-70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL). The 1E7.2 monoclonal antibody recognizes human and mouse ZAP-70 and has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 Antibody (ABIN2662039) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 Antibody (ABIN2662039) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-ZAP70 Antibody (ABIN2662039)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Background publications

Crespo, Bosch, Villamor, Bellosillo, Colomer, Rozman, Marcé, López-Guillermo, Campo, Montserrat: "ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia." in: The New England journal of medicine, Vol. 348, Issue 18, pp. 1764-75, 2003

Qian, Lev, van Oers, Dikic, Schlessinger, Weiss: "Tyrosine phosphorylation of Pyk2 is selectively regulated by Fyn during TCR signaling." in: The Journal of experimental medicine, Vol. 185, Issue 7, pp. 1253-9, 1997

Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, 1994

Chan, Iwashima, Turck, Weiss: "ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain." in: Cell, Vol. 71, Issue 4, pp. 649-62, 1992

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN2662039) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa)  (FITC) (ABIN2662039) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody (FITC)