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|Application / Reactivity||Human||Mouse (Murine)|
|Western Blotting (WB)||278 Antibodies||94 Antibodies|
|Intracellular Staining (ICS)||5 Antibodies|
|Immunoprecipitation (IP)||42 Antibodies||8 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||64 Antibodies||21 Antibodies|
|Immunohistochemistry (IHC)||153 Antibodies||66 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||5 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||3 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||44 Antibodies||44 Antibodies|
|Immunofluorescence (IF)||39 Antibodies||19 Antibodies|
|Immunocytochemistry (ICC)||11 Antibodies||5 Antibodies|
|Immunochromatography (IC)||7 Antibodies||3 Antibodies|
|Antigen||zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This ZAP70 antibody is conjugated to FITC Alternatives|
Flow Cytometry (FACS)
|4 references available|
|Supplier||Log in to see|
Product Details anti-ZAP70 AntibodyTarget Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN2662039) Images
|Purification||The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.|
|Isotype||IgG1 kappa, IgG1|
Target Details ZAP70Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 Antibody (ABIN2662039) Images back to top
|Alternative Name||ZAP-70 (ZAP70 Antibody Abstract)|
|Background||ZAP-70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP-70 have been linked to selective T cell defects. The ZAP-70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP-70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL). The 1E7.2 monoclonal antibody recognizes human and mouse ZAP-70 and has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.|
|Research Area||Signaling, Tags/Labels, Immunology, Cancer|
Application DetailsProduct Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 Antibody (ABIN2662039) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 Antibody (ABIN2662039) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Protect from prolonged exposure to light. Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-ZAP70 Antibody (ABIN2662039)Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Crespo, Bosch, Villamor et al.: "ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia." in: The New England journal of medicine, Vol. 348, Issue 18, pp. 1764-75, 2003 (PubMed).
Qian, Lev, van Oers et al.: "Tyrosine phosphorylation of Pyk2 is selectively regulated by Fyn during TCR signaling." in: The Journal of experimental medicine, Vol. 185, Issue 7, pp. 1253-9, 1997 (PubMed).
Arpaia, Shahar, Dadi et al.: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, 1994 (PubMed).
Chan, Iwashima, Turck et al.: "ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain." in: Cell, Vol. 71, Issue 4, pp. 649-62, 1992 (PubMed).
ImagesProduct Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN2662039) back to top