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anti-Rat (Rattus) ZAP70 antibody for Immunohistochemistry

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta-chain (TCR) associated protein kinase 70kDa
  • tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta-chain (TCR) associated protein kinase 70
  • zap70
  • ZAP70
  • Smp_149470
  • Zap70
Epitope
pTyr493
62
51
50
39
31
23
23
14
14
13
9
9
9
8
8
8
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
548
226
148
12
10
10
9
7
6
4
4
3
1
Host
Rabbit
309
216
16
8
3
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
30
23
18
17
12
8
8
7
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
308
168
152
147
128
64
52
45
20
7
5
5
4
4
4
3
3
2
2
Options
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Catalog No. ABIN1532002
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.72946 ABIN1533039 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
8.72946 ABIN1533040 IHC ELISA Rabbit IgG Log in to see Polyclonal
8.72946 ABIN1870702 IHC WB Rabbit IgG pTyr493 Log in to see Polyclonal
8.72946 ABIN2501280 IHC Rabbit IgG Tyr493 Log in to see Polyclonal
8.72946 ABIN2501290 IHC ELISA WB Rabbit IgG Tyr319 Log in to see Polyclonal
8.72946 ABIN2501281 IHC Rabbit IgG pTyr493 Log in to see Polyclonal
8.72946 ABIN2501284 IHC WB Rabbit IgG Tyr319 Log in to see Polyclonal
8.72946 ABIN1875382 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
8.72946 ABIN2501291 IHC ELISA WB Rabbit IgG pTyr493 Log in to see Polyclonal
8.72946 ABIN2501285 IHC ELISA Rabbit IgG Tyr493 Log in to see Polyclonal
8.72946 ABIN2469923 IHC WB Mouse full length Log in to see 11H40
1 ABIN967848 IF IP IHC WB Mouse IgG2a AA 468-619 Log in to see 29-ZAP70 Kinase 5
1 ABIN967849 IF IP IHC WB Mouse IgG2a AA 468-619 Log in to see 29-ZAP70 Kinase 5
1 ABIN1870700 IF IHC WB Rabbit IgG pTyr319 Log in to see Polyclonal
1 ABIN362166 IHC Rabbit IgG pTyr493 Log in to see Polyclonal 3
1 ABIN362798 IHC Rabbit IgG AA 491-495 Log in to see Polyclonal 3
1 ABIN362796 IHC WB Rabbit IgG AA 317-321 Log in to see Polyclonal 6
1 ABIN2889082 ELISA IHC WB Rabbit IgG AA 286-335 Log in to see Polyclonal
1 ABIN3023184 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN3182176 ELISA IHC WB Rabbit IgG pTyr493 Log in to see Polyclonal

Top referenced anti-ZAP70 antibody for Immunohistochemistry

Similar anti-ZAP70 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 25 Antibodies 53 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 22 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 16 Antibodies
Immunofluorescence (IF) 16 Antibodies 32 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies 52 Antibodies
Immunohistochemistry (IHC) 61 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 85 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope pTyr493
(62), (51), (50), (39), (31), (23), (23), (14), (14), (13), (9), (9), (9), (8), (8), (8), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(548), (226), (148), (12), (10), (10), (9), (7), (6), (4), (4), (3), (1)
Host Rabbit
(309), (216), (16), (8), (3)
Clonality
Polyclonal
Conjugate This ZAP70 antibody is un-conjugated
(30), (23), (18), (17), (12), (8), (8), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(308), (168), (152), (147), (128), (64), (52), (45), (20), (7), (5), (5), (4), (4), (4), (3), (3), (2), (2)
Pubmed 2 references available
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN1532002) Images
Specificity ZAP-70 (Phospho-Tyr493) Antibody detects endogenous levels of ZAP- 70 only when phosphorylated at Tyr493.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyr493.
Isotype IgG

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 Antibody (ABIN1532002) Images back to top
Antigen
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background Molecular weight of target protein: 69 kDa
Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70, ZAP70, kinase ZAP70
Molecular Weight 69 kDa
Gene ID 7535
OMIM 176947
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 Antibody (ABIN1532002) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:4000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.234569 (NCBI Gene Symbol: ZAP70)

Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 Antibody (ABIN1532002) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-ZAP70 Antibody (ABIN1532002)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Background publications

Hillier, Graves, Fulton, Fulton, Pepin, Minx, Wagner-McPherson, Layman, Wylie, Sekhon, Becker, Fewell, Delehaunty, Miner, Nash, Kremitzki, Oddy, Du, Sun, Bradshaw-Cordum, Ali, Carter, Cordes, Harris et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005

Chan, Iwashima, Turck, Weiss: "ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain." in: Cell, Vol. 71, Issue 4, pp. 649-62, 1992

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling References for anti-ZAP70 Antibody (ABIN1532002) back to top
Supplier Images
Western Blotting (WB) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493) (ABIN1532002) Western blot analysis of extracts from Jurkat cells, using ZAP-70 (Phospho-Tyr493) An...
Immunohistochemistry (IHC) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493) (ABIN1532002) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ZAP-...