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anti-Dog (Canine) AES antibody for Western Blotting

Recommended AES Antibody (supplied by: Log in to see )

Antigen
Amino-terminal Enhancer of Split (AES) Antibodies
  • AES
  • AES-1
  • aes-1
  • AES-2
  • aes-2
  • AL024115
  • esp1
  • Esp1
  • ESP1
  • Grg
  • GRG
  • grg
  • Grg-5
  • Grg5
  • GRG5
  • grg5
  • tle5
  • Tle5
  • TLE5
  • xgrg-5
  • zgc:64216
Epitope
C-Term
43
24
15
4
3
3
2
2
2
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
91
34
22
2
2
2
1
1
Host
Rabbit
75
12
7
Clonality
Polyclonal
Conjugate
This AES antibody is un-conjugated
4
4
4
4
4
4
1
Application
Western Blotting (WB)
81
42
13
9
6
5
3
3
2
1
1
1
Supplier
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Catalog No. ABIN2774824
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398065 ABIN2461366 ELISA WB Rabbit Log in to see Polyclonal
0.13398065 ABIN629632 WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-AES antibody for Western Blotting

Similar anti-AES Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine)
Western Blotting (WB) 81 Antibodies 31 Antibodies 20 Antibodies 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies 2 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 7 Antibodies 4 Antibodies 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies

General

Antigen Amino-terminal Enhancer of Split (AES) Antibodies
Epitope C-Term
(43), (24), (15), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(91), (34), (22), (2), (2), (2), (1), (1)
Host Rabbit
(75), (12), (7)
Clonality
Polyclonal
Conjugate This AES antibody is un-conjugated
(4), (4), (4), (4), (4), (4), (1)
Application Western Blotting (WB)
(81), (42), (13), (9), (6), (5), (3), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-AES Antibody

Target Details AES Application Details Handling References for anti-AES Antibody (ABIN2774824) Images
Sequence GLQPPSLPAV SAGTGLLSLS ALGSQAHLSK EDKNGHDGDT HQEDDGEKSD
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Human : 100 %, Mouse : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against AES. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human AES

Target Details AES

Product Details anti-AES Antibody Application Details Handling References for anti-AES Antibody (ABIN2774824) Images back to top
Antigen
Alternative Name AES (AES Antibody Abstract)
Background AES is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genesThe protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 22 kDa
Gene ID 166
NCBI Accession NP_001121, NM_001130
UniProt Q08117
Pathways WNT Signaling

Application Details

Product Details anti-AES Antibody Target Details AES Handling References for anti-AES Antibody (ABIN2774824) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 197 AA

Restrictions For Research Use only

Handling

Product Details anti-AES Antibody Target Details AES Application Details References for anti-AES Antibody (ABIN2774824) Images back to top
Format Liquid
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-AES Antibody (ABIN2774824)

Product Details anti-AES Antibody Target Details AES Application Details Handling Images back to top
Background publications

Jan, Matter, Pai, Chen, Pilch, Komatsu, Ong, Fukuda, Ruoslahti: "A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors." in: Cell, Vol. 116, Issue 5, pp. 751-62, 2004

Images

Product Details anti-AES Antibody Target Details AES Application Details Handling References for anti-AES Antibody (ABIN2774824) back to top
Supplier Images
Western Blotting (WB) image for anti-AES antibody (Amino-terminal Enhancer of Split) (C-Term) (ABIN2774824) WB Suggested Anti-AES Antibody Titration: 2.5ug/ml
Positive Control: